Detailed information NTG1093929

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACMG4M_RS04955
• Genome accession: NZ_CP179931
• Coordinates: 1041540..1042418 (-)
• Length: 292 a.a.
• NCBI ID: WP_035249306.1
• Uniprot ID: A0A7G2S4Y2
• Organism: Alcanivorax sp. IL3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1036540..1047418

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMG4M_RS04935 (ACMG4M_04935)	argJ	1038121..1039323 (+)	1203	WP_035247993.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
ACMG4M_RS04940 (ACMG4M_04940)	-	1039463..1040317 (+)	855	WP_414430799.1	Nudix family hydrolase	-
ACMG4M_RS04945 (ACMG4M_04945)	-	1040617..1040808 (-)	192	WP_035249304.1	DNA gyrase inhibitor YacG	-
ACMG4M_RS04950 (ACMG4M_04950)	coaE	1040811..1041410 (-)	600	WP_272963346.1	dephospho-CoA kinase	-
ACMG4M_RS04955 (ACMG4M_04955)	pilD	1041540..1042418 (-)	879	WP_035249306.1	prepilin peptidase	Machinery gene
ACMG4M_RS04960 (ACMG4M_04960)	pilC	1042422..1043648 (-)	1227	WP_035249307.1	type II secretion system F family protein	Machinery gene
ACMG4M_RS04965 (ACMG4M_04965)	pilB	1043651..1045357 (-)	1707	WP_035249308.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACMG4M_RS04970 (ACMG4M_04970)	-	1045943..1046296 (+)	354	WP_272963345.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
ACMG4M_RS04975 (ACMG4M_04975)	-	1046760..1046823 (+)	64	Protein_974	pilus assembly protein PilE	-

Sequence

Protein

• Length: 292 a.a.
• Molecular weight: 31880.80 Da
• Isoelectric Point: 5.7683

>NTDB_id=1093929 ACMG4M_RS04955 WP_035249306.1 1041540..1042418(-) (pilD) [Alcanivorax sp. IL3]
MIDNLFDVLSASPTLLVGLCVFLGLLIGSFLNVVIYRLPVMMERSWQQEAREILELPAKDDGEIFNLVTPRSRCPKCDHG
IAWYENIPVVSWLALKGKCKGCATPISKRYPVIELTSGVLAGLCAWHFGYGPWLAFTLFATWTLLAAAMIDADTTLLPDS
MTYPLLWAGLLAALLGVSPVSLPDAVVGAMAGYLSLWSVYWLFKLLTGKEGMGYGDFKLLAALGAWTGWQYLPLIILLSS
VVGLVFTLIATLFGGEKRTQIPFGPYLAAAGWIALLWGDTIVSSYLGMFKIS

Nucleotide

• Length: 879 bp

>NTDB_id=1093929 ACMG4M_RS04955 WP_035249306.1 1041540..1042418(-) (pilD) [Alcanivorax sp. IL3]
ATGATAGACAATCTTTTCGATGTTCTTTCCGCGAGCCCAACCCTGCTGGTTGGGCTCTGTGTTTTTCTGGGGCTGCTGAT
CGGCAGCTTTCTCAACGTCGTCATCTACCGCTTACCGGTGATGATGGAACGTAGCTGGCAGCAGGAAGCCCGCGAAATTC
TGGAGTTACCCGCAAAGGATGATGGCGAGATATTCAATCTGGTGACACCACGCTCCCGCTGCCCGAAGTGCGACCACGGC
ATTGCCTGGTATGAAAACATCCCGGTGGTGAGCTGGCTGGCACTGAAAGGTAAATGCAAGGGTTGCGCAACGCCGATCAG
TAAACGCTACCCGGTCATTGAGCTGACAAGTGGCGTTCTTGCCGGCCTGTGCGCCTGGCACTTCGGTTATGGACCCTGGC
TAGCCTTTACCCTGTTTGCCACCTGGACCCTGCTGGCGGCAGCCATGATTGATGCGGATACCACCCTGCTGCCGGACAGC
ATGACTTACCCTCTACTGTGGGCCGGCCTGCTGGCGGCGCTGCTTGGCGTTTCACCGGTATCTCTGCCGGATGCGGTGGT
GGGTGCCATGGCCGGTTACCTGTCCCTGTGGAGCGTGTACTGGCTGTTCAAGCTGCTCACCGGCAAGGAAGGCATGGGCT
ATGGTGACTTCAAGCTTCTCGCTGCTCTGGGCGCCTGGACGGGTTGGCAGTATCTGCCGCTGATTATTCTGCTGTCTTCC
GTGGTGGGCCTGGTGTTCACCCTGATCGCCACTCTCTTCGGTGGCGAAAAGCGCACCCAGATCCCTTTCGGCCCCTATCT
AGCGGCCGCTGGCTGGATTGCATTGCTATGGGGCGATACCATCGTCAGCTCGTATCTCGGGATGTTTAAGATCAGTTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (23-128)

• Type IV leader peptidase family (Peptidase_A24) (139-248)

3D structure

• AlphaFold DB: A0A7G2S4Y2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	57.194	95.205	0.545
pilD	Vibrio campbellii strain DS40M4	54.61	96.575	0.527
pilD	Neisseria gonorrhoeae MS11	51.636	94.178	0.486
pilD	Acinetobacter baumannii D1279779	50.725	94.521	0.479
pilD	Acinetobacter nosocomialis M2	50.362	94.521	0.476