Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACMG4M_RS04960 Genome accession   NZ_CP179931
Coordinates   1042422..1043648 (-) Length   408 a.a.
NCBI ID   WP_035249307.1    Uniprot ID   A0A7G2S5P1
Organism   Alcanivorax sp. IL3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1037422..1048648
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMG4M_RS04935 (ACMG4M_04935) argJ 1038121..1039323 (+) 1203 WP_035247993.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  ACMG4M_RS04940 (ACMG4M_04940) - 1039463..1040317 (+) 855 WP_414430799.1 Nudix family hydrolase -
  ACMG4M_RS04945 (ACMG4M_04945) - 1040617..1040808 (-) 192 WP_035249304.1 DNA gyrase inhibitor YacG -
  ACMG4M_RS04950 (ACMG4M_04950) coaE 1040811..1041410 (-) 600 WP_272963346.1 dephospho-CoA kinase -
  ACMG4M_RS04955 (ACMG4M_04955) pilD 1041540..1042418 (-) 879 WP_035249306.1 prepilin peptidase Machinery gene
  ACMG4M_RS04960 (ACMG4M_04960) pilC 1042422..1043648 (-) 1227 WP_035249307.1 type II secretion system F family protein Machinery gene
  ACMG4M_RS04965 (ACMG4M_04965) pilB 1043651..1045357 (-) 1707 WP_035249308.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMG4M_RS04970 (ACMG4M_04970) - 1045943..1046296 (+) 354 WP_272963345.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACMG4M_RS04975 (ACMG4M_04975) - 1046760..1046823 (+) 64 Protein_974 pilus assembly protein PilE -
  ACMG4M_RS04980 (ACMG4M_04980) nadC 1047205..1048044 (-) 840 WP_272963344.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44131.07 Da        Isoelectric Point: 9.4414

>NTDB_id=1093930 ACMG4M_RS04960 WP_035249307.1 1042422..1043648(-) (pilC) [Alcanivorax sp. IL3]
MATKSAEQKNATFVYEGVDRQGKKVKGEASGKGPALVRAELRKQGINAKKVIKKREVSFGGKKKIKPMDIAIFTRQMATM
MKAGVPLVQAFEIVADGLEHPTMRQLVLSLKTEVESGSNFATALSKHPDQFDDLYCSLIEAGEQSGALETMLDRVALYKE
KSELLKQKIKKAMKYPATVVVAAAIVTAILLLKVVPVFQDLFAGFGAELPAFTVFVINLSDGLQKNFLLILACIVAFVIG
FSTLKKKSEPFRDALDRLVLKLPIVGDLAFKSTIARYARTLSTTFAAGVPLIEALDACAGATGNVVYRKAVYKVKEDVST
GQQLQFAMRATGVFPAMALQMTAIGEESGALDAMLEKVAIYYEDEVDNAVDGLTSMMEPLIMSVLGVLIGGLIIAMYLPI
FQLGQVVG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=1093930 ACMG4M_RS04960 WP_035249307.1 1042422..1043648(-) (pilC) [Alcanivorax sp. IL3]
ATGGCAACAAAATCAGCAGAACAAAAAAACGCCACTTTCGTTTATGAAGGTGTTGACAGGCAAGGAAAGAAAGTAAAAGG
CGAGGCTTCTGGCAAAGGTCCAGCTCTGGTTCGTGCAGAACTTCGCAAACAAGGCATTAACGCCAAAAAAGTCATCAAGA
AACGCGAAGTTAGCTTTGGTGGAAAGAAAAAAATCAAACCCATGGATATCGCCATTTTTACTCGTCAGATGGCAACCATG
ATGAAAGCGGGCGTCCCTCTTGTTCAAGCATTTGAAATCGTTGCTGACGGCCTGGAACACCCCACCATGAGACAACTGGT
GCTGAGCCTGAAAACAGAAGTTGAATCAGGCAGTAATTTTGCAACGGCCCTGTCAAAGCATCCAGATCAATTCGACGATT
TGTACTGCAGCTTGATTGAGGCCGGCGAGCAATCAGGCGCGCTTGAAACAATGCTCGATCGAGTCGCGCTTTATAAAGAA
AAAAGCGAACTCCTCAAACAAAAAATCAAGAAAGCCATGAAGTACCCGGCAACGGTTGTTGTTGCGGCTGCCATTGTAAC
TGCAATTCTTTTGCTGAAAGTAGTCCCGGTATTTCAGGATCTTTTCGCCGGTTTCGGTGCTGAACTCCCGGCTTTTACCG
TCTTTGTTATCAATCTGTCAGATGGACTTCAAAAAAACTTTCTGCTTATTCTCGCCTGTATAGTCGCATTTGTAATTGGA
TTTTCCACGCTCAAGAAAAAATCAGAACCCTTCCGGGACGCCCTGGACAGGCTGGTATTAAAACTACCCATTGTCGGTGA
CCTGGCTTTTAAATCGACCATCGCAAGGTACGCACGAACACTCTCAACCACCTTCGCCGCTGGCGTCCCCTTAATCGAGG
CCCTTGATGCTTGTGCCGGGGCGACGGGTAACGTGGTGTATCGCAAAGCCGTTTACAAAGTGAAAGAAGACGTTTCAACT
GGTCAGCAATTGCAATTTGCAATGCGAGCAACAGGTGTCTTCCCCGCCATGGCTCTACAAATGACCGCTATCGGCGAAGA
ATCCGGGGCACTGGATGCCATGCTGGAAAAAGTCGCCATCTATTATGAGGATGAAGTCGACAATGCCGTGGATGGCCTCA
CCAGCATGATGGAGCCTCTCATTATGTCGGTACTTGGCGTTCTTATCGGCGGCTTGATCATCGCCATGTACCTGCCGATT
TTCCAATTGGGTCAAGTTGTAGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(278-399)

Type II secretion system (T2SS), protein F (T2SSF)

(73-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G2S5P1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baumannii D1279779

66.993

100

0.672
  pilC Acinetobacter baylyi ADP1

66.504

100

0.667
  pilC Pseudomonas stutzeri DSM 10701

66.416

97.794

0.65
  pilC Legionella pneumophila strain ERS1305867

52.393

97.304

0.51
  pilG Neisseria gonorrhoeae MS11

44.14

98.284

0.434
  pilC Vibrio cholerae strain A1552

43.276

100

0.434
  pilG Neisseria meningitidis 44/76-A

43.89

98.284

0.431
  pilC Vibrio campbellii strain DS40M4

42.683

100

0.429
  pilC Thermus thermophilus HB27

38.346

97.794

0.375

Multiple sequence alignment