Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACOC81_RS04915 Genome accession   NZ_CP184649
Coordinates   1029209..1030909 (-) Length   566 a.a.
NCBI ID   WP_004887450.1    Uniprot ID   -
Organism   Pseudomonas viridiflava strain p21.B10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1024209..1035909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOC81_RS04885 (ACOC81_04885) - 1024673..1025302 (-) 630 WP_004884092.1 DUF1780 domain-containing protein -
  ACOC81_RS04890 (ACOC81_04890) - 1025427..1025888 (+) 462 WP_069145034.1 hypothetical protein -
  ACOC81_RS04895 (ACOC81_04895) yacG 1026289..1026498 (-) 210 WP_122439230.1 DNA gyrase inhibitor YacG -
  ACOC81_RS04900 (ACOC81_04900) coaE 1026495..1027118 (-) 624 WP_025993968.1 dephospho-CoA kinase -
  ACOC81_RS04905 (ACOC81_04905) pilD 1027115..1027987 (-) 873 WP_122439229.1 prepilin peptidase Machinery gene
  ACOC81_RS04910 (ACOC81_04910) pilC 1027989..1029206 (-) 1218 WP_004887449.1 type II secretion system F family protein Machinery gene
  ACOC81_RS04915 (ACOC81_04915) pilB 1029209..1030909 (-) 1701 WP_004887450.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACOC81_RS04920 (ACOC81_04920) - 1031136..1031558 (+) 423 WP_004887452.1 pilin -
  ACOC81_RS04930 (ACOC81_04930) - 1031753..1032733 (-) 981 WP_122447119.1 YiiG family protein -
  ACOC81_RS04935 (ACOC81_04935) - 1032789..1033220 (-) 432 WP_122447118.1 hypothetical protein -
  ACOC81_RS04940 (ACOC81_04940) - 1033455..1034006 (-) 552 WP_122447117.1 DUF1523 family protein -
  ACOC81_RS04945 (ACOC81_04945) - 1034126..1035124 (+) 999 WP_122447116.1 ATP-grasp domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62664.77 Da        Isoelectric Point: 6.3861

>NTDB_id=1110562 ACOC81_RS04915 WP_004887450.1 1029209..1030909(-) (pilB) [Pseudomonas viridiflava strain p21.B10]
MSDVVVLTGLAKQLVAAELLSEKAAQQAFQQARRDKISLVSYLVQNKLVKSLTLAEMASEQFGVPFMDLSSLDKENQPKG
LVSEKLARQHHALPLWRRGNRLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLSVAIDRFFDSDSGLGSLADIDLDL
DIEPGESAKETSITSRDDADDAPVVRFVNKMLLDAIRLGSSDLHFEPYEKVFRVRFRTDGILHEAARPPINLANRIAARL
KVMASLDISERRKPQDGRVKLRVSKTKSIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPDQKELYLEALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVNINISTAEDPVEINLEGVNQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKEVDVPHETLIKEGFPE
SKVGTFKLYGPVGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1110562 ACOC81_RS04915 WP_004887450.1 1029209..1030909(-) (pilB) [Pseudomonas viridiflava strain p21.B10]
ATGAGTGATGTCGTTGTCCTAACAGGTCTGGCCAAGCAATTGGTCGCCGCCGAGCTCCTTTCCGAAAAAGCTGCCCAGCA
GGCATTCCAGCAGGCGCGACGCGACAAGATTTCGCTGGTCAGCTATCTGGTCCAGAACAAACTGGTCAAGAGTCTGACCC
TCGCAGAAATGGCCTCGGAGCAGTTCGGCGTGCCGTTCATGGACCTCTCCAGCCTGGATAAAGAAAACCAGCCCAAAGGC
CTGGTAAGCGAAAAGCTCGCTCGTCAGCATCACGCGCTGCCGCTATGGCGTCGCGGCAACCGACTCTTTATAGGCATATC
GGACCCGACCAATCATCAGGCTGTCACCGATATTCAGTTCAGCACTGGCCTGAACACTGAAGCGATTCTGGTCGAAGATG
ACAAACTCAGTGTTGCCATCGACAGGTTCTTTGACAGCGACAGCGGCTTGGGGTCATTGGCCGACATCGACTTGGATCTG
GATATAGAACCGGGCGAAAGCGCCAAAGAAACATCAATCACCAGCCGCGATGATGCCGATGACGCTCCCGTCGTCCGTTT
CGTCAATAAAATGCTGCTGGATGCCATTCGGCTGGGCTCGTCCGATTTGCACTTCGAACCTTATGAAAAGGTTTTCCGCG
TACGTTTTCGTACCGACGGGATTCTGCACGAAGCTGCCCGACCGCCGATCAATCTGGCCAACCGAATTGCTGCACGTCTA
AAGGTGATGGCCAGTCTGGACATTTCGGAACGGCGCAAGCCTCAGGACGGCAGGGTCAAGCTACGCGTGTCCAAAACCAA
ATCCATTGATTTCCGGATGAACACGCTGCCGACGCTGTGGGGTGAGAAGATCGTGATGCGGATCCTCGACCCGACCAGTG
CACAGATGGGCATCGATGCGCTGGGTTATGAACCGGATCAGAAAGAGCTGTATCTGGAAGCCCTTAAACAACCACAGGGC
ATGATTCTGGTGACCGGCCCGACCGGTTCGGGCAAAACCGTTTCGCTTTATACGGGCCTTAATATTCTTAACACTGTGAA
TATCAATATTTCAACCGCAGAAGACCCGGTGGAGATCAACCTTGAAGGCGTTAATCAGGTCAACGTCAACCCGCGTCAAG
GCATGGACTTCTCCCAGGCGTTGCGCGCGTTTCTGCGTCAGGACCCGGATGTGATCATGGTCGGTGAGATTCGTGACCTG
GAAACCGCCGAGATTGCGATCAAGGCCTCACAGACCGGCCACATGGTGTTGTCGACGCTGCACACCAACAGCGCTGCAGA
AACCCTGACACGCCTGCATCACATGGGCGTTGCGGCGTTCAACATCGCCACCGCTATCAACCTCATCATCGCCCAACGGC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGAAGGAAGTCGACGTACCCCATGAGACGCTGATCAAGGAAGGCTTCCCGGAG
TCGAAAGTCGGCACCTTCAAACTGTACGGCCCGGTCGGGTGCGAACACTGCAACGGCGGCTATCGAGGCCGAGTCGGTAT
TTACGAAGTCGTGAAAAAAACGCCGGAACTGGAGCGCATCATCATGGAAGAAGGCAACTCGCTGGAGATTTCCAGGCAGA
TGCGCAAGGATGGTTTCAATGACCTGCGCACATCAGGGTTGTCGAAAGCTATGCAGGGCATCACCAGCCTGGAAGAAGTC
AATCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.549

99.47

0.572
  pilB Acinetobacter baylyi ADP1

56.762

99.293

0.564
  pilB Legionella pneumophila strain ERS1305867

54.561

100

0.549
  pilB Vibrio cholerae strain A1552

50.265

99.823

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

49.818

97.173

0.484
  pilB Vibrio campbellii strain DS40M4

48.313

99.47

0.481
  pilF Neisseria gonorrhoeae MS11

47.78

99.47

0.475
  pilF Thermus thermophilus HB27

38.779

98.41

0.382

Multiple sequence alignment