Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACNANZ_RS05250 Genome accession   NZ_CP181370
Coordinates   1108632..1109849 (-) Length   405 a.a.
NCBI ID   WP_416401657.1    Uniprot ID   -
Organism   Alicycliphilus denitrificans strain A12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1103632..1114849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNANZ_RS05220 (ACNANZ_05220) - 1104721..1105593 (+) 873 WP_094434735.1 ATP-binding protein -
  ACNANZ_RS05225 (ACNANZ_05225) - 1105590..1106015 (+) 426 WP_416401653.1 NUDIX domain-containing protein -
  ACNANZ_RS05230 (ACNANZ_05230) - 1106078..1106284 (-) 207 WP_416401654.1 DNA gyrase inhibitor YacG -
  ACNANZ_RS05235 (ACNANZ_05235) zapD 1106286..1107041 (-) 756 WP_416401655.1 cell division protein ZapD -
  ACNANZ_RS05240 (ACNANZ_05240) coaE 1107119..1107754 (-) 636 WP_416401656.1 dephospho-CoA kinase -
  ACNANZ_RS05245 (ACNANZ_05245) pilD 1107757..1108632 (-) 876 WP_201397468.1 prepilin peptidase Machinery gene
  ACNANZ_RS05250 (ACNANZ_05250) pilC 1108632..1109849 (-) 1218 WP_416401657.1 type II secretion system F family protein Machinery gene
  ACNANZ_RS05255 (ACNANZ_05255) pilB 1109871..1111610 (-) 1740 WP_094434747.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNANZ_RS05260 (ACNANZ_05260) - 1112450..1113454 (+) 1005 WP_416401658.1 hypothetical protein -
  ACNANZ_RS05265 (ACNANZ_05265) - 1114200..1114541 (+) 342 WP_416401659.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44309.06 Da        Isoelectric Point: 9.3572

>NTDB_id=1099871 ACNANZ_RS05250 WP_416401657.1 1108632..1109849(-) (pilC) [Alicycliphilus denitrificans strain A12]
MATIASRDIKDFVFEWEGKDRSGKVVRGEVRASGENQVKATLRRQGVLPTKIKKRRTSGGKKIKPKDIALFTRQMATMMK
AGVPLLQSFDIVGRGNTNASVTKLLNDIRADVETGTSLNAAFRKYPMYFDSLYCNLVEAGEAAGILEALLDRLATYMEKT
EAIKSKIRSALMYPISVLVVAFVVVTVIMIFVIPAFKEVFTSFGADLPAPTLLVMAISEFFVAYWWLIFGAIGGGFYFFM
QAWKRSEKMQRTMDRFLLKMPIFGALIEKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNSVYAVATDRIQQEVSTGT
SLTAAMGNANVFPSMVLQMCAIGEESGSIDHMLGKAADFYEAEVDEMVAGLSSLMEPIIIVFLGTLIGGIVVSMYLPIFK
LGQVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1099871 ACNANZ_RS05250 WP_416401657.1 1108632..1109849(-) (pilC) [Alicycliphilus denitrificans strain A12]
ATGGCGACCATCGCATCAAGGGACATCAAGGACTTCGTCTTTGAGTGGGAGGGCAAGGACCGCAGCGGCAAGGTCGTGCG
TGGCGAGGTCCGGGCCTCCGGCGAGAACCAGGTCAAGGCGACCCTGCGCCGCCAGGGCGTCCTGCCCACCAAGATCAAGA
AGCGCCGGACGAGCGGTGGCAAGAAGATCAAGCCCAAGGACATCGCCCTGTTCACGCGCCAGATGGCCACGATGATGAAG
GCCGGCGTGCCGCTGCTGCAGTCGTTCGACATCGTGGGGCGCGGCAACACCAACGCCAGCGTGACCAAGCTGCTCAACGA
CATCCGCGCCGACGTGGAGACCGGCACCTCGCTCAATGCCGCGTTCCGCAAATATCCCATGTACTTCGACAGCCTGTACT
GCAACCTCGTGGAGGCAGGCGAGGCGGCCGGTATCCTGGAAGCGCTGCTGGACCGCCTGGCCACCTACATGGAGAAGACC
GAGGCCATCAAGTCCAAGATCCGCTCCGCCCTGATGTACCCGATTTCGGTGCTCGTCGTCGCATTCGTCGTGGTCACGGT
GATCATGATCTTCGTGATCCCCGCGTTCAAGGAAGTGTTCACCTCCTTCGGCGCCGACCTGCCCGCGCCCACGTTGCTCG
TGATGGCGATCAGCGAATTCTTCGTCGCCTACTGGTGGCTGATCTTCGGCGCCATCGGGGGGGGCTTCTACTTCTTCATG
CAGGCCTGGAAGCGCAGCGAGAAGATGCAGCGCACCATGGACCGCTTCCTGCTGAAGATGCCCATCTTCGGCGCACTGAT
CGAGAAGTCGTGCGTGGCGCGCTGGACGCGCACCCTGTCGACCATGTTCGCCGCCGGCGTGCCGCTGGTGGAGGCGCTCG
ACTCCGTGGGCGGCGCCTCGGGCAATTCGGTCTATGCCGTGGCCACCGACAGGATCCAGCAGGAGGTCTCCACCGGCACC
AGCCTGACGGCGGCGATGGGCAATGCCAATGTGTTTCCGTCCATGGTGCTGCAGATGTGCGCCATCGGCGAGGAATCGGG
TTCCATCGACCACATGCTGGGCAAGGCGGCCGACTTCTACGAGGCCGAGGTCGACGAGATGGTGGCCGGCCTGTCCAGCC
TCATGGAGCCCATCATCATCGTGTTCCTGGGCACCCTCATCGGCGGCATCGTGGTGTCCATGTACCTGCCCATCTTCAAG
CTGGGGCAAGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.568

100

0.546
  pilG Neisseria gonorrhoeae MS11

53.03

97.778

0.519
  pilG Neisseria meningitidis 44/76-A

52.778

97.778

0.516
  pilC Legionella pneumophila strain ERS1305867

50.758

97.778

0.496
  pilC Acinetobacter baylyi ADP1

49.497

98.272

0.486
  pilC Acinetobacter baumannii D1279779

48.866

98.025

0.479
  pilC Vibrio campbellii strain DS40M4

39.855

100

0.407
  pilC Vibrio cholerae strain A1552

40.657

97.778

0.398
  pilC Thermus thermophilus HB27

36.973

99.506

0.368

Multiple sequence alignment