Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACNH6H_RS06820 Genome accession   NZ_CP182947
Coordinates   1526230..1527450 (+) Length   406 a.a.
NCBI ID   WP_418316725.1    Uniprot ID   -
Organism   Piscinibacter sakaiensis strain 201-F6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1521230..1532450
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNH6H_RS06795 (ACNH6H_06795) rpmA 1522162..1522419 (-) 258 WP_418316719.1 50S ribosomal protein L27 -
  ACNH6H_RS06800 (ACNH6H_06800) rplU 1522459..1522770 (-) 312 WP_418316721.1 50S ribosomal protein L21 -
  ACNH6H_RS06805 (ACNH6H_06805) ispB 1523073..1524014 (+) 942 WP_418321256.1 octaprenyl diphosphate synthase -
  ACNH6H_RS06815 (ACNH6H_06815) pilB 1524366..1526090 (+) 1725 WP_418316723.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNH6H_RS06820 (ACNH6H_06820) pilC 1526230..1527450 (+) 1221 WP_418316725.1 type II secretion system F family protein Machinery gene
  ACNH6H_RS06825 (ACNH6H_06825) pilD 1527465..1528319 (+) 855 WP_418321258.1 prepilin peptidase Machinery gene
  ACNH6H_RS06830 (ACNH6H_06830) coaE 1528330..1528947 (+) 618 WP_418316727.1 dephospho-CoA kinase -
  ACNH6H_RS06835 (ACNH6H_06835) zapD 1529041..1529796 (+) 756 WP_418316729.1 cell division protein ZapD -
  ACNH6H_RS06840 (ACNH6H_06840) - 1529811..1530026 (+) 216 WP_418316731.1 DNA gyrase inhibitor YacG -
  ACNH6H_RS06845 (ACNH6H_06845) - 1530057..1530830 (-) 774 WP_418316733.1 ferredoxin--NADP reductase -
  ACNH6H_RS06850 (ACNH6H_06850) - 1531132..1531422 (+) 291 WP_418316734.1 co-chaperone GroES -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44115.93 Da        Isoelectric Point: 9.7061

>NTDB_id=1103403 ACNH6H_RS06820 WP_418316725.1 1526230..1527450(+) (pilC) [Piscinibacter sakaiensis strain 201-F6]
MATAAAARNIKEIVFEWEGKDKNGKIVRGEMRAGGEAVVSASLRRQGILVSKLKKRRMSGGSAIKQKDIAIFTRQLSTMM
RAGVPLIQSFDIVARGSTNPRMTRLLNDIRSDVETGTSLSAAFRKHPLYFDALYCNLVEAGEAGGILETLLDRLAIYQEK
TMAIKNKIKSALIYPIAVLVVAFVVLSVIMIFVIPAFKEVFSSFGADLPTPTLIVISMSEFFVAYWWAIFGVLGGGVYFF
FQSWKRSEKMQKAMDRLLLRVPVFGDLVKKSAVARWTRTLSTMFAAGVPLVEALDSVGGASGNAVFAEATEKIQRDVSTG
AALTTAMQTTGVFPTMVLQMCAIGEESGALDGMLAKAAEFYEDEVDEAVKGLSSLMEPFIIVILGTLIGGIVVAMYLPIF
KLGQVV

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1103403 ACNH6H_RS06820 WP_418316725.1 1526230..1527450(+) (pilC) [Piscinibacter sakaiensis strain 201-F6]
ATGGCGACTGCAGCGGCCGCACGCAACATCAAGGAAATTGTCTTCGAGTGGGAAGGCAAGGACAAGAACGGCAAGATCGT
TCGCGGCGAGATGCGCGCCGGCGGCGAGGCCGTCGTCAGCGCCAGCCTGCGCCGGCAGGGCATTCTGGTCAGCAAGCTGA
AGAAGCGCCGCATGAGCGGCGGCAGCGCGATCAAGCAGAAGGACATCGCGATCTTCACCCGCCAGCTGTCGACGATGATG
CGCGCCGGCGTGCCGCTGATCCAGTCGTTCGACATCGTCGCCCGCGGCAGCACGAATCCGCGGATGACGCGGCTGCTGAA
CGATATCCGCAGCGATGTCGAGACCGGCACCAGCCTTTCCGCGGCATTTCGCAAGCATCCGCTGTATTTCGACGCGCTGT
ACTGCAACCTGGTCGAAGCCGGTGAAGCCGGCGGCATCCTCGAGACCCTGCTCGATCGCCTGGCGATCTATCAGGAAAAG
ACGATGGCGATCAAGAACAAGATCAAGTCGGCGCTGATCTACCCGATCGCGGTGCTGGTCGTCGCCTTCGTCGTGCTGTC
GGTGATCATGATCTTCGTGATCCCGGCGTTCAAGGAGGTCTTCAGCTCGTTCGGCGCCGACCTGCCGACACCGACGCTGA
TCGTGATCAGCATGTCCGAATTCTTCGTCGCCTATTGGTGGGCGATCTTCGGCGTGCTCGGCGGCGGCGTCTATTTCTTC
TTCCAGTCGTGGAAACGGTCGGAGAAGATGCAGAAGGCGATGGACCGGCTGCTGCTGCGCGTGCCGGTATTCGGCGACCT
GGTCAAGAAGTCTGCGGTGGCGCGCTGGACGCGAACCTTGTCGACGATGTTCGCCGCCGGTGTGCCACTGGTCGAGGCCC
TCGATTCGGTCGGCGGCGCCTCCGGCAATGCCGTCTTCGCCGAGGCGACCGAAAAGATCCAGCGCGATGTGTCGACCGGC
GCTGCGCTGACGACGGCAATGCAGACGACCGGTGTGTTCCCGACGATGGTGCTGCAGATGTGTGCGATCGGCGAGGAATC
CGGCGCGCTCGACGGCATGCTCGCGAAGGCGGCCGAGTTTTATGAGGACGAGGTCGACGAGGCGGTCAAAGGCCTGTCGA
GCCTGATGGAGCCGTTCATCATCGTGATCCTCGGCACACTGATCGGCGGCATCGTCGTCGCAATGTACCTGCCCATCTTC
AAGCTGGGACAGGTGGTGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.198

99.507

0.549
  pilG Neisseria gonorrhoeae MS11

54.66

97.783

0.534
  pilG Neisseria meningitidis 44/76-A

54.408

97.783

0.532
  pilC Legionella pneumophila strain ERS1305867

52.261

98.03

0.512
  pilC Acinetobacter baylyi ADP1

50.251

98.03

0.493
  pilC Acinetobacter baumannii D1279779

49.37

97.783

0.483
  pilC Vibrio cholerae strain A1552

41.919

97.537

0.409
  pilC Vibrio campbellii strain DS40M4

39.801

99.015

0.394
  pilC Thermus thermophilus HB27

38.095

98.276

0.374

Multiple sequence alignment