Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACNT2N_RS00220 Genome accession   NZ_CP183197
Coordinates   48657..49874 (+) Length   405 a.a.
NCBI ID   WP_053125326.1    Uniprot ID   -
Organism   Pseudomonas thivervalensis strain NFX106     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 43657..54874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNT2N_RS00200 (ACNT2N_00200) - 45038..45334 (+) 297 WP_053125321.1 DUF2845 domain-containing protein -
  ACNT2N_RS00205 (ACNT2N_00205) - 45669..46022 (-) 354 WP_053125323.1 BON domain-containing protein -
  ACNT2N_RS00210 (ACNT2N_00210) - 46310..46729 (-) 420 WP_053125419.1 pilin -
  ACNT2N_RS00215 (ACNT2N_00215) pilB 46954..48654 (+) 1701 WP_053187360.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNT2N_RS00220 (ACNT2N_00220) pilC 48657..49874 (+) 1218 WP_053125326.1 type II secretion system F family protein Machinery gene
  ACNT2N_RS00225 (ACNT2N_00225) pilD 49876..50745 (+) 870 WP_053125328.1 prepilin peptidase Machinery gene
  ACNT2N_RS00230 (ACNT2N_00230) coaE 50758..51381 (+) 624 WP_053187369.1 dephospho-CoA kinase -
  ACNT2N_RS00235 (ACNT2N_00235) yacG 51378..51584 (+) 207 WP_208665753.1 DNA gyrase inhibitor YacG -
  ACNT2N_RS00240 (ACNT2N_00240) - 51731..51946 (-) 216 WP_053125334.1 hypothetical protein -
  ACNT2N_RS00245 (ACNT2N_00245) - 52010..52696 (-) 687 WP_053125337.1 energy-coupling factor ABC transporter permease -
  ACNT2N_RS00250 (ACNT2N_00250) - 52716..53129 (-) 414 WP_053125339.1 tautomerase family protein -
  ACNT2N_RS00255 (ACNT2N_00255) - 53283..54176 (+) 894 WP_208665754.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44178.91 Da        Isoelectric Point: 10.0551

>NTDB_id=1104437 ACNT2N_RS00220 WP_053125326.1 48657..49874(+) (pilC) [Pseudomonas thivervalensis strain NFX106]
MAVKAVKTDVYTWEGKDRKGAKMTGELTGQSPALVKAQLRKQGINPEKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNPNMRKLVDQVKQEVAAGNSFAASLRKCPQYFDELYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPTAVILVAAVVTGILLVKVVPQFESVFSGFGAQLPAFTVMVIGLSEFMQQSWWLLLGGLAGAFFGVK
YALKRSQAFRDWRDKWLLKLPLIGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIRQDVSTGM
QLNFSMRASGIFPNLAIQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVILGIVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1104437 ACNT2N_RS00220 WP_053125326.1 48657..49874(+) (pilC) [Pseudomonas thivervalensis strain NFX106]
ATGGCGGTCAAGGCAGTAAAAACCGACGTCTATACGTGGGAAGGCAAAGACCGCAAAGGCGCGAAAATGACCGGCGAGTT
GACAGGCCAGAGCCCGGCCCTGGTCAAGGCACAGTTGCGCAAGCAAGGCATCAACCCGGAGAAGGTACGCAAGAAATCCA
CCTCGATATTCAGCAAGGGCAAGCGCATCAAGCCGCTGGATATCGCCCTCTTCACTCGCCAGATGGCGACGATGCTCAAG
GCTGGCGTGCCGCTGTTGCAGGCGTTCGACATCATCGGCGAAGGCTTCGACAACCCCAACATGCGCAAGCTGGTGGATCA
GGTGAAGCAGGAAGTCGCCGCCGGCAACAGCTTCGCCGCCTCGCTGCGCAAATGCCCGCAATATTTCGACGAGCTGTACT
GCAACCTGGTGGACGCCGGCGAACAGGCCGGTGCCCTGGATACGCTGTTGGACCGGGTCGCGACCTACAAGGAAAAGAGC
GAAGCCCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCGACGGCGGTGATACTCGTCGCAGCGGTGGTCACCGGCAT
CCTGTTGGTCAAGGTGGTGCCGCAGTTCGAATCGGTCTTCTCCGGGTTCGGGGCACAGCTGCCGGCCTTCACGGTGATGG
TCATCGGCCTGTCGGAGTTCATGCAGCAATCGTGGTGGTTGCTGCTGGGCGGTTTGGCGGGAGCGTTTTTCGGGGTGAAA
TATGCCCTCAAGCGGTCCCAGGCGTTTCGTGACTGGCGAGACAAGTGGCTGCTCAAGCTCCCCTTGATCGGCACCTTGAT
GTACAAGTCCGCCGTGGCTCGCTTTGCCCGAACGCTCTCGACCACCTTCGCCGCCGGCGTGCCGCTGGTCGAAGCCCTCG
ACTCGGTGTCGGGCGCCACCGGCAACGTGGTGTTCAAGCGCGCCGTGCAGCGCATCCGCCAGGACGTCTCCACCGGCATG
CAGTTGAATTTTTCCATGCGCGCGTCAGGGATCTTTCCGAACCTGGCCATCCAGATGACCGCCATCGGCGAGGAATCCGG
CGCGCTGGACGACATGCTCGACAAGGTCGCGAGCTTTTATGAGGCCGAAGTGGACAATCTGGTGGACAACCTCACCAGCC
TGATGGAACCCTTCATCATGGTGATCCTGGGGATCGTCGTCGGTGGCCTGGTGGTTGCCATGTACCTGCCCATCTTTCAA
CTCGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.827

100

0.738
  pilC Acinetobacter baylyi ADP1

60.302

98.272

0.593
  pilC Acinetobacter baumannii D1279779

57.843

100

0.583
  pilC Legionella pneumophila strain ERS1305867

55.808

97.778

0.546
  pilG Neisseria gonorrhoeae MS11

43.284

99.259

0.43
  pilC Vibrio cholerae strain A1552

43.687

97.778

0.427
  pilG Neisseria meningitidis 44/76-A

42.786

99.259

0.425
  pilC Vibrio campbellii strain DS40M4

43.073

98.025

0.422
  pilC Thermus thermophilus HB27

37.157

99.012

0.368

Multiple sequence alignment