Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLKM0_RS12245 Genome accession   NZ_CP183907
Coordinates   2540344..2542050 (+) Length   568 a.a.
NCBI ID   WP_160243588.1    Uniprot ID   -
Organism   Acinetobacter indicus strain M3_NDM     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2535344..2547050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM0_RS12205 nusA 2536011..2537495 (-) 1485 WP_005181786.1 transcription termination factor NusA -
  ACLKM0_RS12210 rimP 2537528..2538052 (-) 525 WP_005181784.1 ribosome maturation factor RimP -
  ACLKM0_RS12235 secG 2538921..2539250 (-) 330 WP_005181783.1 preprotein translocase subunit SecG -
  ACLKM0_RS12240 tpiA 2539264..2540055 (-) 792 WP_411689004.1 triose-phosphate isomerase -
  ACLKM0_RS12245 pilB 2540344..2542050 (+) 1707 WP_160243588.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLKM0_RS12250 pilC 2542081..2543307 (+) 1227 WP_016659656.1 type II secretion system F family protein Machinery gene
  ACLKM0_RS12255 pilD 2543307..2544167 (+) 861 WP_005181774.1 prepilin peptidase Machinery gene
  ACLKM0_RS12260 coaE 2544178..2544786 (+) 609 WP_005181773.1 dephospho-CoA kinase -
  ACLKM0_RS12265 - 2544783..2545703 (-) 921 WP_016659657.1 DMT family transporter -
  ACLKM0_RS12270 rlmB 2545721..2546473 (-) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACLKM0_RS12275 - 2546579..2546902 (-) 324 WP_005181767.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63730.11 Da        Isoelectric Point: 4.8254

>NTDB_id=1107136 ACLKM0_RS12245 WP_160243588.1 2540344..2542050(+) (pilB) [Acinetobacter indicus strain M3_NDM]
MSVQQAMPRFTGFIRQLVEEGLVTAENMQQAIVAAKKAETDIVPYLIQQLQISPLTIAEKISYEFGEPIFDLAVYDSRLI
VREGLDEKLFTKYRILPIFRRHNQLYIATSNPTNIEAIDAIRFNSKLNIEVVIVEHDKLERLIEQNFTEDSTFDFDEDFD
LEVGDNAPETQEDNDEPQGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRIDGVLRQIANPPLQLATRLASRL
KVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQG
MLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCQKCKAPVEIPRQSLLELGFTE
EDLNQPEFQLYQPVGCPECREGYKGRVGVYEVMKVTPEISRIIMEDGNALQIAAASAKSGFNNLRRSGLIKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1107136 ACLKM0_RS12245 WP_160243588.1 2540344..2542050(+) (pilB) [Acinetobacter indicus strain M3_NDM]
ATGTCAGTACAACAAGCAATGCCTAGATTTACAGGTTTTATTCGGCAACTGGTCGAAGAAGGGCTTGTGACGGCTGAAAA
TATGCAGCAAGCCATTGTCGCGGCGAAAAAAGCCGAGACCGATATTGTGCCGTATTTAATTCAGCAGTTGCAGATTTCAC
CGCTGACCATTGCTGAAAAAATTTCCTATGAATTTGGTGAACCGATTTTTGACTTGGCGGTGTACGATTCGCGTCTGATT
GTACGTGAAGGTCTAGATGAAAAACTGTTCACCAAATACCGAATTCTGCCCATTTTCCGACGTCACAATCAGCTTTATAT
TGCTACCAGTAACCCAACCAATATCGAAGCCATTGATGCGATCCGCTTTAACAGCAAGCTGAATATTGAAGTGGTGATTG
TTGAGCATGACAAGCTGGAACGGCTGATTGAACAGAACTTTACCGAAGACAGCACCTTTGACTTTGATGAAGACTTTGAT
CTAGAAGTTGGCGACAACGCTCCCGAAACACAAGAAGATAACGATGAGCCACAAGGCGATGAAGCCCCAATCGTCAAATA
TATTAACAAGTTACTGGTCGATGCAATCCGTATGGGCGCCTCGGATTTACACTTCGAGCCTTATGAAAAAAGCTACCGGG
TCCGTTACCGGATTGACGGCGTACTGCGCCAGATTGCCAATCCTCCGTTACAGCTGGCCACACGGCTCGCCTCACGTTTA
AAAGTTATGTCACAAATGGACATCTCGGAAAAACGTATTCCGCAGGATGGCCGGATCAAGCTCAAGCTGTCAAAAAACAA
AGCCATCGACTTCCGTGTCAACTCACTGCCCACCCTGTTCGGGGAAAAAATTGTACTGCGTATTCTCGATCCATCTAGTG
CCATGCTGGGGATTGATGCACTCGGTTATGAACCGGAGCAAAAAGAGCTGTTTATGGAGGCGCTGGACAAACCACAAGGC
ATGTTGCTGATCACCGGCCCGACCGGTTCCGGTAAAACCGTGTCTTTATATACCGGCTTAAACATTCTCAACCGCGAAGA
CACCAATATTTCTACCGCCGAAGATCCGGTGGAAATTAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGAAAGTGG
GTCTGACCTTTGCCGCAGCACTAAAATCATTCCTGCGCCAGGATCCGGATATTGTCATGGTCGGTGAGATCCGTGATCTG
GAAACTGCAGAAATTGCCATTAAAGCCGCACAAACCGGTCACATGGTCATGTCGACGCTGCACACCAACAGTGCACCCGA
AACCCTGACCCGTTTACGCAACATGGGCGTCCCCTCATTTAACATCGCCACCTCGGTGAACCTGGTGATTGCCCAGCGTC
TGGCACGTCGTCTCTGCCAGAAATGCAAGGCCCCGGTGGAGATTCCACGCCAGAGCCTCTTGGAACTCGGTTTTACCGAA
GAAGATTTAAACCAGCCAGAGTTCCAGCTGTATCAGCCGGTTGGCTGCCCGGAATGTCGTGAAGGTTATAAAGGCCGTGT
CGGGGTCTATGAAGTAATGAAAGTCACCCCGGAAATCTCACGCATTATTATGGAAGATGGTAATGCCTTGCAAATTGCAG
CAGCCTCGGCAAAATCCGGGTTTAACAATTTACGCCGTTCAGGATTAATCAAGGTCATGCAGGGGGTCACTTCTTTACAG
GAAGTGAATCGTGTGACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.351

100

0.806
  pilB Acinetobacter baylyi ADP1

77.895

100

0.782
  pilF Neisseria gonorrhoeae MS11

51.964

98.592

0.512
  pilB Legionella pneumophila strain ERS1305867

50.53

99.648

0.504
  pilB Vibrio cholerae strain A1552

49.912

100

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

49.553

98.415

0.488
  pilB Vibrio campbellii strain DS40M4

50.864

91.725

0.467
  pilF Thermus thermophilus HB27

37.258

100

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

33.821

100

0.366

Multiple sequence alignment