Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLKM3_RS12545 Genome accession   NZ_CP183897
Coordinates   2583393..2585105 (+) Length   570 a.a.
NCBI ID   WP_004975865.1    Uniprot ID   -
Organism   Acinetobacter towneri strain M10_NDM     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2578393..2590105
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKM3_RS12505 nusA 2578923..2580407 (-) 1485 WP_019836079.1 transcription termination factor NusA -
  ACLKM3_RS12510 rimP 2580443..2580967 (-) 525 WP_019836080.1 ribosome maturation factor RimP -
  ACLKM3_RS12535 secG 2581981..2582310 (-) 330 WP_004975868.1 preprotein translocase subunit SecG -
  ACLKM3_RS12540 tpiA 2582324..2583115 (-) 792 WP_019836081.1 triose-phosphate isomerase -
  ACLKM3_RS12545 pilB 2583393..2585105 (+) 1713 WP_004975865.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLKM3_RS12550 pilC 2585136..2586362 (+) 1227 WP_019836082.1 type II secretion system F family protein Machinery gene
  ACLKM3_RS12555 pilD 2586362..2587222 (+) 861 WP_019836083.1 prepilin peptidase Machinery gene
  ACLKM3_RS12560 coaE 2587232..2587831 (+) 600 WP_019836084.1 dephospho-CoA kinase -
  ACLKM3_RS12565 - 2587837..2588715 (-) 879 WP_051092500.1 DMT family transporter -
  ACLKM3_RS12570 rlmB 2588810..2589559 (-) 750 WP_411690844.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACLKM3_RS12575 - 2589748..2590071 (-) 324 WP_019836086.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63472.67 Da        Isoelectric Point: 4.8148

>NTDB_id=1107021 ACLKM3_RS12545 WP_004975865.1 2583393..2585105(+) (pilB) [Acinetobacter towneri strain M10_NDM]
MSVQQATPRFTGFIRRLVEDGLVSAENMQTAIKAAQKDNVDIVPYLIQNLNIAPLIIAEKISNEFGEPIFDLSVYDSALI
VREGIDEKLFSKYCILPIFKRNHLLYVATSNPTNIEAMDAIRFNSKMNIEAIVVEHDKLVKLIEQNFASESAFEFEDDDF
DLNFEDGNSEPEQAAEDEPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKNKSIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFAAALKSFLRQDPDIVMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPHCKAPSDIPKQSLLEMGF
TEADLSNPEFQVYQPVGCSECREGYKGRVGVYEVMKVTPEISRIIMEDGNAIQIADASAKSGFNNLRRSGLLKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1107021 ACLKM3_RS12545 WP_004975865.1 2583393..2585105(+) (pilB) [Acinetobacter towneri strain M10_NDM]
ATGTCAGTACAACAAGCTACACCTAGATTTACAGGTTTTATTCGACGTTTGGTCGAAGATGGGCTCGTTTCTGCTGAAAA
TATGCAAACTGCCATTAAAGCCGCACAAAAAGATAATGTTGATATTGTTCCCTATCTCATTCAAAACCTAAATATCGCGC
CTTTAATCATTGCTGAAAAAATTTCTAATGAGTTTGGCGAACCTATTTTTGATTTAAGCGTGTATGACTCTGCGCTCATC
GTGCGTGAAGGCATTGATGAAAAACTGTTCAGCAAATATTGCATATTGCCTATTTTTAAACGCAATCATCTGCTTTATGT
TGCAACCAGTAACCCGACCAATATCGAAGCAATGGATGCCATTCGTTTCAACAGTAAAATGAATATTGAAGCCATTGTGG
TCGAACACGATAAATTGGTAAAACTGATTGAACAAAACTTTGCGAGTGAGTCTGCTTTTGAATTTGAAGATGATGACTTC
GATCTCAACTTTGAAGATGGTAACAGCGAACCAGAACAAGCTGCAGAAGATGAACCGCAGGGTGATGAATCCCCAATTGT
TAAATACATCAACAAATTACTGATTGATGCAATTCGCATGGGTGCTTCGGACTTACACTTTGAACCGTATGAAAAAACCT
ATCGAGTGCGTTACCGTGTCGATGGTGTATTACGTCAAATTGCCACCCCACCCTTACAGTTAGCTAACCGCTTGGCATCA
CGTTTAAAAGTGATGTCCCAAATGGACATTTCTGAAAAGCGTGTCCCGCAAGATGGTCGTATTAAATTAAAACTATCTAA
AAACAAGTCCATCGACTTCCGTGTGAACTCGTTGCCAACCTTGTTCGGTGAAAAAATCGTACTGCGTATTCTTGACCCAT
CTAGCGCGATGCTTGGGATTGACGCTTTGGGCTATGAACCAGAACAAAAAGAATTGTTCATGGAAGCCTTAGAAAAACCG
CAAGGCATGTTACTGATTACAGGTCCAACGGGTTCGGGTAAAACGGTATCGCTATATACAGGCTTAAATATCTTAAACAG
AGAAGATACCAACATTTCCACTGCCGAAGACCCTGTGGAAATTAACTTAGAAGGCATCAACCAGGTTAACGTCAACAATA
AAGTGGGCTTAACCTTCGCTGCCGCTCTGAAATCTTTCTTACGTCAAGACCCTGATATTGTCATGGTGGGTGAGATTCGT
GACCTTGAAACTGCAGAAATTGCAATTAAAGCAGCCCAAACAGGTCACATGGTTTTATCTACCCTCCACACCAACAGTGC
GCCAGAAACTTTAACGCGTTTACGCAATATGGGCGTTCCTTCCTTTAACATCGCAACTTCGGTAAACCTCGTCATTGCAC
AGCGTTTGGCACGACGCCTATGCCCGCATTGCAAAGCTCCTTCCGATATTCCAAAACAAAGCTTATTGGAAATGGGTTTC
ACTGAAGCCGATTTGAGCAATCCAGAATTTCAGGTCTATCAACCTGTAGGCTGTTCTGAATGCCGTGAAGGTTATAAAGG
ACGTGTTGGTGTTTATGAAGTGATGAAAGTCACACCAGAAATTTCCAGAATTATTATGGAGGATGGTAATGCCATCCAAA
TTGCTGATGCGTCAGCAAAATCGGGGTTTAACAATCTGCGTCGTTCGGGGTTACTTAAGGTCATGCAGGGGGTGACTTCA
TTGCAAGAAGTCAATCGTGTGACCAGTGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-151)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

78.246

100

0.782
  pilB Acinetobacter baylyi ADP1

76.316

100

0.763
  pilB Legionella pneumophila strain ERS1305867

50.345

100

0.512
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio cholerae strain A1552

48.587

99.298

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

48.214

98.246

0.474
  pilB Vibrio campbellii strain DS40M4

50.482

91.053

0.46
  pilF Thermus thermophilus HB27

37.435

100

0.379
  pilB/pilB1 Synechocystis sp. PCC 6803

34.951

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.544

98.596

0.37

Multiple sequence alignment