Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLKM0_RS12250 Genome accession   NZ_CP183907
Coordinates   2542081..2543307 (+) Length   408 a.a.
NCBI ID   WP_016659656.1    Uniprot ID   V2UF65
Organism   Acinetobacter indicus strain M3_NDM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2537081..2548307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM0_RS12210 rimP 2537528..2538052 (-) 525 WP_005181784.1 ribosome maturation factor RimP -
  ACLKM0_RS12235 secG 2538921..2539250 (-) 330 WP_005181783.1 preprotein translocase subunit SecG -
  ACLKM0_RS12240 tpiA 2539264..2540055 (-) 792 WP_411689004.1 triose-phosphate isomerase -
  ACLKM0_RS12245 pilB 2540344..2542050 (+) 1707 WP_160243588.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLKM0_RS12250 pilC 2542081..2543307 (+) 1227 WP_016659656.1 type II secretion system F family protein Machinery gene
  ACLKM0_RS12255 pilD 2543307..2544167 (+) 861 WP_005181774.1 prepilin peptidase Machinery gene
  ACLKM0_RS12260 coaE 2544178..2544786 (+) 609 WP_005181773.1 dephospho-CoA kinase -
  ACLKM0_RS12265 - 2544783..2545703 (-) 921 WP_016659657.1 DMT family transporter -
  ACLKM0_RS12270 rlmB 2545721..2546473 (-) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACLKM0_RS12275 - 2546579..2546902 (-) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44895.36 Da        Isoelectric Point: 9.9079

>NTDB_id=1107137 ACLKM0_RS12250 WP_016659656.1 2542081..2543307(+) (pilC) [Acinetobacter indicus strain M3_NDM]
MAVKKAQMMPTFIYEGIDRKGAKLKGELPARNMALAKVTLRKQGITIKTIRQKKKNILEGLMKKKVSTLDITIFTRQLAT
MMKAGVPLVQSFEIVAEGLENPAMREVVLGIKGEVEGGNTFAGALRKYPQHFDNLFCSLVESGEQSGALETMLDRVAIYK
EKSELLKQKIKKAMKYPATVIVVAVIVTIILMVKVVPVFEELFTSFGADLPAFTKMVVNMSQWTQKYWFVLIIVVGFAIA
AFLEAKKRSKKFRDLLDKAALRAPIFGDLVYKAIIARYSRTLATTFAAGVPLIDALESTAGATNNVVYEEAVMKIREDVA
TGQQLQFAMRVTDKFPSMAVQMVAIGEESGALDAMLDKVATHYENEVDNAVDGLTSMMEPLIMAVLGILVGGLVVAMYLP
IFQMGSVV

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=1107137 ACLKM0_RS12250 WP_016659656.1 2542081..2543307(+) (pilC) [Acinetobacter indicus strain M3_NDM]
ATGGCTGTCAAAAAAGCACAGATGATGCCCACCTTTATTTATGAAGGGATTGACCGCAAAGGGGCAAAACTTAAAGGGGA
ACTGCCAGCACGTAATATGGCGCTGGCCAAGGTCACGTTGCGCAAGCAAGGGATCACCATCAAAACCATCCGCCAGAAGA
AGAAAAATATTCTTGAAGGCTTGATGAAGAAAAAAGTCTCGACCCTGGACATCACGATTTTTACCCGCCAGCTGGCGACC
ATGATGAAAGCCGGTGTACCGCTGGTGCAAAGCTTTGAAATTGTGGCTGAAGGTCTGGAAAACCCGGCCATGCGTGAAGT
GGTATTGGGGATTAAAGGCGAAGTCGAAGGGGGCAATACCTTTGCTGGTGCACTGCGTAAATATCCGCAACATTTCGATA
ATCTGTTTTGCTCGCTGGTTGAATCGGGTGAACAGTCGGGTGCTCTAGAAACTATGCTGGACCGGGTAGCGATCTATAAA
GAAAAGAGCGAATTACTCAAACAAAAAATCAAGAAAGCCATGAAATACCCGGCAACGGTGATTGTGGTGGCGGTGATTGT
CACCATTATCCTGATGGTCAAAGTGGTACCGGTGTTTGAAGAACTCTTCACCTCTTTTGGTGCCGACTTACCAGCCTTCA
CCAAAATGGTGGTGAATATGTCGCAGTGGACCCAGAAATACTGGTTTGTGCTGATTATTGTGGTCGGCTTTGCGATTGCA
GCTTTTCTGGAAGCCAAGAAACGCAGCAAGAAATTCCGTGACCTGCTGGACAAAGCCGCCCTGAGAGCACCAATTTTTGG
TGATCTGGTGTATAAGGCGATTATCGCGCGTTATAGTCGTACCCTGGCCACCACCTTTGCTGCCGGTGTTCCCTTAATTG
ATGCACTGGAATCCACTGCCGGCGCCACCAACAACGTGGTTTATGAAGAAGCAGTAATGAAAATCCGTGAAGATGTCGCC
ACCGGTCAGCAGTTACAGTTTGCCATGCGGGTCACGGATAAATTCCCTTCGATGGCCGTGCAGATGGTTGCGATTGGTGA
AGAATCCGGTGCGCTGGATGCGATGCTGGATAAAGTCGCTACTCACTACGAAAACGAGGTCGATAACGCCGTCGATGGCT
TAACCTCGATGATGGAACCACTGATTATGGCCGTTCTCGGGATTCTGGTCGGCGGTCTGGTGGTCGCGATGTATCTCCCA
ATCTTCCAAATGGGCTCTGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-197)

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V2UF65

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baumannii D1279779

88.48

100

0.885
  pilC Acinetobacter baylyi ADP1

84.069

100

0.841
  pilC Pseudomonas stutzeri DSM 10701

60.688

99.755

0.605
  pilC Legionella pneumophila strain ERS1305867

52.593

99.265

0.522
  pilG Neisseria gonorrhoeae MS11

47.99

97.549

0.468
  pilG Neisseria meningitidis 44/76-A

47.99

97.549

0.468
  pilC Vibrio cholerae strain A1552

43.137

100

0.431
  pilC Vibrio campbellii strain DS40M4

42.822

99.02

0.424
  pilC Thermus thermophilus HB27

36.658

98.284

0.36

Multiple sequence alignment