Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACOC81_RS04910 Genome accession   NZ_CP184649
Coordinates   1027989..1029206 (-) Length   405 a.a.
NCBI ID   WP_004887449.1    Uniprot ID   A0A0P9ZIN2
Organism   Pseudomonas viridiflava strain p21.B10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1022989..1034206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOC81_RS04875 (ACOC81_04875) - 1023944..1024117 (-) 174 WP_069145033.1 DUF3094 family protein -
  ACOC81_RS04880 (ACOC81_04880) - 1024131..1024676 (-) 546 WP_122439232.1 MOSC domain-containing protein -
  ACOC81_RS04885 (ACOC81_04885) - 1024673..1025302 (-) 630 WP_004884092.1 DUF1780 domain-containing protein -
  ACOC81_RS04890 (ACOC81_04890) - 1025427..1025888 (+) 462 WP_069145034.1 hypothetical protein -
  ACOC81_RS04895 (ACOC81_04895) yacG 1026289..1026498 (-) 210 WP_122439230.1 DNA gyrase inhibitor YacG -
  ACOC81_RS04900 (ACOC81_04900) coaE 1026495..1027118 (-) 624 WP_025993968.1 dephospho-CoA kinase -
  ACOC81_RS04905 (ACOC81_04905) pilD 1027115..1027987 (-) 873 WP_122439229.1 prepilin peptidase Machinery gene
  ACOC81_RS04910 (ACOC81_04910) pilC 1027989..1029206 (-) 1218 WP_004887449.1 type II secretion system F family protein Machinery gene
  ACOC81_RS04915 (ACOC81_04915) pilB 1029209..1030909 (-) 1701 WP_004887450.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACOC81_RS04920 (ACOC81_04920) - 1031136..1031558 (+) 423 WP_004887452.1 pilin -
  ACOC81_RS04930 (ACOC81_04930) - 1031753..1032733 (-) 981 WP_122447119.1 YiiG family protein -
  ACOC81_RS04935 (ACOC81_04935) - 1032789..1033220 (-) 432 WP_122447118.1 hypothetical protein -
  ACOC81_RS04940 (ACOC81_04940) - 1033455..1034006 (-) 552 WP_122447117.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44034.94 Da        Isoelectric Point: 10.1642

>NTDB_id=1110561 ACOC81_RS04910 WP_004887449.1 1027989..1029206(-) (pilC) [Pseudomonas viridiflava strain p21.B10]
MASKAVKVSVFTWEGVDKKGGKISGELSGHNPALIKAQLRKQGINPTKVRKKTVSIFGRGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGSENPNMRSLVDSLKQEVSAGNSFATALRQKPEHFDDLFCNLVDAGEQAGALESLLDRVATYKEKT
EKLKAKIKKAMTYPAAVLVVAIIVSGILLIKVVPQFQSVFASFGAELPAFTLMVIGLSEIVQTWWLAILGLFIAGIFLFK
RSYKQSQKFRDSIDRFLLKIPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVMKIKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDSMLDKVATYYEDEVDNMVDSLTSLMEPMIMAVLGVIVGGLVIAMYLPIFK
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1110561 ACOC81_RS04910 WP_004887449.1 1027989..1029206(-) (pilC) [Pseudomonas viridiflava strain p21.B10]
ATGGCCAGTAAAGCAGTCAAAGTCAGTGTTTTCACCTGGGAAGGCGTAGACAAGAAAGGCGGCAAGATCAGCGGTGAATT
GAGTGGCCACAATCCGGCGCTGATCAAGGCTCAACTTCGTAAGCAGGGCATCAACCCGACTAAAGTGAGGAAGAAAACGG
TCTCGATCTTCGGCAGGGGTAAAAAGATCAAACCGCTGGACATCGCTTTTTTCTCTCGGCAAATGGCGACGATGATGAAG
GCTGGCGTTCCACTGCTGCAGTCGTTCGACATCATCAGCGAAGGTTCGGAGAATCCGAACATGCGCTCTCTTGTCGACTC
CCTGAAACAAGAGGTATCGGCCGGTAACAGTTTTGCGACCGCCCTCAGACAAAAGCCAGAACACTTCGATGATCTTTTCT
GCAACTTGGTGGACGCTGGCGAGCAGGCGGGAGCTCTGGAAAGCCTGCTGGACCGTGTTGCGACCTACAAGGAAAAAACC
GAAAAGCTGAAAGCCAAGATCAAGAAAGCCATGACTTATCCAGCTGCGGTTTTGGTGGTGGCTATCATCGTTTCCGGTAT
TTTGCTCATCAAAGTGGTTCCCCAGTTCCAATCAGTCTTTGCCAGTTTCGGAGCGGAACTGCCTGCTTTCACGCTCATGG
TGATAGGGCTTTCGGAGATAGTACAAACTTGGTGGCTTGCAATTCTTGGCCTTTTCATCGCGGGCATTTTTCTGTTCAAA
CGGTCCTATAAACAGTCACAGAAGTTTCGCGACAGTATTGACAGATTTTTGTTGAAAATCCCTATTATTGGGCCGTTGAT
ATTTAAGTCGTCGGTGGCGCGGTACGCTCGGACACTCGCAACCACTTTTGCTGCAGGCGTCCCGCTAGTCGAGGCCCTAG
ACTCGGTTGCGGGCGCGACCGGTAATGTCGTGTTCAGAAATGCGGTCATGAAGATCAAGCAAGATGTCTCAACGGGCATG
CAACTAAACTTTTCGATGCGCTCCACTGGCGTTTTCCCGAGCCTTGCCATCCAGATGACCGCCATCGGGGAAGAGTCCGG
AGCACTGGACAGTATGCTCGACAAGGTCGCCACCTATTACGAAGATGAAGTCGATAACATGGTCGACAGCCTGACCAGCC
TCATGGAGCCAATGATCATGGCGGTACTTGGGGTCATCGTCGGCGGTCTGGTGATCGCCATGTATCTGCCGATATTCAAA
CTTGGAAACGTTGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0P9ZIN2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.309

100

0.753
  pilC Acinetobacter baumannii D1279779

61.634

99.753

0.615
  pilC Acinetobacter baylyi ADP1

60.539

100

0.61
  pilC Legionella pneumophila strain ERS1305867

52.778

97.778

0.516
  pilG Neisseria gonorrhoeae MS11

43.812

99.753

0.437
  pilG Neisseria meningitidis 44/76-A

43.812

99.753

0.437
  pilC Vibrio cholerae strain A1552

41.728

100

0.417
  pilC Vibrio campbellii strain DS40M4

40.976

100

0.415
  pilC Thermus thermophilus HB27

39.401

99.012

0.39

Multiple sequence alignment