Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACNT2N_RS00215 Genome accession   NZ_CP183197
Coordinates   46954..48654 (+) Length   566 a.a.
NCBI ID   WP_053187360.1    Uniprot ID   -
Organism   Pseudomonas thivervalensis strain NFX106     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 41954..53654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNT2N_RS00195 (ACNT2N_00195) - 42103..45036 (+) 2934 WP_208665752.1 DUF748 domain-containing protein -
  ACNT2N_RS00200 (ACNT2N_00200) - 45038..45334 (+) 297 WP_053125321.1 DUF2845 domain-containing protein -
  ACNT2N_RS00205 (ACNT2N_00205) - 45669..46022 (-) 354 WP_053125323.1 BON domain-containing protein -
  ACNT2N_RS00210 (ACNT2N_00210) - 46310..46729 (-) 420 WP_053125419.1 pilin -
  ACNT2N_RS00215 (ACNT2N_00215) pilB 46954..48654 (+) 1701 WP_053187360.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNT2N_RS00220 (ACNT2N_00220) pilC 48657..49874 (+) 1218 WP_053125326.1 type II secretion system F family protein Machinery gene
  ACNT2N_RS00225 (ACNT2N_00225) pilD 49876..50745 (+) 870 WP_053125328.1 prepilin peptidase Machinery gene
  ACNT2N_RS00230 (ACNT2N_00230) coaE 50758..51381 (+) 624 WP_053187369.1 dephospho-CoA kinase -
  ACNT2N_RS00235 (ACNT2N_00235) yacG 51378..51584 (+) 207 WP_208665753.1 DNA gyrase inhibitor YacG -
  ACNT2N_RS00240 (ACNT2N_00240) - 51731..51946 (-) 216 WP_053125334.1 hypothetical protein -
  ACNT2N_RS00245 (ACNT2N_00245) - 52010..52696 (-) 687 WP_053125337.1 energy-coupling factor ABC transporter permease -
  ACNT2N_RS00250 (ACNT2N_00250) - 52716..53129 (-) 414 WP_053125339.1 tautomerase family protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62696.68 Da        Isoelectric Point: 5.4307

>NTDB_id=1104436 ACNT2N_RS00215 WP_053187360.1 46954..48654(+) (pilB) [Pseudomonas thivervalensis strain NFX106]
MNDIALSGLTKQLVLAELLTEQSAQQAYQQAQRSRIPLVSYLVQNRLVQSRQIAEIASEHFGVALLDLNSLDKEAQPTGL
VSEKLVRQHHALPLWRRGNKLYVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDDTKQDSIGGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKTYRVRVRTDGMLREVARPPIQLATRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCNHCKKAIEIPEETLLKEGFPR
ERIGSFTIYEPVGCEQCNHGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRASGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1104436 ACNT2N_RS00215 WP_053187360.1 46954..48654(+) (pilB) [Pseudomonas thivervalensis strain NFX106]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGCTGGCCGAACTGCTCACCGAGCAAAGTGCGCAACAGGC
GTATCAACAGGCCCAGCGCAGTCGCATCCCGCTGGTCAGCTACCTGGTACAGAACCGGCTGGTGCAAAGCCGTCAGATCG
CGGAAATCGCCTCGGAGCATTTCGGCGTGGCCCTGCTGGACCTCAACAGCCTGGACAAGGAAGCCCAGCCCACCGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCACCACGCCCTGCCGCTGTGGCGACGCGGCAACAAGCTGTACGTGGGCATCTCCGA
CCCGACCAATCACCAGGCCATCAATGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGATGCCATCGAAAAGTTCTTCGAATCCAGTAGCACAGGCCTGGAAGGCATGGGCGATGTCGACCTCGACGGC
CTGGATATCGAATCGATCGACGACACCAAGCAGGACTCCATCGGCGGCCAGGACACCGACGATGCGCCCGTGGTGCGTTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGTTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACTTATCGGG
TGCGGGTGCGCACCGACGGCATGCTGCGAGAAGTGGCCAGGCCGCCGATCCAGCTGGCGACCCGCATCGCCGCCCGCCTG
AAGGTCATGGCCAGCCTCGATATTTCCGAACGGCGCAAGCCCCAGGACGGCCGGCTGAAAATGCGCCTGTCGAAAACCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACGCTTTGGGGCGAAAAGGTAGTGATCCGGATCCTCGACCCGTCCAGCG
CGCAAATGGGCATCGATGCCCTGGGCTACGAGCCGGACCAGAAAGACCTGTACATGGCCGCCCTCAAGCAACCGCAAGGG
CTGATCCTGGTCACCGGCCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACAGGGCTGAATATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGATCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTGAATCCGCGCCAAG
GGCTGGACTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTTTCCACCCTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGCTTGCAGAACATGGGTATCCCGGGGTTCAACATCGCCACCGCCGTGCACCTGATCATCGCCCAGCGGC
TGGCGCGCAAGCTGTGCAACCATTGCAAGAAGGCCATCGAGATTCCCGAGGAAACCTTGCTCAAGGAAGGTTTCCCCCGG
GAACGCATCGGCTCATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACCACGGTTACAAAGGGCGCGTGGGGGT
TTACGAAGTGGTCAAGAACACGCCAGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCACTGGAAATCGATCTGCAGA
TGCGCAAGGACGGCTTCAATGACCTGCGCGCATCGGGGCTGCTCAAAGTGATGCAGGGCGTTACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.481

100

0.585
  pilB Acinetobacter baumannii D1279779

57.27

99.647

0.571
  pilB Legionella pneumophila strain ERS1305867

55.203

100

0.553
  pilB Vibrio cholerae strain A1552

50.442

99.823

0.504
  pilB Vibrio campbellii strain DS40M4

48.596

100

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

49.101

98.233

0.482
  pilF Neisseria gonorrhoeae MS11

48.135

99.47

0.479

Multiple sequence alignment