Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACK8DD_RS01540 Genome accession   NZ_CP179880
Coordinates   332504..332941 (+) Length   145 a.a.
NCBI ID   WP_000993715.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain MRSN548254     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 327504..337941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8DD_RS01520 (ACK8DD_01520) - 328894..329781 (+) 888 WP_001017320.1 metal-dependent hydrolase -
  ACK8DD_RS01525 (ACK8DD_01525) - 329912..330502 (+) 591 WP_046693071.1 LemA family protein -
  ACK8DD_RS01530 (ACK8DD_01530) - 330524..331606 (+) 1083 WP_001246380.1 TPM domain-containing protein -
  ACK8DD_RS01535 (ACK8DD_01535) - 331600..332160 (+) 561 WP_004839782.1 TPM domain-containing protein -
  ACK8DD_RS01540 (ACK8DD_01540) pilA 332504..332941 (+) 438 WP_000993715.1 pilin Machinery gene
  ACK8DD_RS01545 (ACK8DD_01545) - 332999..334309 (+) 1311 WP_000914627.1 O-antigen ligase family protein -
  ACK8DD_RS01550 (ACK8DD_01550) - 334416..336044 (+) 1629 WP_001195765.1 PglL family O-oligosaccharyltransferase -
  ACK8DD_RS01555 (ACK8DD_01555) bfr 336085..336549 (-) 465 WP_000678123.1 heteropolymeric bacterioferritin subunit Bfr -
  ACK8DD_RS01560 (ACK8DD_01560) - 336794..336988 (-) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  ACK8DD_RS01565 (ACK8DD_01565) - 337198..337581 (-) 384 WP_000090000.1 RidA family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 15022.14 Da        Isoelectric Point: 8.1097

>NTDB_id=1093334 ACK8DD_RS01540 WP_000993715.1 332504..332941(+) (pilA) [Acinetobacter baumannii strain MRSN548254]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIAKSQVSTGLADITAGKTNAETKLAEGLTAALTDVEALGLQKSTNAC
STITTSIGTNGASNITCTLKGTSQINSKKIEWIRDADNATNGTTGAWRCKTDVAENLRPKSCGAS

Nucleotide


Download         Length: 438 bp        

>NTDB_id=1093334 ACK8DD_RS01540 WP_000993715.1 332504..332941(+) (pilA) [Acinetobacter baumannii strain MRSN548254]
ATGAATGCACAAAAAGGTTTTACATTAATCGAACTCATGATCGTAGTTGCCATTATTGGTATTTTGGCTGCGATTGCGAT
TCCTGCTTATCAAAACTACATTGCTAAGTCACAAGTAAGTACTGGTTTAGCTGATATTACTGCTGGTAAGACAAACGCAG
AAACTAAATTAGCAGAAGGTTTAACTGCGGCATTAACTGATGTAGAAGCTTTAGGCTTACAAAAATCTACGAATGCTTGT
AGTACTATTACAACCAGTATCGGAACTAATGGTGCAAGTAATATTACTTGTACATTGAAAGGTACATCACAAATTAATAG
TAAAAAAATTGAATGGATCCGTGATGCAGATAATGCTACAAATGGTACGACAGGTGCTTGGCGCTGTAAAACTGATGTAG
CTGAAAACTTACGTCCTAAATCATGTGGTGCTTCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

52.414

100

0.524
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.218

100

0.448
  pilA Vibrio cholerae strain A1552

44.218

100

0.448
  pilA Vibrio cholerae C6706

44.218

100

0.448
  pilA Pseudomonas aeruginosa PAK

41.558

100

0.441
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.357

98.621

0.428
  comP Acinetobacter baylyi ADP1

40

100

0.414
  pilA Haemophilus influenzae 86-028NP

38.816

100

0.407
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.845

97.931

0.4
  pilA Vibrio parahaemolyticus RIMD 2210633

43.651

86.897

0.379

Multiple sequence alignment