Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMXYT_RS01360 Genome accession   NZ_CP180552
Coordinates   304724..305890 (+) Length   388 a.a.
NCBI ID   WP_415886689.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD37_11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 299724..310890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYT_RS01335 (ACMXYT_01335) metX 299924..301093 (-) 1170 WP_415899875.1 homoserine O-succinyltransferase MetX -
  ACMXYT_RS01340 (ACMXYT_01340) - 301390..301935 (-) 546 WP_415899876.1 YggT family protein -
  ACMXYT_RS01345 (ACMXYT_01345) proC 302034..302861 (-) 828 WP_415899877.1 pyrroline-5-carboxylate reductase -
  ACMXYT_RS01350 (ACMXYT_01350) - 302880..303575 (-) 696 WP_415899878.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYT_RS01355 (ACMXYT_01355) pilT 303667..304701 (+) 1035 WP_415899879.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYT_RS01360 (ACMXYT_01360) pilU 304724..305890 (+) 1167 WP_415886689.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYT_RS01365 (ACMXYT_01365) - 305966..307240 (-) 1275 WP_415899880.1 dihydroorotase -
  ACMXYT_RS01370 (ACMXYT_01370) - 307246..308250 (-) 1005 WP_415899881.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYT_RS01375 (ACMXYT_01375) pyrR 308260..308775 (-) 516 WP_415904961.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYT_RS01380 (ACMXYT_01380) ruvX 308756..309232 (-) 477 WP_415899883.1 Holliday junction resolvase RuvX -
  ACMXYT_RS01385 (ACMXYT_01385) - 309284..309856 (-) 573 WP_415899884.1 YqgE/AlgH family protein -
  ACMXYT_RS01390 (ACMXYT_01390) - 309894..310826 (-) 933 WP_415899885.1 TonB family protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43397.45 Da        Isoelectric Point: 6.0150

>NTDB_id=1096508 ACMXYT_RS01360 WP_415886689.1 304724..305890(+) (pilU) [Neptuniibacter sp. QD37_11]
MDFTQLLKVMVDRDASDLFVTAGARPTIKVDGTLKPLTKDTLKPSQARALVYSTMNDKQLAEFEGCHECNFAISAPGLGR
FRVSAFFQRNSAGMVLRKINNYIPSLEELNLPPILRDLSMTKRGLILFVGGTSTGKSTSLASMIDYRNTNHRGHIITIED
PIEYVHEHKQSIITQREVGIDTDSFDVALKNTLRQAPDVILMGEIRTRETMQHGIVFAETGHLTLATLHANNANQALDRV
ISFFPPEHHDQLWMDLSLNLKAIIAQQLLPTKDGKGRRAAIEVLINTPLIQDLIRKGEVHEIKDVVKKSTNLGMQTFDQS
LFNLYKDGVITYDVALAHADSPNDLRLMIKLSADTNPELAPDEDNQSFHLQDEEDYLHNEGDANVKGM

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=1096508 ACMXYT_RS01360 WP_415886689.1 304724..305890(+) (pilU) [Neptuniibacter sp. QD37_11]
GTGGATTTTACGCAATTACTAAAGGTGATGGTGGATAGGGATGCCTCTGACCTTTTCGTAACGGCAGGTGCAAGGCCGAC
GATTAAGGTTGACGGTACTTTGAAACCGTTAACAAAAGACACCCTTAAGCCATCACAGGCTCGAGCTCTTGTTTACAGTA
CTATGAATGACAAGCAGCTTGCGGAGTTTGAGGGATGTCATGAGTGTAACTTTGCTATTAGTGCGCCAGGTTTGGGACGT
TTTCGTGTATCTGCATTCTTTCAGCGTAACTCTGCAGGCATGGTCTTAAGGAAGATTAATAACTATATACCAAGCTTAGA
AGAGTTGAACCTGCCACCAATCCTACGTGATTTGTCTATGACAAAACGAGGATTAATCTTATTTGTTGGTGGTACCTCTA
CAGGTAAATCAACCTCTTTAGCATCGATGATTGATTACCGTAATACCAATCATCGGGGTCATATTATTACTATCGAAGAT
CCGATTGAGTATGTTCACGAACATAAGCAAAGTATCATCACTCAACGTGAAGTAGGTATCGATACCGACTCGTTTGATGT
AGCACTAAAAAATACGCTCCGTCAGGCACCAGATGTAATTTTGATGGGTGAGATCCGTACTAGGGAAACGATGCAACATG
GTATCGTATTTGCCGAAACAGGTCATTTAACTCTAGCAACTTTACACGCTAATAATGCCAACCAAGCATTAGACCGCGTA
ATTAGCTTCTTCCCGCCTGAGCATCATGATCAGTTGTGGATGGATCTTTCATTAAACCTTAAAGCGATTATTGCGCAACA
ACTGTTACCTACGAAAGATGGTAAGGGACGTCGTGCAGCGATCGAAGTATTGATTAACACGCCATTAATTCAGGATTTGA
TCCGTAAAGGTGAAGTTCATGAGATTAAAGATGTGGTTAAAAAATCAACTAACTTAGGGATGCAGACGTTCGACCAATCT
TTGTTTAATCTCTACAAAGACGGGGTGATTACTTACGATGTGGCACTAGCCCATGCTGATTCGCCAAACGATCTTCGTCT
AATGATTAAGCTGAGTGCTGATACGAATCCTGAATTGGCTCCAGATGAAGATAATCAATCTTTCCATCTACAGGATGAAG
AGGATTACTTGCATAACGAAGGTGATGCTAACGTTAAAGGGATGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.877

92.268

0.626
  pilU Acinetobacter baylyi ADP1

60.833

92.784

0.564
  pilU Vibrio cholerae strain A1552

55.143

90.206

0.497
  pilT Acinetobacter baylyi ADP1

44.737

88.144

0.394
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.058

88.918

0.392
  pilT Acinetobacter nosocomialis M2

43.86

88.144

0.387
  pilT Acinetobacter baumannii D1279779

43.86

88.144

0.387
  pilT Acinetobacter baumannii strain A118

43.86

88.144

0.387
  pilT Pseudomonas stutzeri DSM 10701

42.609

88.918

0.379
  pilT Pseudomonas aeruginosa PAK

42.609

88.918

0.379

Multiple sequence alignment