Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACNH6H_RS06815 Genome accession   NZ_CP182947
Coordinates   1524366..1526090 (+) Length   574 a.a.
NCBI ID   WP_418316723.1    Uniprot ID   -
Organism   Piscinibacter sakaiensis strain 201-F6     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1519366..1531090
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNH6H_RS06785 (ACNH6H_06785) proB 1519858..1520976 (-) 1119 WP_418316715.1 glutamate 5-kinase -
  ACNH6H_RS06790 (ACNH6H_06790) cgtA 1520973..1522067 (-) 1095 WP_418316717.1 Obg family GTPase CgtA -
  ACNH6H_RS06795 (ACNH6H_06795) rpmA 1522162..1522419 (-) 258 WP_418316719.1 50S ribosomal protein L27 -
  ACNH6H_RS06800 (ACNH6H_06800) rplU 1522459..1522770 (-) 312 WP_418316721.1 50S ribosomal protein L21 -
  ACNH6H_RS06805 (ACNH6H_06805) ispB 1523073..1524014 (+) 942 WP_418321256.1 octaprenyl diphosphate synthase -
  ACNH6H_RS06815 (ACNH6H_06815) pilB 1524366..1526090 (+) 1725 WP_418316723.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNH6H_RS06820 (ACNH6H_06820) pilC 1526230..1527450 (+) 1221 WP_418316725.1 type II secretion system F family protein Machinery gene
  ACNH6H_RS06825 (ACNH6H_06825) pilD 1527465..1528319 (+) 855 WP_418321258.1 prepilin peptidase Machinery gene
  ACNH6H_RS06830 (ACNH6H_06830) coaE 1528330..1528947 (+) 618 WP_418316727.1 dephospho-CoA kinase -
  ACNH6H_RS06835 (ACNH6H_06835) zapD 1529041..1529796 (+) 756 WP_418316729.1 cell division protein ZapD -
  ACNH6H_RS06840 (ACNH6H_06840) - 1529811..1530026 (+) 216 WP_418316731.1 DNA gyrase inhibitor YacG -
  ACNH6H_RS06845 (ACNH6H_06845) - 1530057..1530830 (-) 774 WP_418316733.1 ferredoxin--NADP reductase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62491.77 Da        Isoelectric Point: 5.6134

>NTDB_id=1103402 ACNH6H_RS06815 WP_418316723.1 1524366..1526090(+) (pilB) [Piscinibacter sakaiensis strain 201-F6]
MAEMDTLTESAASALSGAARVLVHAGKLAPRVAEEISRKAKEHKSSFVGEVIAAGALTPGELAHTLSSALALPLLDLSTI
SAERLPRNIIDSRMAQQYQVVVLGKRGNRLFIGGADPTDSEAAERIKFATQLTPEWVLVEHDKLSRLVESQTGTAAEALE
ILTSGEFEFDVTEEAPAQEAEATTTEVEDAPVVRFLQKMLIDAINARASDLHFEPYEYHYRVRFRIDGQLREITQPPIAI
KDKLASRIKVISRLDISEKRVPQDGRMKLKFGSKAIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEKIEKERLLT
AVARPYGMILVTGPTGSGKTVSLYTCLNILNQPGVNIATVEDPAEINLPGINQVNVNEKAGLSFATALRAFLRQDPDIIM
VGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVPPFNIASSVLLISAQRLARKLCENCKAVVEYPREAM
IKAGFTEAELDGSWQPYRAVGCSACTNGYKGRVGIYQVMPISDEIQKIILSEGTALDIADQARREGVRDLRQSGLIKVKG
GVTTLEEVISVTNE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1103402 ACNH6H_RS06815 WP_418316723.1 1524366..1526090(+) (pilB) [Piscinibacter sakaiensis strain 201-F6]
ATGGCTGAAATGGATACGCTGACCGAATCAGCAGCTTCAGCCTTGTCCGGTGCTGCCCGGGTTCTCGTTCATGCCGGCAA
GCTCGCACCCCGGGTCGCCGAGGAGATATCGAGAAAGGCGAAGGAACATAAATCGAGCTTCGTCGGCGAAGTGATCGCTG
CCGGCGCGCTGACGCCGGGCGAACTCGCGCACACGCTGTCGAGCGCGCTGGCGCTGCCCTTGCTCGATCTGTCGACGATC
AGTGCCGAGCGACTGCCGCGCAACATCATCGACAGCCGGATGGCGCAGCAGTACCAGGTCGTCGTGCTCGGCAAGCGCGG
CAACCGCCTGTTCATCGGCGGCGCCGACCCGACTGATTCAGAGGCCGCCGAGCGGATCAAGTTCGCGACGCAGCTGACGC
CGGAGTGGGTGCTGGTCGAGCACGACAAGCTGAGCCGCCTGGTCGAGTCGCAGACCGGCACGGCGGCCGAAGCGCTCGAA
ATCCTGACCTCGGGCGAATTCGAGTTCGACGTCACCGAAGAAGCGCCCGCGCAGGAAGCCGAAGCAACGACAACCGAGGT
CGAAGACGCACCGGTGGTCCGCTTTCTGCAGAAGATGCTGATCGACGCGATCAATGCGCGTGCGTCCGACCTGCACTTCG
AACCGTACGAATACCACTACCGCGTCCGGTTCCGGATCGACGGCCAGTTGCGCGAGATCACGCAGCCGCCGATCGCGATC
AAGGACAAGCTCGCATCGCGGATCAAGGTGATCTCCCGGCTCGACATCTCCGAAAAACGGGTTCCGCAAGACGGCCGGAT
GAAGCTGAAGTTCGGCAGCAAGGCGATCGATTTCCGGGTCAGCACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GTATCCTCGATCCCTCGAGCGCCAAGCTCGGCATCGATGCGCTCGGCTACGAGAAGATCGAGAAGGAGCGGCTGCTGACT
GCGGTCGCACGGCCCTACGGCATGATCCTCGTCACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGCTGTACACCTGCCT
GAATATCCTGAACCAACCGGGCGTCAACATCGCGACCGTCGAGGATCCGGCCGAAATCAACCTGCCAGGCATCAACCAGG
TCAATGTCAACGAGAAGGCAGGCCTGAGCTTCGCCACCGCGCTGCGCGCATTCCTGCGGCAGGATCCGGACATCATCATG
GTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCGCGATCAAGGCCGCACAGACCGGACACCTGGTGTTGTCGACGCT
GCACACCAACGATGCACCGACGACGCTGACGCGGCTGATGAACATGGGCGTGCCGCCGTTCAACATCGCGTCGAGCGTGT
TGCTGATTTCCGCGCAGCGGCTGGCCCGCAAGCTGTGTGAAAACTGCAAGGCGGTGGTCGAATATCCGCGCGAGGCGATG
ATCAAGGCCGGTTTCACCGAAGCCGAACTCGACGGCAGCTGGCAGCCCTACAGGGCCGTCGGCTGCTCGGCGTGCACCAA
TGGATACAAGGGCCGCGTCGGCATCTATCAAGTTATGCCGATCAGCGACGAAATCCAGAAGATCATCCTCAGTGAAGGGA
CCGCACTCGACATCGCGGATCAGGCGCGGCGCGAAGGCGTCCGCGACCTTCGGCAATCCGGCCTGATCAAGGTAAAAGGC
GGGGTCACGACGCTCGAGGAAGTGATTTCCGTGACCAACGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-150)

Type II/IV secretion system protein (T2SSE)

(198-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

52.982

99.303

0.526
  pilB Acinetobacter baumannii D1279779

53.286

98.084

0.523
  pilF Neisseria gonorrhoeae MS11

52.669

97.909

0.516
  pilB Legionella pneumophila strain ERS1305867

50.177

98.258

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

46.085

97.909

0.451
  pilB Vibrio campbellii strain DS40M4

45.276

97.735

0.443
  pilB Vibrio cholerae strain A1552

48.654

90.592

0.441
  pilF Thermus thermophilus HB27

38.612

97.909

0.378
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.494

91.638

0.371

Multiple sequence alignment