Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMG4M_RS04965 Genome accession   NZ_CP179931
Coordinates   1043651..1045357 (-) Length   568 a.a.
NCBI ID   WP_035249308.1    Uniprot ID   A0A7G2S4F0
Organism   Alcanivorax sp. IL3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1038651..1050357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMG4M_RS04940 (ACMG4M_04940) - 1039463..1040317 (+) 855 WP_414430799.1 Nudix family hydrolase -
  ACMG4M_RS04945 (ACMG4M_04945) - 1040617..1040808 (-) 192 WP_035249304.1 DNA gyrase inhibitor YacG -
  ACMG4M_RS04950 (ACMG4M_04950) coaE 1040811..1041410 (-) 600 WP_272963346.1 dephospho-CoA kinase -
  ACMG4M_RS04955 (ACMG4M_04955) pilD 1041540..1042418 (-) 879 WP_035249306.1 prepilin peptidase Machinery gene
  ACMG4M_RS04960 (ACMG4M_04960) pilC 1042422..1043648 (-) 1227 WP_035249307.1 type II secretion system F family protein Machinery gene
  ACMG4M_RS04965 (ACMG4M_04965) pilB 1043651..1045357 (-) 1707 WP_035249308.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMG4M_RS04970 (ACMG4M_04970) - 1045943..1046296 (+) 354 WP_272963345.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACMG4M_RS04975 (ACMG4M_04975) - 1046760..1046823 (+) 64 Protein_974 pilus assembly protein PilE -
  ACMG4M_RS04980 (ACMG4M_04980) nadC 1047205..1048044 (-) 840 WP_272963344.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACMG4M_RS04985 (ACMG4M_04985) - 1048113..1049453 (-) 1341 WP_414430800.1 alanine/glycine:cation symporter family protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62792.60 Da        Isoelectric Point: 5.5408

>NTDB_id=1093931 ACMG4M_RS04965 WP_035249308.1 1043651..1045357(-) (pilB) [Alcanivorax sp. IL3]
MTSQTLSFSGLPQRLIKQELLTAEDAQQAQQQARQERVSLVHQLVSSGKLSARVVANAVAEEFGDPILDLSAFQRDSLIK
DLVDPKLITKHHVLPLFRRGSRLFVAVSDPTNLPALEEIRFNTGLNVETIIVEEDKLTHWIDHLESEAEGSAFDDLDDDM
GDLDVGPAEEKKDETQSGTDDAPIVRFVNKLLLDAIKGGASDLHFEPYEKTYRVRFRQDGILKTVSKPPINIASKLSARL
KVMSRLDISERRVPQDGRIKLKISKTRAIDFRVNTCPTLFGEKIVLRILDPESAKMGIEALGYDEDQKQMYLDALHKPQG
MILVTGPTGSGKTVSLYTGVNILNTPERNISTAEDPVEINLEGINQVNVNPKQGMDFSAALRAFLRQDPDVILVGEIRDL
ETANIAVKAAQTGHMVLSTLHTNSAPETLTRLRNMGVPSFNIATSVVLIIAQRLARRLCEHCKNVVDVPQQSLLEMGFTE
EDIGTGFTVYGPQGCDKCTNGYKGRVGIYEVVKITDGIARIIMEEGNSIQIADQCRKEGFNDLHRSGLVKVMHGVTSLEE
VNRVTSGH

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1093931 ACMG4M_RS04965 WP_035249308.1 1043651..1045357(-) (pilB) [Alcanivorax sp. IL3]
ATGACTAGCCAGACCCTTTCCTTCAGCGGTTTACCGCAAAGGCTCATCAAGCAGGAATTGCTGACCGCCGAAGACGCACA
GCAAGCGCAACAACAGGCTCGCCAGGAGCGGGTGAGTCTCGTTCACCAGCTGGTAAGCTCCGGCAAGCTCTCGGCGCGAG
TTGTCGCAAATGCAGTGGCCGAAGAGTTTGGCGACCCCATCCTGGATCTGAGCGCCTTTCAGCGTGACAGCCTGATCAAG
GATCTAGTCGATCCAAAACTGATCACCAAGCACCATGTGCTGCCCCTGTTTCGCAGGGGTAGCCGGCTTTTTGTGGCAGT
GTCAGACCCGACCAATTTGCCCGCACTTGAAGAAATCCGTTTCAACACCGGCCTCAATGTAGAAACCATCATCGTTGAGG
AGGACAAGCTCACTCACTGGATTGATCACCTGGAATCAGAAGCCGAAGGCAGCGCTTTTGATGATCTGGATGATGATATG
GGTGATCTTGATGTTGGACCTGCCGAAGAGAAAAAGGACGAAACCCAGTCCGGCACCGATGACGCCCCCATTGTACGCTT
CGTGAACAAGCTGCTGCTGGATGCGATTAAAGGGGGGGCCTCGGATTTACACTTTGAACCTTATGAAAAGACATACCGGG
TGCGGTTCCGTCAGGACGGCATTCTGAAAACCGTCTCCAAGCCACCGATCAACATTGCCAGCAAATTGTCAGCTCGCCTT
AAGGTGATGTCACGACTGGACATTTCTGAACGCCGGGTCCCGCAGGATGGCCGGATCAAGCTGAAAATTTCCAAAACCCG
TGCCATCGATTTCCGGGTAAACACCTGCCCCACGTTGTTCGGGGAAAAAATCGTGTTACGGATTCTGGACCCGGAATCTG
CAAAAATGGGTATTGAGGCGCTGGGCTATGATGAAGATCAAAAACAGATGTATCTGGATGCTCTTCACAAGCCTCAGGGC
ATGATTCTGGTGACTGGCCCCACCGGTTCCGGGAAAACGGTCTCCCTCTATACTGGCGTCAATATCCTCAACACCCCTGA
GCGCAACATTTCTACTGCTGAAGACCCGGTAGAAATCAACCTGGAAGGCATCAATCAGGTTAATGTTAACCCCAAACAGG
GCATGGACTTTTCGGCGGCCCTGCGAGCCTTTCTTCGGCAAGATCCGGATGTCATTCTGGTTGGTGAAATCCGGGATCTG
GAAACCGCCAACATTGCCGTGAAGGCGGCACAGACGGGTCATATGGTGCTTTCTACCTTGCACACCAACAGCGCACCAGA
AACATTGACTCGCCTACGCAACATGGGCGTACCCTCCTTCAATATTGCCACCTCGGTAGTCCTGATTATTGCCCAGCGAT
TAGCACGCCGACTTTGCGAGCACTGCAAAAACGTAGTGGACGTACCGCAACAGTCCCTACTTGAGATGGGCTTCACGGAA
GAAGATATTGGTACCGGGTTTACTGTTTATGGCCCACAAGGGTGCGACAAATGCACTAACGGCTATAAAGGTCGGGTAGG
GATTTATGAAGTCGTCAAAATCACTGACGGTATTGCCCGGATTATTATGGAAGAAGGGAACTCCATCCAAATTGCAGATC
AGTGTCGCAAAGAAGGATTCAATGACTTACATCGCTCCGGCCTCGTAAAAGTCATGCACGGCGTGACCAGCCTGGAAGAA
GTTAACCGTGTAACAAGCGGACATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7G2S4F0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.234

99.296

0.618
  pilB Acinetobacter baylyi ADP1

60.428

98.768

0.597
  pilB Legionella pneumophila strain ERS1305867

54.641

100

0.549
  pilB Vibrio cholerae strain A1552

50.542

97.535

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

98.768

0.493
  pilB Vibrio campbellii strain DS40M4

50.272

97.007

0.488
  pilF Neisseria gonorrhoeae MS11

49.107

98.592

0.484
  pilF Thermus thermophilus HB27

37.975

97.359

0.37
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.238

92.43

0.363

Multiple sequence alignment