Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLKML_RS14380 Genome accession   NZ_CP178628
Coordinates   1474770..1476017 (-) Length   415 a.a.
NCBI ID   WP_413283770.1    Uniprot ID   -
Organism   Vibrio sp. MA40-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1469770..1481017
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLKML_RS14350 (ACLKML_14350) rplS 1470445..1470798 (+) 354 WP_413283764.1 50S ribosomal protein L19 -
  ACLKML_RS14355 (ACLKML_14355) yacG 1470899..1471093 (-) 195 WP_413283765.1 DNA gyrase inhibitor YacG -
  ACLKML_RS14360 (ACLKML_14360) zapD 1471166..1471906 (-) 741 WP_413283766.1 cell division protein ZapD -
  ACLKML_RS14365 (ACLKML_14365) coaE 1471946..1472551 (-) 606 WP_413283767.1 dephospho-CoA kinase -
  ACLKML_RS14370 (ACLKML_14370) pilD 1472554..1473441 (-) 888 WP_413283768.1 prepilin peptidase Machinery gene
  ACLKML_RS14375 (ACLKML_14375) - 1473475..1474695 (-) 1221 WP_413283769.1 type II secretion system F family protein -
  ACLKML_RS14380 (ACLKML_14380) pilB 1474770..1476017 (-) 1248 WP_413283770.1 GspE/PulE family protein Machinery gene
  ACLKML_RS14385 (ACLKML_14385) - 1476091..1476543 (-) 453 WP_413283771.1 pilin -
  ACLKML_RS14390 (ACLKML_14390) nadC 1476862..1477749 (-) 888 WP_413283772.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACLKML_RS14395 (ACLKML_14395) ampD 1477941..1478498 (+) 558 WP_413283773.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACLKML_RS14400 (ACLKML_14400) pdhR 1478721..1479488 (+) 768 WP_413283774.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 46215.18 Da        Isoelectric Point: 5.8970

>NTDB_id=1090699 ACLKML_RS14380 WP_413283770.1 1474770..1476017(-) (pilB) [Vibrio sp. MA40-2]
MQIQNTISNQQLSEMVTLSDEERITTATHTQSNDPISRYLNQVLTHAIEKKASDIHFEPYEKTYRIRLRCDGVLNEIQSP
DLQLANRLSSKVKVLANLDIAEKRLPQDGRFKFTLISKQTVDIRVSTLPTLWGEKVVLRLLETQTIPLEIDKLGFTSAQQ
DLFLSAIHKPQGLILITGPTGSGKTVSLYTALNIVNTPDKNISTAEDPVEINLLGVNQVQINEKIELNFVITLKALLRQD
PDIIMVGEIRDIDSTEIVIKAAQTGHLVLATLHTNSACETLTRLINMGADKHCLASCLSIIVAQRLVRKLCPICKEIDND
STLALADELQPNNVIYQANNNGCSNCINGYSGRVGLYEILNLDKNIIEAIESQTSAFNIEKIALKNHFLPIKDSGKQKLL
EGITSIDELQRVLLY

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=1090699 ACLKML_RS14380 WP_413283770.1 1474770..1476017(-) (pilB) [Vibrio sp. MA40-2]
ATGCAAATTCAAAATACTATCAGCAACCAACAACTTTCAGAAATGGTCACACTTTCTGATGAAGAACGGATAACAACAGC
CACCCATACTCAAAGTAACGATCCTATCAGCCGTTACTTAAATCAGGTATTAACTCATGCCATCGAAAAAAAAGCTTCAG
ATATTCATTTTGAACCTTACGAAAAAACTTATCGTATTCGATTACGTTGTGATGGTGTTTTAAACGAGATCCAGTCACCC
GATCTTCAATTAGCCAATAGGCTGTCTTCAAAAGTAAAAGTACTTGCCAACCTAGATATAGCGGAAAAACGATTACCTCA
AGATGGTCGATTTAAGTTCACATTAATATCTAAACAAACGGTTGATATTCGAGTGTCTACATTACCCACATTGTGGGGTG
AAAAAGTAGTATTAAGGTTACTAGAAACTCAAACCATTCCCCTAGAAATAGATAAGTTAGGTTTTACTTCGGCTCAACAA
GATCTTTTTTTATCTGCCATACATAAACCTCAAGGGCTTATTTTAATTACTGGCCCTACTGGTAGTGGAAAAACCGTATC
TTTATATACCGCTCTTAATATTGTTAATACTCCCGATAAAAACATTTCAACCGCAGAAGATCCCGTAGAAATAAACCTGT
TAGGTGTTAATCAAGTGCAGATCAATGAAAAAATTGAACTGAATTTTGTTATCACTCTAAAGGCATTATTACGCCAAGAT
CCAGACATCATCATGGTAGGAGAAATTCGAGATATTGATAGTACTGAAATAGTTATTAAAGCAGCACAAACAGGCCACCT
TGTTCTTGCCACCTTACATACCAATTCCGCATGTGAAACACTGACTCGACTCATCAATATGGGAGCAGACAAACACTGTT
TAGCATCATGCTTATCCATTATTGTCGCCCAACGTTTAGTAAGGAAACTATGTCCTATCTGCAAAGAGATCGATAACGAT
TCAACCTTAGCCTTGGCAGACGAATTGCAACCAAATAATGTTATTTATCAAGCAAATAACAATGGCTGTAGTAATTGTAT
AAATGGATACTCAGGCAGAGTCGGACTATATGAAATACTTAACCTCGACAAAAATATAATAGAAGCTATTGAAAGCCAAA
CCAGTGCCTTCAATATTGAGAAAATTGCGTTAAAAAACCATTTCCTTCCTATAAAAGACTCAGGTAAGCAAAAGTTACTA
GAAGGGATAACCAGTATCGATGAATTGCAACGAGTGTTGCTTTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-309)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

55.854

98.795

0.552
  pilB Vibrio parahaemolyticus RIMD 2210633

54.768

98.554

0.54
  pilB Vibrio campbellii strain DS40M4

53.79

98.554

0.53
  pilB Legionella pneumophila strain ERS1305867

51.98

97.349

0.506
  pilB Acinetobacter baumannii D1279779

52.987

92.771

0.492
  pilB Acinetobacter baylyi ADP1

52.727

92.771

0.489
  pilF Neisseria gonorrhoeae MS11

48.705

93.012

0.453
  pilB Haemophilus influenzae Rd KW20

43.932

99.277

0.436
  pilB Haemophilus influenzae 86-028NP

45.619

93.494

0.427
  pilB Glaesserella parasuis strain SC1401

44.125

92.289

0.407
  pilF Thermus thermophilus HB27

42.857

91.084

0.39
  ctsE Campylobacter jejuni subsp. jejuni 81-176

38.702

100

0.388
  pilB/pilB1 Synechocystis sp. PCC 6803

37.531

97.59

0.366

Multiple sequence alignment