Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACMXYX_RS01440 Genome accession   NZ_CP180547
Coordinates   335295..336461 (+) Length   388 a.a.
NCBI ID   WP_415897400.1    Uniprot ID   -
Organism   Neptuniibacter sp. QD72_48     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330295..341461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMXYX_RS01415 (ACMXYX_01415) metX 330484..331653 (-) 1170 WP_415906414.1 homoserine O-succinyltransferase MetX -
  ACMXYX_RS01420 (ACMXYX_01420) - 331950..332495 (-) 546 WP_415883219.1 YggT family protein -
  ACMXYX_RS01425 (ACMXYX_01425) proC 332605..333432 (-) 828 WP_415897397.1 pyrroline-5-carboxylate reductase -
  ACMXYX_RS01430 (ACMXYX_01430) - 333451..334146 (-) 696 WP_415897398.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMXYX_RS01435 (ACMXYX_01435) pilT 334238..335272 (+) 1035 WP_415897399.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMXYX_RS01440 (ACMXYX_01440) pilU 335295..336461 (+) 1167 WP_415897400.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACMXYX_RS01445 (ACMXYX_01445) - 336522..337796 (-) 1275 WP_415906415.1 dihydroorotase -
  ACMXYX_RS01450 (ACMXYX_01450) - 337802..338806 (-) 1005 WP_415897402.1 aspartate carbamoyltransferase catalytic subunit -
  ACMXYX_RS01455 (ACMXYX_01455) pyrR 338816..339331 (-) 516 WP_415897403.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMXYX_RS01460 (ACMXYX_01460) ruvX 339312..339788 (-) 477 WP_415883227.1 Holliday junction resolvase RuvX -
  ACMXYX_RS01465 (ACMXYX_01465) - 339843..340415 (-) 573 WP_415883228.1 YqgE/AlgH family protein -
  ACMXYX_RS01470 (ACMXYX_01470) - 340452..341384 (-) 933 WP_415883229.1 TonB family protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 43455.57 Da        Isoelectric Point: 6.0156

>NTDB_id=1096416 ACMXYX_RS01440 WP_415897400.1 335295..336461(+) (pilU) [Neptuniibacter sp. QD72_48]
MDFTQLLKVMVDRDASDLFVTAGARPTIKVDGTLKPLTKDTLKPSQARALVYSTMNDKQLAEFEGCHECNFAISAPGLGR
FRVSAFFQRNSAGMVLRKINNYIPSLEELNLPPILRDLSMTKRGLILFVGGTSTGKSTSLASMIDYRNTNHRGHIITIED
PIEYIHEHKQSIITQREVGIDTDSFDVALKNTLRQAPDVILMGEIRTRETMQHGIVFAETGHLTLATLHANNANQALDRV
ISFFPPEHHDQLWMDLSLNLKAIIAQQLLPTKDGKGRRAAIEVLINTPLIQDLIRKGEVHEIKDVVKKSTNLGMQTFDQS
LFNLYKDGVITYDVALAHADSPNDLRLMIKLSADTNPELAIDEDNQSFHLQDEEDYLHNEGEANIKGM

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=1096416 ACMXYX_RS01440 WP_415897400.1 335295..336461(+) (pilU) [Neptuniibacter sp. QD72_48]
GTGGATTTTACGCAGCTACTAAAGGTCATGGTAGATAGGGATGCCTCTGACCTTTTTGTAACGGCCGGTGCAAGGCCTAC
GATTAAAGTTGATGGTACTTTGAAACCGTTAACTAAAGACACTCTCAAACCTTCACAGGCACGAGCGCTTGTTTATAGCA
CTATGAATGACAAGCAGCTGGCAGAGTTTGAGGGCTGTCATGAATGTAACTTTGCGATTAGCGCTCCAGGTTTAGGACGT
TTTCGTGTATCTGCATTCTTTCAGCGTAACTCAGCGGGTATGGTGCTTAGGAAGATTAATAATTATATCCCGAGTTTAGA
AGAACTGAATTTACCACCGATTCTACGTGATTTGTCTATGACAAAACGAGGGTTGATCTTGTTTGTTGGTGGTACCTCTA
CGGGTAAATCGACTTCCCTAGCATCAATGATTGATTACCGTAATACCAATCACCGCGGTCATATCATTACCATTGAAGAC
CCGATCGAATATATTCACGAGCATAAGCAAAGTATCATCACTCAACGCGAAGTCGGCATAGATACCGACTCGTTTGACGT
CGCCCTAAAAAATACGCTACGTCAGGCACCTGATGTGATTCTGATGGGTGAAATTCGTACTCGGGAAACTATGCAGCACG
GCATTGTATTTGCCGAAACGGGTCATTTAACGTTGGCAACACTACACGCCAATAATGCTAACCAGGCATTGGATCGTGTC
ATCAGTTTCTTCCCGCCTGAGCATCATGATCAGCTATGGATGGATTTGTCGCTGAACTTGAAGGCGATAATCGCGCAACA
ACTTTTACCTACGAAAGATGGTAAGGGGCGTCGTGCAGCGATTGAAGTCTTGATCAACACGCCATTGATTCAGGATTTGA
TTCGTAAGGGTGAAGTTCATGAAATTAAAGATGTGGTTAAGAAATCGACCAACCTTGGGATGCAGACGTTCGATCAATCG
TTGTTTAATCTCTACAAAGACGGGGTGATTACTTACGATGTGGCGCTAGCCCATGCAGATTCGCCAAACGACCTTCGTCT
AATGATTAAGTTGAGCGCTGACACCAACCCAGAACTTGCAATTGATGAAGATAATCAATCATTCCATTTGCAAGATGAAG
AGGATTATCTGCACAACGAGGGTGAAGCAAACATTAAGGGTATGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.156

92.268

0.629
  pilU Acinetobacter baylyi ADP1

61.111

92.784

0.567
  pilU Vibrio cholerae strain A1552

54.857

90.206

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.058

88.918

0.392
  pilT Acinetobacter baylyi ADP1

44.444

88.144

0.392
  pilT Acinetobacter baumannii D1279779

43.567

88.144

0.384
  pilT Acinetobacter baumannii strain A118

43.567

88.144

0.384
  pilT Acinetobacter nosocomialis M2

43.567

88.144

0.384
  pilT Pseudomonas stutzeri DSM 10701

42.319

88.918

0.376
  pilT Pseudomonas aeruginosa PAK

42.319

88.918

0.376

Multiple sequence alignment