Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACN08M_RS04605 Genome accession   NZ_CP183250
Coordinates   946112..947215 (-) Length   367 a.a.
NCBI ID   WP_008987965.1    Uniprot ID   A0A0U1P8H5
Organism   Photobacterium leiognathi subsp. mandapamensis strain Sc16.3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 941112..952215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACN08M_RS04575 (ACN08M_04575) - 941416..941901 (+) 486 WP_045064326.1 SprT family zinc-dependent metalloprotease -
  ACN08M_RS04580 (ACN08M_04580) - 942029..942802 (+) 774 WP_419239385.1 endonuclease -
  ACN08M_RS04585 (ACN08M_04585) rsmE 942950..943681 (+) 732 WP_045069248.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ACN08M_RS04590 (ACN08M_04590) gshB 943700..944650 (+) 951 WP_006647266.1 glutathione synthase -
  ACN08M_RS04595 (ACN08M_04595) - 944774..945337 (+) 564 WP_008987967.1 YqgE/AlgH family protein -
  ACN08M_RS04600 (ACN08M_04600) ruvX 945334..945762 (+) 429 WP_023933568.1 Holliday junction resolvase RuvX -
  ACN08M_RS04605 (ACN08M_04605) pilU 946112..947215 (-) 1104 WP_008987965.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACN08M_RS04610 (ACN08M_04610) pilT 947250..948287 (-) 1038 WP_008987964.1 type IV pilus twitching motility protein PilT Machinery gene
  ACN08M_RS04615 (ACN08M_04615) - 948310..949020 (+) 711 WP_419239386.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACN08M_RS04620 (ACN08M_04620) proC 949107..949925 (+) 819 WP_318496359.1 pyrroline-5-carboxylate reductase -
  ACN08M_RS04625 (ACN08M_04625) - 949935..950489 (+) 555 WP_008987961.1 YggT family protein -
  ACN08M_RS04630 (ACN08M_04630) yggU 950486..950779 (+) 294 WP_053987278.1 DUF167 family protein YggU -
  ACN08M_RS04635 (ACN08M_04635) - 950925..951350 (+) 426 WP_008987959.1 DUF4426 domain-containing protein -
  ACN08M_RS04640 (ACN08M_04640) - 951470..952063 (+) 594 WP_045064316.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 41309.47 Da        Isoelectric Point: 6.4411

>NTDB_id=1104843 ACN08M_RS04605 WP_008987965.1 946112..947215(-) (pilU) [Photobacterium leiognathi subsp. mandapamensis strain Sc16.3]
MNLDYILAQMVERKASDLYITVGSPCLLKVDGNLHNLGELLDREAVDALFDEAMDDEQRKVFVTSREANFAIVRNGCRFR
LSAFWQRELPGVVIRRIETKIPNIHDLHLPIDMERLALSKRGLVLVVGATGSGKSTSMAAMTGYRNQNRSGHILTVEDPI
EFIHEHNKCIITQREVGLDTESYEVALKNSLRQAPDMILIGEIRTRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKERRDQFLFDLSLNLKCILAQQLIPDANGIGRHGVYELLLNTPRVADLIRRGELHEIKDVMAKSNQAGMVTFDQSLY
ALFRDGKITEQDALHHADSPNDLRLMIKMGDPNNKTASSLSGVKLDL

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=1104843 ACN08M_RS04605 WP_008987965.1 946112..947215(-) (pilU) [Photobacterium leiognathi subsp. mandapamensis strain Sc16.3]
ATGAATTTAGATTACATCTTAGCGCAGATGGTTGAGCGTAAAGCATCGGATTTATATATCACGGTCGGCTCGCCTTGTTT
GCTTAAAGTCGATGGTAACTTACACAACTTAGGTGAGTTATTAGATCGTGAAGCGGTCGATGCCTTGTTTGATGAAGCGA
TGGATGATGAGCAGCGCAAGGTGTTTGTCACCAGTCGCGAGGCAAACTTTGCGATCGTACGTAACGGTTGTCGTTTTCGC
TTAAGTGCATTTTGGCAACGTGAATTACCGGGTGTGGTGATCCGTCGAATTGAAACCAAGATCCCGAATATTCATGATCT
TCACCTACCTATTGATATGGAGCGTTTAGCGCTATCTAAACGTGGTTTAGTGCTGGTTGTGGGGGCTACGGGGTCGGGTA
AATCGACGTCGATGGCTGCAATGACGGGCTATCGAAACCAAAACCGTAGTGGCCATATTCTAACGGTAGAAGATCCTATT
GAGTTTATTCACGAACATAACAAATGCATCATTACTCAACGTGAAGTGGGACTTGATACTGAAAGTTATGAAGTTGCATT
GAAAAACTCACTGCGCCAAGCACCAGACATGATTTTGATCGGTGAGATCCGTACCCGTGAAACCATGGAATACGCAATGA
CCTTTGCTGAAACAGGTCACTTGTGTATGGCAACACTGCACGCCAACAATGCTAACCAAGCCCTTGAGCGTATTTTGCAT
TTAGTACCTAAAGAGCGTCGAGATCAGTTCTTATTTGATCTGTCATTGAATCTCAAATGTATCTTAGCGCAGCAATTGAT
CCCTGATGCGAATGGTATTGGGCGTCATGGTGTGTATGAGCTATTACTCAATACACCACGTGTTGCTGATTTGATCCGTC
GTGGTGAGCTACATGAAATTAAAGATGTCATGGCGAAATCAAACCAAGCGGGCATGGTGACATTTGACCAATCACTGTAT
GCCTTGTTCCGTGACGGAAAGATCACAGAGCAAGATGCACTGCACCATGCTGACTCACCAAATGATCTGCGTTTGATGAT
TAAAATGGGCGATCCAAACAATAAAACGGCGAGCTCGTTATCCGGCGTTAAATTAGATTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0U1P8H5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

70.572

100

0.706
  pilU Pseudomonas stutzeri DSM 10701

60.112

97.003

0.583
  pilU Acinetobacter baylyi ADP1

55.307

97.548

0.54
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.647

92.643

0.395
  pilT Legionella pneumophila strain Lp02

40.476

91.553

0.371
  pilT Legionella pneumophila strain ERS1305867

40.476

91.553

0.371
  pilT Acinetobacter baumannii D1279779

39.42

94.005

0.371
  pilT Acinetobacter nosocomialis M2

39.42

94.005

0.371
  pilT Acinetobacter baumannii strain A118

39.42

94.005

0.371
  pilT Acinetobacter baylyi ADP1

39.13

94.005

0.368

Multiple sequence alignment