Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLKM3_RS12550 Genome accession   NZ_CP183897
Coordinates   2585136..2586362 (+) Length   408 a.a.
NCBI ID   WP_019836082.1    Uniprot ID   A0A1E8E1H7
Organism   Acinetobacter towneri strain M10_NDM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2580136..2591362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM3_RS12510 rimP 2580443..2580967 (-) 525 WP_019836080.1 ribosome maturation factor RimP -
  ACLKM3_RS12535 secG 2581981..2582310 (-) 330 WP_004975868.1 preprotein translocase subunit SecG -
  ACLKM3_RS12540 tpiA 2582324..2583115 (-) 792 WP_019836081.1 triose-phosphate isomerase -
  ACLKM3_RS12545 pilB 2583393..2585105 (+) 1713 WP_004975865.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLKM3_RS12550 pilC 2585136..2586362 (+) 1227 WP_019836082.1 type II secretion system F family protein Machinery gene
  ACLKM3_RS12555 pilD 2586362..2587222 (+) 861 WP_019836083.1 prepilin peptidase Machinery gene
  ACLKM3_RS12560 coaE 2587232..2587831 (+) 600 WP_019836084.1 dephospho-CoA kinase -
  ACLKM3_RS12565 - 2587837..2588715 (-) 879 WP_051092500.1 DMT family transporter -
  ACLKM3_RS12570 rlmB 2588810..2589559 (-) 750 WP_411690844.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACLKM3_RS12575 - 2589748..2590071 (-) 324 WP_019836086.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44980.52 Da        Isoelectric Point: 9.9487

>NTDB_id=1107022 ACLKM3_RS12550 WP_019836082.1 2585136..2586362(+) (pilC) [Acinetobacter towneri strain M10_NDM]
MAVKKAQMMPTFVYEGIDRKGAKLKGEIPARNMALAKVTLRKQGITIKTIRQKKKNILEGLMKKRVSTLDITIFTRQLAT
MMKAGVPLVQSFEIVAEGLENPAMREVVLGIKGEVEGGNTFAGALRKYPQYFDNLFCSLVESGEQSGALETMLDRVAIYK
EKSELLKQKIKKAMKYPATVIVVAVIVTIILMVKVVPVFEELFSSFGADLPAFTKMVVNMSQWMQKYWFILIIVIGVIIA
GFLEAKKRSKKFRDFLDKAALKAPIFGDLVYKAIIARYSRTLATTFAAGVPLIDALESTAGATNNVVYEEAVMKIREDVA
TGQQLQFAMRVTNKFPSMAVQMVAIGEESGALDAMLDKVATHYENEVDNAVDGLTSMMEPLIMSVLGILVGGLVVAMYLP
IFQMGSVV

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=1107022 ACLKM3_RS12550 WP_019836082.1 2585136..2586362(+) (pilC) [Acinetobacter towneri strain M10_NDM]
ATGGCTGTCAAAAAAGCACAGATGATGCCCACCTTCGTTTATGAAGGGATTGACCGCAAAGGGGCTAAGTTAAAAGGGGA
AATTCCAGCGCGTAATATGGCTTTAGCAAAGGTCACATTGCGCAAACAAGGAATTACCATCAAGACGATTCGTCAAAAGA
AAAAGAATATTCTTGAAGGCTTAATGAAAAAACGGGTGTCGACCTTAGACATCACCATTTTCACGCGTCAGCTTGCCACC
ATGATGAAAGCAGGTGTGCCTTTAGTACAAAGCTTTGAGATTGTTGCCGAAGGTCTTGAAAACCCCGCCATGCGTGAAGT
GGTTTTAGGCATTAAAGGTGAAGTAGAAGGCGGGAATACCTTTGCTGGCGCATTACGTAAATATCCTCAATATTTTGACA
ATCTGTTCTGCTCTTTGGTTGAGTCTGGTGAACAATCGGGTGCCCTTGAAACCATGCTAGATCGTGTCGCGATCTATAAA
GAAAAGAGTGAGTTACTCAAACAAAAAATTAAAAAAGCAATGAAATACCCTGCCACGGTCATTGTGGTGGCCGTGATTGT
GACCATTATTTTGATGGTAAAAGTCGTACCCGTATTTGAAGAGTTGTTTAGCTCTTTTGGTGCAGACTTGCCTGCCTTCA
CTAAAATGGTGGTGAACATGTCACAATGGATGCAGAAATACTGGTTTATTCTGATTATTGTGATTGGGGTCATCATCGCA
GGTTTCTTGGAAGCTAAAAAACGCAGTAAAAAATTCCGTGACTTCTTAGACAAAGCGGCACTGAAAGCGCCTATTTTTGG
TGACTTGGTTTATAAAGCCATTATTGCACGTTATAGCCGTACTTTAGCCACTACTTTTGCTGCAGGTGTACCCTTAATTG
ATGCTTTAGAATCCACAGCAGGGGCAACCAATAACGTGGTCTATGAAGAAGCGGTGATGAAAATCCGTGAGGATGTCGCA
ACAGGTCAGCAGCTACAATTTGCCATGCGTGTCACCAATAAATTTCCCTCTATGGCGGTACAAATGGTGGCCATTGGTGA
AGAATCAGGTGCGCTAGATGCCATGCTAGATAAAGTCGCAACCCACTATGAAAACGAAGTCGACAACGCAGTAGATGGTT
TAACCTCAATGATGGAGCCGTTGATTATGTCTGTCTTGGGTATTTTAGTGGGTGGTTTAGTTGTCGCCATGTACCTTCCA
ATCTTCCAAATGGGTTCTGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-400)

Type II secretion system (T2SS), protein F (T2SSF)

(74-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E8E1H7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baumannii D1279779

88.48

100

0.885
  pilC Acinetobacter baylyi ADP1

84.069

100

0.841
  pilC Pseudomonas stutzeri DSM 10701

59.852

99.51

0.596
  pilC Legionella pneumophila strain ERS1305867

53.827

99.265

0.534
  pilG Neisseria gonorrhoeae MS11

47.236

97.549

0.461
  pilG Neisseria meningitidis 44/76-A

46.985

97.549

0.458
  pilC Vibrio cholerae strain A1552

43.627

100

0.436
  pilC Vibrio campbellii strain DS40M4

42.079

99.02

0.417
  pilC Thermus thermophilus HB27

36.79

99.265

0.365

Multiple sequence alignment