Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACL2DY_RS17465 Genome accession   NZ_CP178544
Coordinates   3786683..3788389 (+) Length   568 a.a.
NCBI ID   WP_408113975.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain JK     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3781683..3793389
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL2DY_RS17440 (ACL2DY_17440) pdhR 3782820..3783584 (-) 765 WP_005314150.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ACL2DY_RS17445 (ACL2DY_17445) ampD 3783861..3784433 (-) 573 WP_113720816.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACL2DY_RS17450 (ACL2DY_17450) - 3784583..3785053 (+) 471 WP_408128985.1 TIGR02281 family clan AA aspartic protease -
  ACL2DY_RS17455 (ACL2DY_17455) nadC 3785058..3785915 (+) 858 WP_408135759.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACL2DY_RS17460 (ACL2DY_17460) - 3786251..3786679 (+) 429 WP_353851844.1 pilin -
  ACL2DY_RS17465 (ACL2DY_17465) pilB 3786683..3788389 (+) 1707 WP_408113975.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ACL2DY_RS17470 (ACL2DY_17470) pilC 3788522..3789763 (+) 1242 WP_059113460.1 type II secretion system F family protein Machinery gene
  ACL2DY_RS17475 (ACL2DY_17475) pilD 3789827..3790702 (+) 876 WP_413162875.1 prepilin peptidase Machinery gene
  ACL2DY_RS17480 (ACL2DY_17480) coaE 3790722..3791336 (+) 615 WP_413162877.1 dephospho-CoA kinase -
  ACL2DY_RS17485 (ACL2DY_17485) zapD 3791374..3792096 (+) 723 WP_021140336.1 cell division protein ZapD -
  ACL2DY_RS17490 (ACL2DY_17490) yacG 3792106..3792300 (+) 195 WP_005314167.1 DNA gyrase inhibitor YacG -
  ACL2DY_RS17495 (ACL2DY_17495) mutT 3792378..3792785 (-) 408 WP_005314168.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62546.97 Da        Isoelectric Point: 5.4384

>NTDB_id=1090069 ACL2DY_RS17465 WP_408113975.1 3786683..3788389(+) (pilB) [Aeromonas salmonicida strain JK]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESEQDALGMEDIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCEHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1090069 ACL2DY_RS17465 WP_408113975.1 3786683..3788389(+) (pilB) [Aeromonas salmonicida strain JK]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
CCTGAGCCAAGCCAAGGCCCAGCGCAAACCCTTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTTTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAACTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTTGGGCTCCATACCGAAGCTCTGCTGGTGGAAGAGAACA
AGCTTACCGCCGCCATCGGCAAGCTGCTGGAAAGCGAGCAGGATGCCCTCGGCATGGAGGATATCGACGAGTCGGAGATC
TCCGAGCTGGAAGTCTCGGACGAAAACTCCCGCCTCGATGAGAGCGTCAATACCGCAGATGACGATGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGCGCATCCGACCTGCACTTCGAACCCTATGAGACCAAGTACC
GTATCCGCTTTCGTATAGACGGCATACTGCACGAGATAGCGACGCCGCCGGTCAATTTGGCCAACCGCTTTTCGGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCCGAGCGACGGCTGCCCCAGGATGGTCGCATCAAGCTCAAGCTGTCGCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCAG
CGGCGCGGCTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAGGCACAGTATCTGCGGGCCCTCTCCAAGCCCCAG
GGCATGATACTGGTGACAGGCCCGACCGGTTCTGGCAAAACTGTCTCTCTCTATACCGGCCTCAATATTCTCAACACCAC
AGAGGTCAACATCTCTACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAGGTACAAGTCAATCCCAAGG
CGGGGCTGACCTTCGCCAGCGCCCTGCGCTCCTTTCTTCGCCAGGATCCCGATGTGGTGATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAAGCCGCCCAGACCGGCCACCTGGTACTCTCCACCCTGCACACCAACTCGGCTGC
CGAAACCCTGACTCGAATGATGAACATGGGGGTCCCGGCCTTCAACATCGCCTCTTCGGTAACCCTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGAGCACTGCAAGGCACCGGAAGTGGTGCCCGAGGCCGAACTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAACCGGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATCATGCAAGGAGCCAACTCCCTGCAAATTGCAG
CCATTGCTCAAAAAGAGGGGATGCGAACCCTGCGTGTCTCGGGCCTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAGATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Acinetobacter baumannii D1279779

55.458

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.69

99.472

0.544
  pilB Vibrio cholerae strain A1552

54.336

99.472

0.54
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.418

99.296

0.511
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.53

92.958

0.377

Multiple sequence alignment