Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACLKM3_RS12555 Genome accession   NZ_CP183897
Coordinates   2586362..2587222 (+) Length   286 a.a.
NCBI ID   WP_019836083.1    Uniprot ID   -
Organism   Acinetobacter towneri strain M10_NDM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2581362..2592222
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM3_RS12535 secG 2581981..2582310 (-) 330 WP_004975868.1 preprotein translocase subunit SecG -
  ACLKM3_RS12540 tpiA 2582324..2583115 (-) 792 WP_019836081.1 triose-phosphate isomerase -
  ACLKM3_RS12545 pilB 2583393..2585105 (+) 1713 WP_004975865.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLKM3_RS12550 pilC 2585136..2586362 (+) 1227 WP_019836082.1 type II secretion system F family protein Machinery gene
  ACLKM3_RS12555 pilD 2586362..2587222 (+) 861 WP_019836083.1 prepilin peptidase Machinery gene
  ACLKM3_RS12560 coaE 2587232..2587831 (+) 600 WP_019836084.1 dephospho-CoA kinase -
  ACLKM3_RS12565 - 2587837..2588715 (-) 879 WP_051092500.1 DMT family transporter -
  ACLKM3_RS12570 rlmB 2588810..2589559 (-) 750 WP_411690844.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACLKM3_RS12575 - 2589748..2590071 (-) 324 WP_019836086.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 31995.32 Da        Isoelectric Point: 7.8296

>NTDB_id=1107023 ACLKM3_RS12555 WP_019836083.1 2586362..2587222(+) (pilD) [Acinetobacter towneri strain M10_NDM]
MQELIAYFIQNPTALYIAVGLFSLCIGSFLNVVIYRTPKMMEQEWRNDCQMLLHPEQAIIDESKITLSKPASTCPKCHHP
IHWYQNIPVLSWLVLRGKCGNCQNPISIRYPLVEVLTAVCALVVLAVFGPTLQMLFGLLLTYTLIALTFIDFDTQLLPDR
YTLPLAALGLAVNSYAIYTSPTSAIWGYVIGFLCLWVVYYLFKIVTGKEGMGYGDFKLLAALGAWMGPMMLPLIVLLSSL
VGAIIGIILLKIRKENQPFAFGPYIAIAGWIAFLWGEQIMKVYLGQ

Nucleotide


Download         Length: 861 bp        

>NTDB_id=1107023 ACLKM3_RS12555 WP_019836083.1 2586362..2587222(+) (pilD) [Acinetobacter towneri strain M10_NDM]
ATGCAAGAATTAATCGCGTACTTTATTCAAAATCCAACTGCGCTCTATATCGCAGTTGGACTTTTTAGTTTATGTATTGG
CAGTTTTTTGAATGTGGTAATTTATCGCACCCCAAAAATGATGGAGCAGGAATGGCGTAATGACTGTCAAATGTTGTTAC
ATCCTGAACAAGCCATTATTGATGAAAGTAAAATCACCCTGAGTAAACCTGCATCGACCTGCCCCAAATGTCATCACCCA
ATTCATTGGTATCAAAATATTCCTGTGCTGAGTTGGTTGGTATTACGCGGTAAATGTGGCAACTGCCAAAACCCGATCAG
CATACGTTATCCACTGGTTGAAGTATTAACCGCTGTTTGCGCCTTAGTTGTACTTGCAGTATTTGGCCCAACCTTACAAA
TGCTATTTGGTTTGCTGCTCACTTACACCTTGATTGCACTCACCTTCATTGATTTCGATACACAGCTCTTACCAGACCGT
TATACCCTGCCTTTGGCTGCACTAGGTTTGGCGGTCAACAGCTATGCCATTTATACCTCGCCGACGTCAGCAATTTGGGG
CTATGTAATTGGCTTCCTGTGTCTTTGGGTGGTGTATTACCTGTTTAAAATCGTCACAGGTAAAGAAGGCATGGGCTATG
GTGACTTCAAGCTCCTTGCCGCTTTAGGCGCTTGGATGGGTCCAATGATGTTGCCGCTGATTGTGCTTTTATCTTCTTTG
GTTGGCGCTATTATTGGCATTATTCTGCTAAAAATCCGTAAAGAGAACCAACCTTTTGCATTTGGCCCTTATATTGCAAT
TGCAGGCTGGATTGCCTTTTTATGGGGCGAGCAAATCATGAAAGTTTATTTAGGACAATAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-127)

Type IV leader peptidase family (Peptidase_A24)

(139-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter nosocomialis M2

81.053

99.65

0.808
  pilD Acinetobacter baumannii D1279779

80.702

99.65

0.804
  pilD Vibrio cholerae strain A1552

54.851

93.706

0.514
  pilD Vibrio campbellii strain DS40M4

52.788

94.056

0.497
  pilD Neisseria gonorrhoeae MS11

45.788

95.455

0.437

Multiple sequence alignment