Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ACLRDL_RS00430 Genome accession   NZ_CP178387
Coordinates   72334..73350 (-) Length   338 a.a.
NCBI ID   WP_000520444.1    Uniprot ID   A0A0F0B833
Organism   Vibrio cholerae isolate CTMA_1842     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 67334..78350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLRDL_RS00405 (ACLRDL_00405) aroK 68217..68741 (-) 525 WP_000818616.1 shikimate kinase AroK -
  ACLRDL_RS00410 (ACLRDL_00410) pilQ 68928..70643 (-) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  ACLRDL_RS00415 (ACLRDL_00415) pilP 70669..71187 (-) 519 WP_000792033.1 pilus assembly protein PilP Machinery gene
  ACLRDL_RS00420 (ACLRDL_00420) pilO 71177..71767 (-) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACLRDL_RS00425 (ACLRDL_00425) pilN 71760..72344 (-) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  ACLRDL_RS00430 (ACLRDL_00430) pilM 72334..73350 (-) 1017 WP_000520444.1 type IV pilus assembly protein PilM Machinery gene
  ACLRDL_RS00435 (ACLRDL_00435) - 73493..75970 (+) 2478 WP_000668736.1 penicillin-binding protein 1A -
  ACLRDL_RS00440 (ACLRDL_00440) oxyR 76046..76939 (-) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  ACLRDL_RS00445 (ACLRDL_00445) - 77079..77810 (+) 732 WP_000495226.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37707.44 Da        Isoelectric Point: 7.3328

>NTDB_id=1089369 ACLRDL_RS00430 WP_000520444.1 72334..73350(-) (pilM) [Vibrio cholerae isolate CTMA_1842]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=1089369 ACLRDL_RS00430 WP_000520444.1 72334..73350(-) (pilM) [Vibrio cholerae isolate CTMA_1842]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTCTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCATTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F0B833

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

100

100

1
  pilM Vibrio campbellii strain DS40M4

48.521

100

0.485

Multiple sequence alignment