Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACLRDL_RS00410 Genome accession   NZ_CP178387
Coordinates   68928..70643 (-) Length   571 a.a.
NCBI ID   WP_000788426.1    Uniprot ID   A0A0K9UP29
Organism   Vibrio cholerae isolate CTMA_1842     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 63928..75643
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLRDL_RS00390 (ACLRDL_00390) - 64671..65504 (-) 834 WP_000744679.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ACLRDL_RS00395 (ACLRDL_00395) - 65574..67049 (-) 1476 WP_032477105.1 AAA family ATPase -
  ACLRDL_RS00400 (ACLRDL_00400) aroB 67104..68189 (-) 1086 WP_000439805.1 3-dehydroquinate synthase -
  ACLRDL_RS00405 (ACLRDL_00405) aroK 68217..68741 (-) 525 WP_000818616.1 shikimate kinase AroK -
  ACLRDL_RS00410 (ACLRDL_00410) pilQ 68928..70643 (-) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  ACLRDL_RS00415 (ACLRDL_00415) pilP 70669..71187 (-) 519 WP_000792033.1 pilus assembly protein PilP Machinery gene
  ACLRDL_RS00420 (ACLRDL_00420) pilO 71177..71767 (-) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACLRDL_RS00425 (ACLRDL_00425) pilN 71760..72344 (-) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  ACLRDL_RS00430 (ACLRDL_00430) pilM 72334..73350 (-) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62340.49 Da        Isoelectric Point: 4.6119

>NTDB_id=1089365 ACLRDL_RS00410 WP_000788426.1 68928..70643(-) (pilQ) [Vibrio cholerae isolate CTMA_1842]
MKNGLKTYVAQTWLTLWVGLALCASSMVFSAESATANQLENIDFRVNKEKAAVLIVELASPSAVVDVQKVQEGLSIELLK
TDVADDKLYLLDVKDFSTPVESVEVFRKEPSTQLVVTVDGEFQHDYTLKGKYLEVVISKLKADEKPKPKSVLEKEGKLIS
INFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILIAPKEELDLREKQAL
EKARLAEELGDLKSEIIKINFAKASDIAAMIGGEGNVNMLSERGSISIDERTNSLLIRELPDNIAVIREIIESLDIPVKQ
VQIEARIVTVKEGNLEELGVRWGVMSTNGSHSVGGSIESNLWQKGLLADDEFPVDEFLNVNLASTSANASSIAFQVAKLG
SGTLLDLELSALQNESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGASTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRRGETVKTGTGEAVSIDTQRIGTQVLVNNGETVVLGGIFQHSINNSVDKVPLLGDLPVLGALFRRTYEQMGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1089365 ACLRDL_RS00410 WP_000788426.1 68928..70643(-) (pilQ) [Vibrio cholerae isolate CTMA_1842]
ATGAAAAATGGATTGAAAACTTATGTCGCACAGACTTGGCTGACCTTATGGGTTGGTTTGGCACTTTGTGCTTCTTCCAT
GGTGTTTAGTGCTGAATCCGCTACCGCCAACCAGTTGGAAAACATCGACTTTCGGGTCAACAAAGAGAAGGCGGCGGTCT
TAATTGTCGAATTGGCTTCGCCATCGGCGGTCGTGGATGTGCAAAAAGTCCAAGAAGGGTTGAGCATTGAACTCCTGAAA
ACCGATGTGGCGGATGACAAGCTGTATCTGCTCGATGTGAAAGATTTTTCGACCCCTGTTGAGAGCGTGGAAGTATTCCG
TAAAGAGCCAAGCACACAACTTGTTGTGACTGTCGATGGGGAGTTTCAGCACGATTATACCTTGAAAGGAAAATACCTTG
AGGTCGTGATCAGTAAACTCAAAGCCGATGAAAAGCCTAAGCCCAAGAGTGTCTTGGAAAAAGAAGGCAAGCTGATTTCA
ATTAACTTCCAAGATATTCCGGTGCGTAACGTACTGCAGCTGATTGCCGATTATAACGGTTTCAACTTAGTGGTTTCCGA
TTCGGTAGTGGGTAACCTGACTTTACGCTTGGATGGCGTGCCTTGGCAGCAAGTGTTGGACATTATTCTGCAAGTCAAAG
GGCTCGATAAGCGGGTCGATGGCAATGTGATTTTGATTGCGCCGAAAGAGGAACTCGACTTGCGCGAGAAACAAGCGCTC
GAAAAAGCCCGTTTAGCAGAAGAGTTGGGTGATTTAAAATCAGAAATCATCAAGATTAACTTTGCCAAAGCGTCTGACAT
TGCGGCGATGATCGGCGGTGAAGGCAACGTTAACATGTTGTCTGAACGAGGTTCGATCAGCATCGACGAACGAACTAACT
CGCTATTGATCCGAGAGCTGCCCGATAACATTGCTGTGATCCGCGAGATCATTGAATCGCTGGATATTCCGGTCAAACAA
GTTCAAATCGAAGCGCGGATTGTCACCGTCAAAGAAGGTAACTTGGAGGAGCTGGGGGTACGCTGGGGCGTAATGTCGAC
CAATGGTAGCCACTCGGTCGGAGGAAGCATAGAGAGTAACTTATGGCAAAAAGGGTTATTGGCGGATGATGAATTTCCGG
TGGATGAATTCTTAAATGTGAATTTGGCTTCCACCTCTGCCAATGCGTCTTCGATTGCTTTCCAAGTCGCTAAATTGGGC
TCGGGTACATTGTTGGATTTGGAGCTTTCAGCATTACAAAATGAATCGAAAGCCGAAATCATCTCTAGCCCTCGCTTGAT
TACTACCAATAAACAGCCTGCCTATATTGAGCAAGGTACTGAGATCCCTTATCTGGAATCGTCTTCCAGTGGTGCCTCTA
CGGTCGCGTTTAAAAAAGCGGTATTGAGCTTGAAGGTGACACCACAAATCACCCCAGATAACCGCTTGGTGCTGGACTTG
AGCGTGACCCAAGACCGACGCGGTGAAACGGTGAAAACCGGCACTGGCGAAGCGGTTTCTATTGATACTCAGCGTATTGG
CACTCAAGTGTTGGTGAATAACGGAGAAACTGTGGTGTTGGGTGGCATTTTCCAACATAGCATCAATAATTCGGTAGATA
AGGTGCCACTGCTGGGGGATTTGCCTGTTTTGGGCGCGTTATTCAGACGAACCTATGAGCAAATGGGCAAAAGTGAACTG
CTGATTTTTGTCACGCCGAAAGTGGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(411-569)

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K9UP29

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

100

100

1
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

100

100

1
  pilQ Vibrio campbellii strain DS40M4

78.545

96.322

0.757
  pilQ Legionella pneumophila strain ERS1305867

42.778

94.571

0.405
  pilQ Legionella pneumophila strain Lp02

42.778

94.571

0.405
  comQ Acinetobacter baylyi ADP1

39.336

100

0.394
  pilQ Acinetobacter baumannii strain A118

37.819

100

0.389
  pilQ Acinetobacter baumannii D1279779

37.819

100

0.389
  pilQ Pseudomonas aeruginosa PAK

39.344

96.147

0.378

Multiple sequence alignment