Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACNANZ_RS05255 Genome accession   NZ_CP181370
Coordinates   1109871..1111610 (-) Length   579 a.a.
NCBI ID   WP_094434747.1    Uniprot ID   A0A3R7HQH8
Organism   Alicycliphilus denitrificans strain A12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1104871..1116610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNANZ_RS05225 (ACNANZ_05225) - 1105590..1106015 (+) 426 WP_416401653.1 NUDIX domain-containing protein -
  ACNANZ_RS05230 (ACNANZ_05230) - 1106078..1106284 (-) 207 WP_416401654.1 DNA gyrase inhibitor YacG -
  ACNANZ_RS05235 (ACNANZ_05235) zapD 1106286..1107041 (-) 756 WP_416401655.1 cell division protein ZapD -
  ACNANZ_RS05240 (ACNANZ_05240) coaE 1107119..1107754 (-) 636 WP_416401656.1 dephospho-CoA kinase -
  ACNANZ_RS05245 (ACNANZ_05245) pilD 1107757..1108632 (-) 876 WP_201397468.1 prepilin peptidase Machinery gene
  ACNANZ_RS05250 (ACNANZ_05250) pilC 1108632..1109849 (-) 1218 WP_416401657.1 type II secretion system F family protein Machinery gene
  ACNANZ_RS05255 (ACNANZ_05255) pilB 1109871..1111610 (-) 1740 WP_094434747.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACNANZ_RS05260 (ACNANZ_05260) - 1112450..1113454 (+) 1005 WP_416401658.1 hypothetical protein -
  ACNANZ_RS05265 (ACNANZ_05265) - 1114200..1114541 (+) 342 WP_416401659.1 helix-turn-helix domain-containing protein -
  ACNANZ_RS05270 (ACNANZ_05270) - 1114541..1115923 (+) 1383 WP_416401660.1 type II toxin-antitoxin system HipA family toxin -
  ACNANZ_RS05275 (ACNANZ_05275) - 1116047..1116493 (-) 447 WP_416401661.1 HAD domain-containing protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 63541.77 Da        Isoelectric Point: 5.4688

>NTDB_id=1099872 ACNANZ_RS05255 WP_094434747.1 1109871..1111610(-) (pilB) [Alicycliphilus denitrificans strain A12]
MASADIAQKVAQPVALPGLARALMSVGKLDQKTAEEIYKKSQTTRTSFIAELTESNAITSSELAHTVSSIFGAPLVDVEA
LDRQRLPKDLLDFKLCQAYRVVPLGKRSNRLIVATADPTNQEAAEKIKFTTQMGVDWIVAEYHKLIQLVDATTKSGSETL
DSIVNSGDFEFGDISVEEASEENSEAAPNEVEDAPVVRFLHKMLLDAFNMRASDLHFEPYEHNYRVRFRIDGELREIASP
PIAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPEEK
ERLLQAISRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQD
PDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPQCKAPADI
PHETLLEAGYKEEEIDGSWVTYRPVGCSACNNGYKGRVGIYQVMPVSEEIQRIILRDGSALDIAEQARLEGVRSLRESGL
HKAKAGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=1099872 ACNANZ_RS05255 WP_094434747.1 1109871..1111610(-) (pilB) [Alicycliphilus denitrificans strain A12]
ATGGCTTCCGCAGACATCGCACAGAAAGTAGCTCAACCCGTCGCGCTACCCGGCCTGGCGCGCGCGCTGATGTCCGTGGG
CAAGCTGGACCAGAAAACCGCCGAGGAAATCTACAAGAAGTCACAAACGACGCGCACCAGCTTCATTGCCGAACTGACCG
AGTCCAACGCGATCACCTCCAGCGAGCTCGCCCACACGGTGTCCTCCATCTTCGGGGCGCCGCTGGTGGATGTGGAGGCG
CTCGACCGCCAGCGCCTGCCGAAGGACCTGCTGGATTTCAAGCTGTGCCAGGCCTACCGGGTGGTTCCCCTGGGCAAGCG
CAGCAACCGCCTGATCGTGGCGACGGCCGATCCGACCAACCAGGAGGCCGCGGAAAAGATCAAGTTCACCACGCAGATGG
GCGTGGACTGGATCGTGGCCGAATACCACAAGCTGATCCAGCTGGTGGACGCGACCACGAAGAGCGGCAGCGAGACCCTT
GACAGCATCGTCAACAGCGGCGACTTCGAGTTTGGCGACATCTCCGTCGAGGAAGCCTCGGAGGAGAACTCGGAAGCCGC
ACCGAACGAGGTCGAGGACGCGCCCGTCGTCAGGTTCCTGCACAAGATGCTGCTGGATGCGTTCAACATGCGCGCATCCG
ACCTGCATTTCGAGCCGTACGAGCACAACTACCGCGTGCGCTTTCGCATCGACGGCGAGCTCAGGGAAATCGCGTCCCCT
CCGATCGCCATCAAGGAGAAGCTGGCATCGCGCATCAAGGTGATCTCCCGCCTGGACATCTCGGAGAAGCGCGTGCCGCA
GGACGGCCGCATGAAGCTCAAGGTGGGGCCGGACCGCGTGATCGATTTCCGCGTGAGCACCCTGCCCACGCTCTTCGGCG
AGAAGATCGTGATCCGTATCCTCGATCCGAGCAGCGCCAAGATGGGCATCGACGCGCTCGGCTACGAACCCGAGGAGAAG
GAGCGCCTGCTCCAGGCCATCAGCCGGCCCTACGGCATGATCCTGGTGACGGGCCCCACGGGGTCGGGCAAGACGGTCTC
GCTCTACACCTGCCTGAACCTGCTGAACAAGCCCGGAGTCAACATCGCCACGGCGGAAGACCCCTCGGAAATCAACCTGC
CGGGCGTGAACCAGGTGAACGTGAACGAAAAGGCGGGGCTGACCTTCGCCACGGCCCTGAAGGCGTTCCTGCGCCAGGAT
CCGGACATCATCATGGTGGGCGAAATCCGCGACCTGGAGACCGCCGACATCTCCATCAAGGCCGCCCAGACGGGCCACCT
GGTGCTGTCCACGCTGCACACCAACGACGCGCCGACCACGCTCACGCGCATGCGCAACATGGGCATCGCGCCCTTCAACA
TCGCATCCAGCGTGATCCTGATCACCGCGCAGCGGCTGGCGCGGCGCCTGTGCCCCCAGTGCAAGGCGCCGGCCGACATA
CCGCACGAGACCCTGCTGGAGGCGGGCTACAAGGAAGAAGAGATCGACGGCTCCTGGGTCACCTACCGGCCCGTCGGCTG
CTCGGCCTGCAACAACGGGTACAAGGGCCGCGTGGGCATCTACCAGGTGATGCCGGTGTCCGAGGAAATCCAGCGCATCA
TCCTGCGCGACGGCAGTGCCCTGGACATCGCCGAGCAGGCCCGCCTGGAGGGCGTGCGCAGCCTGCGCGAGTCGGGGCTG
CACAAGGCGAAGGCGGGATTGACCTCCCTGGAAGAGGTCCTGGCCGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3R7HQH8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.723

97.409

0.523
  pilB Acinetobacter baylyi ADP1

52.265

99.136

0.518
  pilF Neisseria gonorrhoeae MS11

51.943

97.755

0.508
  pilB Legionella pneumophila strain ERS1305867

49.381

97.582

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

46.032

97.927

0.451
  pilB Vibrio cholerae strain A1552

45.234

99.655

0.451
  pilB Vibrio campbellii strain DS40M4

44.779

97.582

0.437
  pilF Thermus thermophilus HB27

40.63

98.618

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.075

92.228

0.37

Multiple sequence alignment