Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACGHCW_RS02985 Genome accession   NZ_AP031614
Coordinates   600374..602059 (-) Length   561 a.a.
NCBI ID   WP_390502270.1    Uniprot ID   -
Organism   Vibrio harveyi strain TUMSAT-2019     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 595374..607059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGHCW_RS02955 (VHTUMSATKI_05030) rplS 595893..596246 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  ACGHCW_RS02960 (VHTUMSATKI_05040) yacG 596535..596729 (-) 195 WP_005446249.1 DNA gyrase inhibitor YacG -
  ACGHCW_RS02965 (VHTUMSATKI_05050) zapD 596792..597532 (-) 741 WP_005446251.1 cell division protein ZapD -
  ACGHCW_RS02970 (VHTUMSATKI_05060) coaE 597560..598174 (-) 615 WP_390502267.1 dephospho-CoA kinase -
  ACGHCW_RS02975 (VHTUMSATKI_05070) pilD 598175..599044 (-) 870 WP_005446255.1 A24 family peptidase Machinery gene
  ACGHCW_RS02980 (VHTUMSATKI_05080) pilC 599117..600340 (-) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  ACGHCW_RS02985 (VHTUMSATKI_05090) pilB 600374..602059 (-) 1686 WP_390502270.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACGHCW_RS02990 (VHTUMSATKI_05100) pilA 602065..602523 (-) 459 WP_017189133.1 pilin Machinery gene
  ACGHCW_RS02995 (VHTUMSATKI_05110) nadC 602788..603675 (-) 888 WP_074050812.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACGHCW_RS03000 (VHTUMSATKI_05120) ampD 603768..604319 (+) 552 WP_390502271.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACGHCW_RS03005 (VHTUMSATKI_05130) pdhR 604724..605491 (+) 768 WP_009699648.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62040.91 Da        Isoelectric Point: 5.2849

>NTDB_id=110665 ACGHCW_RS02985 WP_390502270.1 600374..602059(-) (pilB) [Vibrio harveyi strain TUMSAT-2019]
MLTNLSTVLRQAGILTFSQEESLLEQAKASGISMPEALLISGFFQSHELTEHLSSIFGLSCTSLSQYEYSSLCQKLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFKELTAAIRRLYGRALGQEKSGLKEINQDELAG
LVDIGEDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDHQKQLYLEALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKTEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPHCRLPQEPNSQLQHIGITSTED
IFQANPDGCNECTQGYSGRTGIYEVMKFDESLSNALIKGASVHELEKLAIANGMQTLQMSGIEKLKQGVTSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=110665 ACGHCW_RS02985 WP_390502270.1 600374..602059(-) (pilB) [Vibrio harveyi strain TUMSAT-2019]
ATGCTAACCAACCTCTCAACGGTTCTTCGTCAAGCGGGAATACTGACTTTTTCCCAAGAAGAATCGTTACTTGAGCAAGC
AAAAGCTTCTGGCATTTCGATGCCGGAGGCTTTACTCATTTCTGGCTTTTTTCAATCACATGAATTGACTGAGCACCTTA
GCTCCATCTTTGGGCTTTCCTGCACATCACTCAGTCAGTATGAGTACTCGTCGCTGTGCCAAAAGCTTGGCTTACGCGAT
CTGATCACTCGACATAATGCCTTGCCACTCAATCGCACATCTTCGACATTAATACTCGCGGTTGCAGACCCAACTAACTT
ACAAGCGGAAGATGACTTTCGTTTTGCTACCGGTTTACAAGTCGAGCTAGTTCTGGCGGACTTTAAAGAACTCACCGCAG
CCATTCGTCGTTTATATGGTCGTGCACTCGGTCAAGAAAAGTCCGGTCTTAAAGAAATCAACCAAGATGAACTGGCAGGT
CTCGTTGATATTGGTGAGGACGAAATTGAGAACATTGAAGATCTCAGCCAAGATGAATCGCCTGTTAGTCGTTATATTAA
CCAAATCCTGCTCGATGCCGTTCGTAAAGGGGCTTCTGATATTCACTTCGAGCCCTATGAAAACATGTATCGAGTGCGTT
TGCGTTGCGATGGCATCTTGATCGAAATTCAACAGCCTCCGAGCCATTTAAGCCGTCGCCTCTCAGCCCGAATAAAGATC
CTATCTAAGCTTGATATCGCTGAGCGCCGTTTACCACAGGATGGTCGCATCAAGCTCAAATTAAACCAAGACACCGCGAT
TGATATGCGCGTTTCTACACTGCCAACTCTGTTTGGGGAGAAAATCGTACTGCGTTTGCTCGACAGCAGTTCTGCAGCAT
TAGATATCAATAAACTCGGATACAGCGATCATCAAAAGCAATTGTATTTAGAAGCACTGCGCCGCCCACAAGGCATGATC
CTAATGACAGGACCAACGGGCAGCGGCAAAACGGTTTCTCTTTACACTGGTTTGAGCATTCTCAATAAAACCGAGATCAA
CATTTCCACCGCTGAAGACCCCGTTGAGATCAACTTACCCGGCATTAACCAAGTTCAAGTTCAACCTAAGATCGGCTTTG
GCTTTGCAGAAGCGCTTCGCTCTTTCTTACGTCAAGATCCCGATGTGGTCATGGTGGGTGAGATCCGAGATCTCGACACG
GCAGAAATCGCGATCAAAGCGTCACAAACCGGGCACTTAGTCCTTTCTACCCTGCATACCAACTCCGCCGCCGAGACGGT
CATTCGCTTATCAAATATGGGGGTAGAAAGCTTTAACCTCGCATCCTCTCTTAGTTTGATCATCGCTCAGCGTTTAGCTC
GAAAACTTTGTCCACATTGCAGACTACCTCAAGAACCAAACAGCCAACTTCAACATATTGGAATTACCAGCACTGAAGAT
ATCTTCCAAGCGAACCCTGATGGTTGTAACGAATGCACCCAAGGATACTCTGGCCGCACTGGCATTTACGAAGTGATGAA
GTTTGATGAATCCCTATCAAATGCGCTCATCAAAGGCGCTTCAGTACACGAATTAGAAAAGCTAGCAATTGCCAATGGTA
TGCAGACTCTACAAATGTCCGGCATTGAAAAGCTCAAACAAGGTGTCACCAGCTTTAGTGAGCTGCAACGTGTTCTCTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

96.257

100

0.963
  pilB Vibrio parahaemolyticus RIMD 2210633

89.305

100

0.893
  pilB Vibrio cholerae strain A1552

73.132

100

0.733
  pilB Acinetobacter baylyi ADP1

48.294

99.287

0.479
  pilB Acinetobacter baumannii D1279779

51.838

92.157

0.478
  pilB Legionella pneumophila strain ERS1305867

48.155

96.613

0.465
  pilF Neisseria gonorrhoeae MS11

42.883

100

0.43
  pilB/pilB1 Synechocystis sp. PCC 6803

37.374

100

0.396
  pilF Thermus thermophilus HB27

36.477

100

0.365
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.738

99.465

0.365

Multiple sequence alignment