Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACOB85_RS10470 Genome accession   NZ_CP184834
Coordinates   565684..567369 (-) Length   561 a.a.
NCBI ID   WP_420788407.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain HoVa25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 560684..572369
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOB85_RS10440 (ACOB85_10440) rplS 560734..561087 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  ACOB85_RS10445 (ACOB85_10445) yacG 561767..561961 (-) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  ACOB85_RS10450 (ACOB85_10450) zapD 562120..562860 (-) 741 WP_005373086.1 cell division protein ZapD -
  ACOB85_RS10455 (ACOB85_10455) coaE 562888..563502 (-) 615 WP_420788405.1 dephospho-CoA kinase -
  ACOB85_RS10460 (ACOB85_10460) pilD 563503..564372 (-) 870 WP_420788406.1 prepilin peptidase Machinery gene
  ACOB85_RS10465 (ACOB85_10465) pilC 564437..565660 (-) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  ACOB85_RS10470 (ACOB85_10470) pilB 565684..567369 (-) 1686 WP_420788407.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACOB85_RS10475 (ACOB85_10475) pilA 567382..567813 (-) 432 WP_420788408.1 pilin Machinery gene
  ACOB85_RS10480 (ACOB85_10480) nadC 568069..568956 (-) 888 WP_005379983.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACOB85_RS10485 (ACOB85_10485) ampD 569049..569600 (+) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACOB85_RS10490 (ACOB85_10490) pdhR 570006..570773 (+) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62148.96 Da        Isoelectric Point: 5.5338

>NTDB_id=1111381 ACOB85_RS10470 WP_420788407.1 565684..567369(-) (pilB) [Vibrio alginolyticus strain HoVa25]
MSRNLSTVLRQAGILTFSQEESLLEQVKASGISMPEALLSSELLTSSELADHLSSLFSLNQPELSQYDYASLCQQLGLRE
LITRHNALPLHRTPSTLLLAVADPTNQQAEDDFRFATGLQVELVLANFRQLSAAIRRLYGRALGQEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1111381 ACOB85_RS10470 WP_420788407.1 565684..567369(-) (pilB) [Vibrio alginolyticus strain HoVa25]
ATGTCTAGAAACCTCTCAACCGTTCTTCGCCAAGCAGGAATACTGACCTTTTCCCAAGAAGAATCGTTGCTTGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCGGAAGCTTTACTCAGTTCTGAATTATTGACATCAAGCGAACTCGCTGATCACTTAA
GTTCTCTCTTTAGTTTAAATCAACCCGAGTTATCTCAGTACGATTACGCTTCGCTTTGTCAGCAACTTGGTCTTAGGGAA
TTAATCACACGGCATAACGCACTCCCACTCCACCGTACGCCTTCAACTTTATTATTAGCGGTTGCCGACCCCACGAATCA
ACAAGCTGAAGATGATTTCCGCTTTGCCACTGGGTTACAGGTTGAACTGGTCTTGGCGAATTTTCGTCAACTCAGCGCTG
CGATTCGACGCTTGTATGGCCGCGCACTTGGGCAGGAAAAGTCTGGGCTAAAAGAGATCAACCAAGAAGAGCTAGCCAAC
CTAGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATCAA
CCAAATTCTATTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGCGTGCGCC
TGCGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTGAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTACCTACTCTATTCGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCGTCAC
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACAGGGAGCGGTAAAACCGTTTCTCTATACACTGGGCTAAGTATCCTCAATAAGCCAGAAATCAA
CATTTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTCTTACGTCAAGACCCGGATGTGGTAATGGTCGGTGAGATTCGTGACTTAGACACC
GCCGAAATCGCTATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTGCATACCAATTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAGTTTGATTATTGCCCAGCGCTTGGCCC
GTAAACTGTGCTCGCACTGTAAACAATCACAAGAGCTTACCGTTCAGCTACAACACCTTGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGTAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGCGCCTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

94.118

100

0.941
  pilB Vibrio campbellii strain DS40M4

89.661

100

0.897
  pilB Vibrio cholerae strain A1552

73.488

100

0.736
  pilB Legionella pneumophila strain ERS1305867

48.746

99.465

0.485
  pilB Acinetobacter baumannii D1279779

51.81

93.583

0.485
  pilB Acinetobacter baylyi ADP1

50.895

89.661

0.456
  pilF Neisseria gonorrhoeae MS11

44.681

100

0.449
  pilB/pilB1 Synechocystis sp. PCC 6803

37.688

100

0.401
  pilF Thermus thermophilus HB27

36.155

100

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.364

100

0.364

Multiple sequence alignment