Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   ACLH2T_RS06600 Genome accession   NZ_CP177348
Coordinates   1320710..1321135 (-) Length   141 a.a.
NCBI ID   WP_347920878.1    Uniprot ID   -
Organism   Pediococcus acidilactici strain GR-5     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1290086..1330445 1320710..1321135 within 0


Gene organization within MGE regions


Location: 1290086..1330445
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLH2T_RS06390 (ACLH2T_06390) - 1290086..1290328 (-) 243 WP_152688961.1 hypothetical protein -
  ACLH2T_RS06395 (ACLH2T_06395) - 1290447..1290815 (-) 369 WP_347920918.1 phage holin -
  ACLH2T_RS06400 (ACLH2T_06400) - 1290826..1291089 (-) 264 WP_347921501.1 hemolysin XhlA family protein -
  ACLH2T_RS06405 (ACLH2T_06405) - 1291089..1292252 (-) 1164 WP_347920917.1 GH25 family lysozyme -
  ACLH2T_RS06410 (ACLH2T_06410) - 1292264..1292623 (-) 360 WP_347920915.1 hypothetical protein -
  ACLH2T_RS06415 (ACLH2T_06415) - 1292607..1292768 (-) 162 WP_347920913.1 hypothetical protein -
  ACLH2T_RS06420 (ACLH2T_06420) - 1292772..1293011 (-) 240 WP_347920912.1 hypothetical protein -
  ACLH2T_RS06425 (ACLH2T_06425) - 1293004..1295382 (-) 2379 WP_347920910.1 phage tail protein -
  ACLH2T_RS06430 (ACLH2T_06430) - 1295369..1297744 (-) 2376 WP_347920908.1 phage tail spike protein -
  ACLH2T_RS06435 (ACLH2T_06435) - 1297809..1299578 (-) 1770 WP_347920906.1 phage distal tail protein -
  ACLH2T_RS06440 (ACLH2T_06440) - 1299642..1304321 (-) 4680 WP_347920904.1 peptidoglycan DD-metalloendopeptidase family protein -
  ACLH2T_RS06445 (ACLH2T_06445) - 1304352..1304537 (-) 186 WP_159206364.1 hypothetical protein -
  ACLH2T_RS06450 (ACLH2T_06450) - 1304582..1304956 (-) 375 WP_347920902.1 phage tail assembly chaperone -
  ACLH2T_RS06455 (ACLH2T_06455) - 1305032..1305706 (-) 675 WP_347920901.1 phage tail protein -
  ACLH2T_RS06460 (ACLH2T_06460) - 1305722..1306102 (-) 381 WP_347920899.1 DUF806 family protein -
  ACLH2T_RS06465 (ACLH2T_06465) - 1306102..1306509 (-) 408 WP_347920897.1 HK97-gp10 family putative phage morphogenesis protein -
  ACLH2T_RS06470 (ACLH2T_06470) - 1306512..1306859 (-) 348 WP_152688957.1 phage head closure protein -
  ACLH2T_RS06475 (ACLH2T_06475) - 1306849..1307181 (-) 333 WP_152688956.1 head-tail connector protein -
  ACLH2T_RS06480 (ACLH2T_06480) - 1307254..1308486 (-) 1233 WP_347920896.1 phage major capsid protein -
  ACLH2T_RS06485 (ACLH2T_06485) - 1308486..1309238 (-) 753 WP_347921499.1 head maturation protease, ClpP-related -
  ACLH2T_RS06490 (ACLH2T_06490) - 1309216..1310409 (-) 1194 WP_347920894.1 phage portal protein -
  ACLH2T_RS06495 (ACLH2T_06495) - 1310412..1310606 (-) 195 WP_278892882.1 DUF1056 family protein -
  ACLH2T_RS06500 (ACLH2T_06500) - 1310596..1312494 (-) 1899 WP_347920892.1 terminase large subunit -
  ACLH2T_RS06505 (ACLH2T_06505) - 1312497..1312955 (-) 459 WP_270515254.1 phage terminase small subunit P27 family -
  ACLH2T_RS06510 (ACLH2T_06510) - 1313087..1313449 (-) 363 WP_347921497.1 HNH endonuclease -
  ACLH2T_RS06515 (ACLH2T_06515) - 1313456..1313575 (-) 120 Protein_1261 NUMOD4 domain-containing protein -
  ACLH2T_RS06520 (ACLH2T_06520) - 1313602..1314114 (-) 513 WP_159218494.1 HNH endonuclease -
  ACLH2T_RS06525 (ACLH2T_06525) - 1314083..1314235 (-) 153 WP_163925238.1 hypothetical protein -
  ACLH2T_RS06530 (ACLH2T_06530) - 1314320..1314640 (-) 321 WP_159218495.1 bacteriocin immunity protein -
  ACLH2T_RS06535 (ACLH2T_06535) - 1314928..1315344 (-) 417 WP_159218496.1 ArpU family phage packaging/lysis transcriptional regulator -
  ACLH2T_RS06540 (ACLH2T_06540) - 1315469..1315645 (-) 177 WP_159218497.1 hypothetical protein -
  ACLH2T_RS06545 (ACLH2T_06545) - 1315654..1315932 (-) 279 WP_347920888.1 hypothetical protein -
  ACLH2T_RS06550 (ACLH2T_06550) - 1315925..1316374 (-) 450 WP_159218499.1 DUF1642 domain-containing protein -
  ACLH2T_RS06555 (ACLH2T_06555) - 1316374..1316901 (-) 528 WP_159218500.1 DUF1642 domain-containing protein -
  ACLH2T_RS06560 (ACLH2T_06560) - 1316903..1317043 (-) 141 WP_412052690.1 hypothetical protein -
  ACLH2T_RS06565 (ACLH2T_06565) - 1317071..1317442 (-) 372 WP_159218501.1 N-acetylmuramoyl-L-alanine amidase -
  ACLH2T_RS06570 (ACLH2T_06570) - 1317429..1317578 (-) 150 WP_200834821.1 hypothetical protein -
  ACLH2T_RS06575 (ACLH2T_06575) - 1317575..1317643 (-) 69 WP_235019140.1 hypothetical protein -
  ACLH2T_RS06580 (ACLH2T_06580) - 1317710..1318342 (-) 633 WP_347920885.1 RNA polymerase subunit sigma-70 -
  ACLH2T_RS06585 (ACLH2T_06585) - 1318460..1319242 (-) 783 WP_412052691.1 ATP-binding protein -
  ACLH2T_RS06590 (ACLH2T_06590) - 1319256..1320020 (-) 765 WP_347920881.1 conserved phage C-terminal domain-containing protein -
  ACLH2T_RS06595 (ACLH2T_06595) - 1319998..1320699 (-) 702 WP_274583868.1 putative HNHc nuclease -
  ACLH2T_RS06600 (ACLH2T_06600) ssb 1320710..1321135 (-) 426 WP_347920878.1 single-stranded DNA-binding protein Machinery gene
  ACLH2T_RS06605 (ACLH2T_06605) - 1321128..1321334 (-) 207 WP_024863113.1 hypothetical protein -
  ACLH2T_RS06610 (ACLH2T_06610) - 1321327..1322052 (-) 726 WP_347920876.1 ERF family protein -
  ACLH2T_RS06615 (ACLH2T_06615) - 1322053..1322508 (-) 456 WP_347920874.1 chromosome segregation protein SMC -
  ACLH2T_RS06620 (ACLH2T_06620) - 1322734..1323351 (+) 618 WP_159209346.1 hypothetical protein -
  ACLH2T_RS06625 (ACLH2T_06625) - 1323348..1323521 (-) 174 WP_336118393.1 hypothetical protein -
  ACLH2T_RS06630 (ACLH2T_06630) - 1323622..1323951 (-) 330 WP_347920872.1 DUF771 domain-containing protein -
  ACLH2T_RS06635 (ACLH2T_06635) - 1323965..1324687 (-) 723 WP_347920870.1 Rha family transcriptional regulator -
  ACLH2T_RS06640 (ACLH2T_06640) - 1324747..1325055 (+) 309 WP_160216151.1 hypothetical protein -
  ACLH2T_RS06645 (ACLH2T_06645) - 1325045..1325251 (-) 207 WP_094104442.1 hypothetical protein -
  ACLH2T_RS06650 (ACLH2T_06650) - 1325313..1325516 (+) 204 WP_162013320.1 hypothetical protein -
  ACLH2T_RS06655 (ACLH2T_06655) - 1325703..1325891 (-) 189 WP_159218508.1 hypothetical protein -
  ACLH2T_RS06660 (ACLH2T_06660) - 1326157..1326513 (+) 357 WP_164905797.1 helix-turn-helix domain-containing protein -
  ACLH2T_RS06665 (ACLH2T_06665) - 1326525..1326932 (+) 408 WP_214085115.1 ImmA/IrrE family metallo-endopeptidase -
  ACLH2T_RS06670 (ACLH2T_06670) - 1327002..1327535 (+) 534 WP_115154865.1 PH domain-containing protein -
  ACLH2T_RS06675 (ACLH2T_06675) - 1327687..1327884 (+) 198 WP_053905909.1 hypothetical protein -
  ACLH2T_RS06680 (ACLH2T_06680) - 1328214..1329191 (+) 978 WP_347920865.1 Abi family protein -
  ACLH2T_RS06685 (ACLH2T_06685) - 1329330..1330445 (+) 1116 WP_347920863.1 tyrosine-type recombinase/integrase -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 15626.39 Da        Isoelectric Point: 7.9972

>NTDB_id=1086731 ACLH2T_RS06600 WP_347920878.1 1320710..1321135(-) (ssb) [Pediococcus acidilactici strain GR-5]
MINRTVLIGRLTKDVELRHTAKGDAVASFTVAVNRQFTNSQGEREVDFINCVMWRKAAENFAKYTQKGSLVGIEGRIQTR
SYENQQGQRVYVTEVVAENFSLLDSKPKGNQQNNARQASTPGDPFANGGQSIDIGDDSLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=1086731 ACLH2T_RS06600 WP_347920878.1 1320710..1321135(-) (ssb) [Pediococcus acidilactici strain GR-5]
ATGATTAATCGAACAGTACTAATAGGACGCCTAACTAAAGATGTTGAGCTTCGCCACACGGCTAAAGGCGATGCGGTAGC
TAGTTTTACTGTGGCAGTTAACCGACAGTTTACCAACTCACAGGGTGAACGCGAAGTGGATTTCATCAACTGTGTAATGT
GGCGTAAGGCAGCAGAAAACTTTGCCAAGTACACACAAAAAGGTTCGTTGGTAGGCATTGAGGGTCGAATTCAAACTCGT
TCCTACGAGAATCAACAAGGACAACGAGTGTACGTTACCGAAGTTGTTGCGGAAAACTTCTCGTTGCTGGATTCGAAACC
GAAAGGCAACCAGCAAAATAACGCACGGCAAGCATCAACGCCGGGAGATCCATTCGCTAATGGCGGACAATCAATTGATA
TTGGTGACGATAGTTTGCCTTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

57.059

100

0.688

  ssbA Bacillus subtilis subsp. subtilis str. 168

53.488

100

0.652

  ssbB Bacillus subtilis subsp. subtilis str. 168

60.185

76.596

0.461

  ssbB/cilA Streptococcus mitis NCTC 12261

40.845

100

0.411

  ssbB/cilA Streptococcus pneumoniae TIGR4

40.845

100

0.411

  ssbB/cilA Streptococcus pneumoniae Rx1

40.141

100

0.404

  ssbB/cilA Streptococcus pneumoniae D39

40.141

100

0.404

  ssbB/cilA Streptococcus pneumoniae R6

40.141

100

0.404

  ssbB Streptococcus sobrinus strain NIDR 6715-7

40.141

100

0.404

  ssbB/cilA Streptococcus mitis SK321

40.141

100

0.404

  ssbA Streptococcus mutans UA159

36.62

100

0.369


Multiple sequence alignment