Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACL2DY_RS17470 Genome accession   NZ_CP178544
Coordinates   3788522..3789763 (+) Length   413 a.a.
NCBI ID   WP_059113460.1    Uniprot ID   A0AAP3UVF0
Organism   Aeromonas salmonicida strain JK     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3783522..3794763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL2DY_RS17445 (ACL2DY_17445) ampD 3783861..3784433 (-) 573 WP_113720816.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACL2DY_RS17450 (ACL2DY_17450) - 3784583..3785053 (+) 471 WP_408128985.1 TIGR02281 family clan AA aspartic protease -
  ACL2DY_RS17455 (ACL2DY_17455) nadC 3785058..3785915 (+) 858 WP_408135759.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACL2DY_RS17460 (ACL2DY_17460) - 3786251..3786679 (+) 429 WP_353851844.1 pilin -
  ACL2DY_RS17465 (ACL2DY_17465) pilB 3786683..3788389 (+) 1707 WP_408113975.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ACL2DY_RS17470 (ACL2DY_17470) pilC 3788522..3789763 (+) 1242 WP_059113460.1 type II secretion system F family protein Machinery gene
  ACL2DY_RS17475 (ACL2DY_17475) pilD 3789827..3790702 (+) 876 WP_413162875.1 prepilin peptidase Machinery gene
  ACL2DY_RS17480 (ACL2DY_17480) coaE 3790722..3791336 (+) 615 WP_413162877.1 dephospho-CoA kinase -
  ACL2DY_RS17485 (ACL2DY_17485) zapD 3791374..3792096 (+) 723 WP_021140336.1 cell division protein ZapD -
  ACL2DY_RS17490 (ACL2DY_17490) yacG 3792106..3792300 (+) 195 WP_005314167.1 DNA gyrase inhibitor YacG -
  ACL2DY_RS17495 (ACL2DY_17495) mutT 3792378..3792785 (-) 408 WP_005314168.1 8-oxo-dGTP diphosphatase MutT -
  ACL2DY_RS17500 (ACL2DY_17500) - 3792794..3793978 (-) 1185 WP_413162880.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45324.44 Da        Isoelectric Point: 9.8834

>NTDB_id=1090070 ACL2DY_RS17470 WP_059113460.1 3788522..3789763(+) (pilC) [Aeromonas salmonicida strain JK]
MATLTQKQNAPKKVFAFRWSGVNRKGQKVSGELQADSINTVKAELRKQGVNVTRVSKKSQGLFSKGGARIKPMDIAIVSR
QITTMLSAGVPLVQSLQIIARSHEKAAMRELMGQIAADVETGTPMSDALRRHPRHFDALYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPAMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPIFTQFVIGISRFMQNWWYVIFGGIG
LGIFLYVRAWRASQKVKDNTDKFILTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALISAAGASGNYVYRTAVMAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVVA
MYLPIFKLGDVVG

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=1090070 ACL2DY_RS17470 WP_059113460.1 3788522..3789763(+) (pilC) [Aeromonas salmonicida strain JK]
ATGGCAACGCTAACGCAAAAACAGAATGCCCCCAAGAAAGTCTTTGCCTTCCGCTGGAGCGGGGTAAATCGCAAGGGTCA
GAAGGTATCCGGCGAGCTACAGGCCGACAGCATCAATACGGTCAAGGCCGAACTGCGCAAGCAGGGGGTCAATGTCACCA
GAGTGAGCAAAAAGAGCCAGGGGCTTTTTTCCAAGGGTGGCGCCAGGATCAAACCGATGGATATCGCCATCGTTTCCCGT
CAGATCACCACAATGCTCTCCGCCGGGGTACCTCTGGTACAGAGCCTGCAGATCATAGCCCGCAGCCATGAAAAAGCCGC
GATGCGCGAGCTGATGGGCCAGATCGCTGCCGATGTGGAGACAGGCACCCCGATGTCAGATGCCCTGCGCCGCCACCCTC
GCCACTTTGATGCACTCTATTGCGATCTGGTGGAAGCAGGCGAGCAATCCGGTGCCCTGGAAACCATCTACGATCGCATC
GCCACCTATAGAGAAAAGTCGGAAGCGCTCAAGTCCAAGATCAAAAAGGCGATGTTCTATCCCGCCATGGTCATCCTGGT
CGCCATCGTCGTCACCTCCATACTGCTGCTGTTCGTCATCCCGCAGTTCGAGGACATCTTCAAGAGCTTTGGTGCCGAGC
TGCCCATCTTCACCCAGTTTGTCATCGGCATCTCCCGCTTCATGCAGAATTGGTGGTATGTGATTTTTGGCGGAATAGGG
CTTGGCATTTTCCTTTATGTACGAGCGTGGCGCGCGTCCCAGAAGGTCAAAGACAACACAGACAAGTTCATCCTCACCAT
TCCCGTGGTGGGCATGATACTGCACAAGGCGGCCATGGCCCGCTTCGCCCGCACCCTCTCCACCACCTTCTCGGCGGGCA
TACCCCTGGTGGATGCACTGATCTCGGCCGCCGGCGCCTCAGGCAACTATGTCTATCGTACCGCCGTCATGGCCATTCGC
AACGAGGTGGTAGCCGGCATGCAGATCAACGTGGCCATGCGCACCGTGGATCTCTTCCCCGACATGGTGATCCAGATGGT
GATGATCGGTGAGGAGTCAGGCGCCATCGATGATATGCTCTCCAAGGTCGCGACCATCTTCGAGCAAGAGGTCGATGATT
TGGTCGACGGTCTCACCAGCCTGCTGGAGCCTCTCATCATGGTGGTGCTGGGGGTACTGGTCGGCGGCATGGTCGTCGCC
ATGTACCTGCCCATCTTCAAGCTGGGTGACGTGGTAGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.313

95.884

0.54
  pilC Acinetobacter baumannii D1279779

54.5

96.852

0.528
  pilC Acinetobacter baylyi ADP1

52.393

96.126

0.504
  pilC Legionella pneumophila strain ERS1305867

50.617

98.063

0.496
  pilC Vibrio cholerae strain A1552

47.382

97.094

0.46
  pilC Vibrio campbellii strain DS40M4

46.535

97.821

0.455
  pilG Neisseria gonorrhoeae MS11

40.247

98.063

0.395
  pilG Neisseria meningitidis 44/76-A

40

98.063

0.392
  pilC Thermus thermophilus HB27

37.75

96.852

0.366

Multiple sequence alignment