Detailed information NTG1108420

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACN079_RS20835
• Genome accession: NZ_CP184478
• Coordinates: 4595611..4596729 (+)
• Length: 372 a.a.
• NCBI ID: WP_420231649.1
• Uniprot ID: -
• Organism: Pseudomonas sp. ABY48
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4590611..4601729

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACN079_RS20820 (ACN079_20820)	-	4591563..4592477 (+)	915	WP_420231648.1	sugar kinase	-
ACN079_RS20825 (ACN079_20825)	-	4592617..4592892 (-)	276	WP_003182808.1	peptidylprolyl isomerase	-
ACN079_RS20830 (ACN079_20830)	-	4592941..4595478 (-)	2538	WP_420235437.1	PAS domain-containing protein	-
ACN079_RS20835 (ACN079_20835)	pilU	4595611..4596729 (+)	1119	WP_420231649.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACN079_RS20840 (ACN079_20840)	-	4596818..4598773 (-)	1956	WP_420231650.1	acetoacetate--CoA ligase	-
ACN079_RS20845 (ACN079_20845)	-	4599002..4599778 (-)	777	WP_420231651.1	3-hydroxybutyrate dehydrogenase	-
ACN079_RS20850 (ACN079_20850)	-	4599786..4601177 (-)	1392	WP_420231652.1	GntP family permease	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41406.36 Da
• Isoelectric Point: 6.5106

>NTDB_id=1108420 ACN079_RS20835 WP_420231649.1 4595611..4596729(+) (pilU) [Pseudomonas sp. ABY48]
MEIDPLLRILASQNGSDLYMSTGAPPCARFEGVIKPLGNEAFKVGDIARLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDLSMVIRNVKLDIPRFEDLKLPRVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRSQLLNDLGNNLKAFVSQRLVRTRTGQRRAAVEVMLGSPTVADLIRRNEFGELKGIMEKSEELGMQTFDHAL
FNLVVEGAIDEQEALKNADSANNLRLRLKLHSESGAAAPPADPAAGEWGLMD

Nucleotide

• Length: 1119 bp

>NTDB_id=1108420 ACN079_RS20835 WP_420231649.1 4595611..4596729(+) (pilU) [Pseudomonas sp. ABY48]
ATGGAAATTGATCCCTTGTTGCGAATCCTGGCGAGCCAGAATGGTTCCGACCTTTACATGTCCACGGGTGCGCCACCGTG
CGCGCGCTTCGAGGGAGTGATCAAGCCCTTGGGGAACGAGGCATTCAAGGTCGGCGACATTGCCCGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGAGTTGGAAATGAACCTGGCGATCTCCCTGGCCGGTGTCGGGCGG
TTCCGGGTCAATATCTTCAAGCAGCGCAATGACCTGTCCATGGTGATCCGCAACGTCAAGCTGGACATCCCGCGCTTCGA
AGACCTGAAACTGCCCCGGGTGCTGTTGGACACGATCATGCAGAAACAAGGGCTGATGCTGTTCGTCGGCGCGACAGGTT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATTACCGCAATCGCAACAGCAGCGGTCACATCATCACCATCGAAGAC
CCGGTGGAGTATATCCATCGGCACAAGAAATCCATCATCAACCAACGGGAAGTCGGTGTCGACACCCGCAGTTTTCATGC
GGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAGATCCGCGACCGGGAAACCATGGAGCATG
CCTTGGCGTTTGCCGACACCGGGCACTTGGTGATTTCCACGCTGCATGCCCATAACGCTAATCAGGCCCTGGACCGGGTG
ATCAATTTCTTCCCGGAAGAACGTCGGTCGCAGTTGCTCAATGACCTGGGCAACAACCTCAAGGCGTTCGTCTCCCAACG
CCTGGTGCGTACCCGCACCGGCCAGCGGCGGGCGGCGGTGGAGGTCATGCTGGGCTCGCCCACGGTGGCCGACCTGATCC
GGCGTAACGAGTTCGGCGAGCTCAAGGGCATCATGGAAAAGTCAGAGGAGTTGGGGATGCAAACCTTCGATCATGCGCTG
TTCAACCTGGTGGTTGAAGGCGCCATCGATGAGCAAGAAGCCCTGAAAAACGCTGACTCGGCGAACAACCTGCGGTTGCG
GTTGAAGCTGCATAGCGAGTCGGGCGCGGCGGCGCCACCGGCCGATCCGGCGGCGGGTGAGTGGGGGCTTATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.62	95.43	0.54
pilU	Acinetobacter baylyi ADP1	51.966	95.699	0.497
pilU	Vibrio cholerae strain A1552	52.571	94.086	0.495
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	37.731	100	0.384
pilT	Vibrio cholerae strain A1552	41.141	89.516	0.368
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	89.516	0.368
pilT	Pseudomonas stutzeri DSM 10701	39.535	92.473	0.366
pilT	Acinetobacter baumannii D1279779	40.12	89.785	0.36
pilT	Acinetobacter baumannii strain A118	40.12	89.785	0.36
pilT	Acinetobacter nosocomialis M2	40.12	89.785	0.36