Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACLKM0_RS12255 Genome accession   NZ_CP183907
Coordinates   2543307..2544167 (+) Length   286 a.a.
NCBI ID   WP_005181774.1    Uniprot ID   -
Organism   Acinetobacter indicus strain M3_NDM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2538307..2549167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM0_RS12235 secG 2538921..2539250 (-) 330 WP_005181783.1 preprotein translocase subunit SecG -
  ACLKM0_RS12240 tpiA 2539264..2540055 (-) 792 WP_411689004.1 triose-phosphate isomerase -
  ACLKM0_RS12245 pilB 2540344..2542050 (+) 1707 WP_160243588.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLKM0_RS12250 pilC 2542081..2543307 (+) 1227 WP_016659656.1 type II secretion system F family protein Machinery gene
  ACLKM0_RS12255 pilD 2543307..2544167 (+) 861 WP_005181774.1 prepilin peptidase Machinery gene
  ACLKM0_RS12260 coaE 2544178..2544786 (+) 609 WP_005181773.1 dephospho-CoA kinase -
  ACLKM0_RS12265 - 2544783..2545703 (-) 921 WP_016659657.1 DMT family transporter -
  ACLKM0_RS12270 rlmB 2545721..2546473 (-) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACLKM0_RS12275 - 2546579..2546902 (-) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32048.33 Da        Isoelectric Point: 7.8299

>NTDB_id=1107138 ACLKM0_RS12255 WP_005181774.1 2543307..2544167(+) (pilD) [Acinetobacter indicus strain M3_NDM]
MQEFIHYLIQHPTALYFVVGIFSLCIGSFLNVVIYRTPKMMEQEWRNDCQMLLHPEQAIIDESKISLSQPASTCPKCHSP
IRWYQNIPVISWLLLRGKCGQCQNPISIRYPFIELLTAVCALTVVAVFGPTLQMLFGLVLTYVLIALTFIDFDTQLLPDR
YTLPLAALGLGVNSYALYTSASSAIWGYIIGFLCLWVVYYLFKLVTGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
VGAIIGIILLKIRKENQPFAFGPYIAIAGWIAFLWGDAIMKLYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=1107138 ACLKM0_RS12255 WP_005181774.1 2543307..2544167(+) (pilD) [Acinetobacter indicus strain M3_NDM]
ATGCAAGAATTCATTCATTATCTGATTCAACACCCAACTGCGCTGTATTTCGTAGTTGGGATTTTTAGTTTATGTATTGG
CAGTTTTCTGAATGTGGTGATTTACCGCACGCCAAAAATGATGGAACAGGAATGGCGCAATGACTGTCAAATGCTGCTGC
ATCCTGAACAGGCCATCATTGATGAAAGTAAAATCAGCCTCAGTCAGCCAGCCTCGACCTGTCCGAAATGCCACAGCCCG
ATTCGCTGGTATCAGAATATTCCAGTAATCAGCTGGCTGCTTCTACGTGGCAAATGTGGCCAATGCCAAAACCCGATCAG
TATTCGTTATCCCTTTATAGAGCTGCTAACCGCAGTGTGTGCCTTAACTGTAGTCGCGGTTTTTGGCCCGACCCTGCAAA
TGCTGTTTGGCCTGGTGTTGACCTATGTGTTAATTGCCCTGACCTTTATTGATTTTGACACCCAGCTCCTGCCTGACCGC
TATACCCTCCCCCTGGCTGCACTCGGCTTGGGGGTGAACAGCTATGCCCTGTACACCTCCGCTAGCTCTGCCATTTGGGG
ATATATCATTGGCTTTTTATGTCTGTGGGTGGTGTATTACCTGTTTAAGCTGGTGACTGGCAAAGAAGGTATGGGCTATG
GCGATTTTAAATTGCTGGCCGCCTTAGGTGCCTGGATGGGCCCGCTGATGCTGCCGCTGATTGTGTTGCTATCTTCTCTG
GTTGGTGCAATCATTGGCATTATTTTGCTGAAAATCCGCAAAGAGAACCAGCCTTTTGCCTTTGGCCCTTATATTGCCAT
TGCCGGCTGGATCGCCTTTTTGTGGGGTGATGCAATCATGAAGCTTTATTTAGGTCAATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(138-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

80

99.65

0.797
  pilD Acinetobacter baumannii D1279779

79.649

99.65

0.794
  pilD Vibrio cholerae strain A1552

55.431

93.357

0.517
  pilD Vibrio campbellii strain DS40M4

53.184

93.357

0.497
  pilD Neisseria gonorrhoeae MS11

47.71

91.608

0.437

Multiple sequence alignment