Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACGHCW_RS02980 Genome accession   NZ_AP031614
Coordinates   599117..600340 (-) Length   407 a.a.
NCBI ID   WP_005446256.1    Uniprot ID   K5TX94
Organism   Vibrio harveyi strain TUMSAT-2019     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 594117..605340
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHCW_RS02940 (VHTUMSATKI_05000) rpsP 594255..594503 (+) 249 WP_004410028.1 30S ribosomal protein S16 -
  ACGHCW_RS02945 (VHTUMSATKI_05010) rimM 594532..595080 (+) 549 WP_005446245.1 ribosome maturation factor RimM -
  ACGHCW_RS02950 (VHTUMSATKI_05020) trmD 595108..595851 (+) 744 WP_005446247.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  ACGHCW_RS02955 (VHTUMSATKI_05030) rplS 595893..596246 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  ACGHCW_RS02960 (VHTUMSATKI_05040) yacG 596535..596729 (-) 195 WP_005446249.1 DNA gyrase inhibitor YacG -
  ACGHCW_RS02965 (VHTUMSATKI_05050) zapD 596792..597532 (-) 741 WP_005446251.1 cell division protein ZapD -
  ACGHCW_RS02970 (VHTUMSATKI_05060) coaE 597560..598174 (-) 615 WP_390502267.1 dephospho-CoA kinase -
  ACGHCW_RS02975 (VHTUMSATKI_05070) pilD 598175..599044 (-) 870 WP_005446255.1 A24 family peptidase Machinery gene
  ACGHCW_RS02980 (VHTUMSATKI_05080) pilC 599117..600340 (-) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  ACGHCW_RS02985 (VHTUMSATKI_05090) pilB 600374..602059 (-) 1686 WP_390502270.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACGHCW_RS02990 (VHTUMSATKI_05100) pilA 602065..602523 (-) 459 WP_017189133.1 pilin Machinery gene
  ACGHCW_RS02995 (VHTUMSATKI_05110) nadC 602788..603675 (-) 888 WP_074050812.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACGHCW_RS03000 (VHTUMSATKI_05120) ampD 603768..604319 (+) 552 WP_390502271.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44945.20 Da        Isoelectric Point: 10.3829

>NTDB_id=110664 ACGHCW_RS02980 WP_005446256.1 599117..600340(-) (pilC) [Vibrio harveyi strain TUMSAT-2019]
MKTIAPQLKNYRWKGINSSGKKTSGNVLAMTEVEVRERLDAQHIKIKKLKKGSISFITKISHRVKGKDITVFTRQIATML
MTGVPIVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASTHFDPLYTDLIATGEQSGNLSQVFERLATYREK
SEQLRAKVIKALIYPAMVVLVALGVSYLMLTKVIPEFEKMFTGFGAELPAFTQMVLNLSAWTQNWGPFIGLSFVSLLISG
KIMSQRSDSFRLLTARSSLRVPILGPVLSKAAIAKFSRTLATSFSAGIPILTALKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYIAMRNCNVFPELVLQMVMIGEESGRLDDMLNKIAAIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=110664 ACGHCW_RS02980 WP_005446256.1 599117..600340(-) (pilC) [Vibrio harveyi strain TUMSAT-2019]
ATGAAAACCATCGCTCCACAACTTAAGAACTACCGTTGGAAAGGCATTAATAGCTCGGGTAAAAAAACCTCTGGTAATGT
GCTCGCAATGACAGAAGTCGAGGTAAGAGAGCGCCTTGATGCTCAGCACATCAAGATAAAAAAACTCAAAAAAGGCAGTA
TTTCTTTTATCACCAAGATAAGTCATCGAGTAAAAGGCAAAGACATTACGGTGTTTACTCGTCAAATCGCCACCATGTTG
ATGACTGGCGTCCCTATTGTTCAGGCACTCAAGTTAGTTTCAGAGAACCATAAAAAAGCTGAAATGAAGTCGATTCTAAT
GAGTGTGACGCGAGCGGTAGAAGCAGGGACACCGATGTCGAAAGCCATGCGTACCGCTAGCACGCACTTTGATCCACTTT
ACACCGACTTAATCGCAACAGGTGAGCAGTCCGGTAACTTATCCCAAGTCTTTGAGCGTCTGGCAACCTACCGAGAAAAA
AGCGAGCAGTTGCGTGCAAAGGTTATTAAAGCGCTCATTTACCCGGCGATGGTGGTATTAGTAGCACTTGGGGTTTCTTA
TTTAATGCTGACAAAGGTTATTCCTGAGTTTGAAAAAATGTTTACAGGTTTTGGTGCCGAATTACCCGCCTTCACCCAGA
TGGTACTCAACCTTTCTGCTTGGACCCAAAATTGGGGTCCCTTCATTGGGCTGAGCTTTGTGAGCTTACTCATTTCTGGC
AAAATCATGTCACAACGCTCCGACTCATTTCGCCTTTTGACTGCACGGTCGAGTTTAAGAGTACCTATTTTAGGACCAGT
TTTATCCAAAGCTGCCATTGCGAAGTTTAGCCGTACTTTGGCGACCAGTTTTAGCGCAGGTATTCCTATCCTAACCGCCT
TGAAAACTACCTCAAAAACCTCTGGTAACATGCACTATCAATTGGCAATCGAAGAGGTATATCGAGATACTGCCGCTGGT
ATGCCAATGTACATTGCCATGCGTAACTGTAACGTCTTCCCAGAATTGGTATTGCAAATGGTCATGATTGGTGAAGAGTC
AGGTCGCTTAGATGACATGCTCAACAAGATAGCCGCTATTTATGAGTTCGAAGTCGATAACACCGTCGACAACCTAAGTA
AGATTTTGGAACCGTTGATCATTGTTTTTCTGGGTGTCGTTGTCGGTGGTTTGGTGACAGCAATGTACTTACCAATCTTT
AACTTGATGAGCGTATTGGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K5TX94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

98.28

100

0.983
  pilC Vibrio cholerae strain A1552

74

98.28

0.727
  pilC Acinetobacter baylyi ADP1

42.462

97.789

0.415
  pilC Pseudomonas stutzeri DSM 10701

41.414

97.297

0.403
  pilC Acinetobacter baumannii D1279779

40.447

99.017

0.4
  pilG Neisseria gonorrhoeae MS11

39.558

100

0.396
  pilC Legionella pneumophila strain ERS1305867

39.163

99.754

0.391
  pilG Neisseria meningitidis 44/76-A

38.725

100

0.388

Multiple sequence alignment