Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   ACLH2T_RS03560 Genome accession   NZ_CP177348
Coordinates   731518..731943 (+) Length   141 a.a.
NCBI ID   WP_347921137.1    Uniprot ID   -
Organism   Pediococcus acidilactici strain GR-5     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 722201..765153 731518..731943 within 0


Gene organization within MGE regions


Location: 722201..765153
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLH2T_RS03470 (ACLH2T_03470) - 722201..722437 (+) 237 WP_002829787.1 hypothetical protein -
  ACLH2T_RS03475 (ACLH2T_03475) - 722721..722975 (+) 255 WP_002829785.1 helix-turn-helix domain-containing protein -
  ACLH2T_RS03485 (ACLH2T_03485) - 723381..724517 (-) 1137 WP_347921146.1 site-specific integrase -
  ACLH2T_RS03490 (ACLH2T_03490) - 724692..725243 (+) 552 WP_098040700.1 hypothetical protein -
  ACLH2T_RS03495 (ACLH2T_03495) - 725233..725364 (-) 132 WP_255031768.1 hypothetical protein -
  ACLH2T_RS03500 (ACLH2T_03500) - 725470..726360 (-) 891 WP_347921143.1 DUF5067 domain-containing protein -
  ACLH2T_RS03505 (ACLH2T_03505) - 726429..726827 (-) 399 WP_098040702.1 hypothetical protein -
  ACLH2T_RS03510 (ACLH2T_03510) - 726862..727292 (-) 431 Protein_688 PH domain-containing protein -
  ACLH2T_RS03515 (ACLH2T_03515) - 727349..727795 (-) 447 WP_235015672.1 ImmA/IrrE family metallo-endopeptidase -
  ACLH2T_RS03520 (ACLH2T_03520) - 727804..728145 (-) 342 WP_098040704.1 helix-turn-helix domain-containing protein -
  ACLH2T_RS03525 (ACLH2T_03525) - 728403..728612 (+) 210 WP_065124225.1 helix-turn-helix domain-containing protein -
  ACLH2T_RS03530 (ACLH2T_03530) - 728626..729360 (+) 735 WP_347921141.1 Rha family transcriptional regulator -
  ACLH2T_RS03535 (ACLH2T_03535) - 729363..729548 (+) 186 WP_347921139.1 hypothetical protein -
  ACLH2T_RS03540 (ACLH2T_03540) - 729566..729904 (+) 339 WP_098040706.1 DUF771 domain-containing protein -
  ACLH2T_RS03545 (ACLH2T_03545) - 730142..730600 (+) 459 WP_235015673.1 chromosome segregation protein SMC -
  ACLH2T_RS03550 (ACLH2T_03550) - 730601..731326 (+) 726 WP_138492715.1 ERF family protein -
  ACLH2T_RS03555 (ACLH2T_03555) - 731319..731525 (+) 207 WP_024863113.1 hypothetical protein -
  ACLH2T_RS03560 (ACLH2T_03560) ssb 731518..731943 (+) 426 WP_347921137.1 single-stranded DNA-binding protein Machinery gene
  ACLH2T_RS03565 (ACLH2T_03565) - 731954..732631 (+) 678 WP_347921136.1 putative HNHc nuclease -
  ACLH2T_RS03570 (ACLH2T_03570) - 732621..732845 (+) 225 WP_347921135.1 hypothetical protein -
  ACLH2T_RS03575 (ACLH2T_03575) - 732875..733618 (+) 744 WP_347921133.1 hypothetical protein -
  ACLH2T_RS03580 (ACLH2T_03580) - 733599..734414 (+) 816 WP_347921131.1 ATP-binding protein -
  ACLH2T_RS03585 (ACLH2T_03585) - 734532..735164 (+) 633 WP_347921129.1 RNA polymerase subunit sigma-70 -
  ACLH2T_RS03590 (ACLH2T_03590) - 735145..735546 (+) 402 WP_204229941.1 RusA family crossover junction endodeoxyribonuclease -
  ACLH2T_RS03595 (ACLH2T_03595) - 735543..735692 (+) 150 WP_317059631.1 hypothetical protein -
  ACLH2T_RS03600 (ACLH2T_03600) - 735679..736050 (+) 372 WP_347921126.1 N-acetylmuramoyl-L-alanine amidase -
  ACLH2T_RS03605 (ACLH2T_03605) - 736092..736232 (+) 141 WP_412052709.1 hypothetical protein -
  ACLH2T_RS03610 (ACLH2T_03610) - 736216..736509 (+) 294 WP_347921122.1 hypothetical protein -
  ACLH2T_RS03615 (ACLH2T_03615) - 736509..736766 (+) 258 WP_347921121.1 hypothetical protein -
  ACLH2T_RS03620 (ACLH2T_03620) - 736767..737060 (+) 294 WP_347921119.1 hypothetical protein -
  ACLH2T_RS03625 (ACLH2T_03625) - 737064..737264 (+) 201 WP_347921117.1 hypothetical protein -
  ACLH2T_RS03630 (ACLH2T_03630) - 737261..737425 (+) 165 WP_347921115.1 hypothetical protein -
  ACLH2T_RS03635 (ACLH2T_03635) - 737607..738023 (+) 417 WP_347921114.1 ArpU family phage packaging/lysis transcriptional regulator -
  ACLH2T_RS03640 (ACLH2T_03640) - 738485..738718 (+) 234 WP_347921112.1 hypothetical protein -
  ACLH2T_RS03645 (ACLH2T_03645) - 738910..739401 (+) 492 WP_347921110.1 terminase small subunit -
  ACLH2T_RS03650 (ACLH2T_03650) - 739394..740740 (+) 1347 WP_347921108.1 PBSX family phage terminase large subunit -
  ACLH2T_RS03655 (ACLH2T_03655) - 740861..742141 (+) 1281 WP_347921478.1 phage portal protein -
  ACLH2T_RS03660 (ACLH2T_03660) - 742125..743090 (+) 966 WP_347921106.1 minor capsid protein -
  ACLH2T_RS03665 (ACLH2T_03665) - 743102..743311 (+) 210 WP_195459779.1 hypothetical protein -
  ACLH2T_RS03670 (ACLH2T_03670) - 743426..744142 (+) 717 WP_058121207.1 DUF4355 domain-containing protein -
  ACLH2T_RS03675 (ACLH2T_03675) - 744142..744960 (+) 819 WP_347921104.1 N4-gp56 family major capsid protein -
  ACLH2T_RS03680 (ACLH2T_03680) - 744978..745244 (+) 267 WP_347921475.1 Ig-like domain-containing protein -
  ACLH2T_RS03685 (ACLH2T_03685) - 745255..745620 (+) 366 WP_176582122.1 phage head-tail connector protein -
  ACLH2T_RS03690 (ACLH2T_03690) - 745625..745933 (+) 309 WP_241531648.1 hypothetical protein -
  ACLH2T_RS03695 (ACLH2T_03695) - 745926..746267 (+) 342 WP_347921101.1 HK97-gp10 family putative phage morphogenesis protein -
  ACLH2T_RS03700 (ACLH2T_03700) - 746292..746675 (+) 384 WP_070366319.1 hypothetical protein -
  ACLH2T_RS03705 (ACLH2T_03705) - 746687..747343 (+) 657 WP_070366318.1 phage major tail protein, TP901-1 family -
  ACLH2T_RS03710 (ACLH2T_03710) - 747416..747757 (+) 342 WP_176582117.1 tail assembly chaperone -
  ACLH2T_RS03715 (ACLH2T_03715) - 747904..748182 (+) 279 WP_347921099.1 hypothetical protein -
  ACLH2T_RS03720 (ACLH2T_03720) - 748166..751186 (+) 3021 WP_347921097.1 phage tail tape measure protein -
  ACLH2T_RS03725 (ACLH2T_03725) - 751186..751950 (+) 765 WP_347921095.1 phage tail protein -
  ACLH2T_RS03730 (ACLH2T_03730) - 751950..754910 (+) 2961 WP_347921093.1 peptidoglycan amidohydrolase family protein -
  ACLH2T_RS03735 (ACLH2T_03735) - 754894..755340 (+) 447 WP_347921091.1 hypothetical protein -
  ACLH2T_RS03740 (ACLH2T_03740) - 755344..757188 (+) 1845 WP_347921089.1 BppU family phage baseplate upper protein -
  ACLH2T_RS03745 (ACLH2T_03745) - 757207..757968 (+) 762 WP_176582256.1 hypothetical protein -
  ACLH2T_RS03750 (ACLH2T_03750) - 757970..758284 (+) 315 WP_347921087.1 DUF2977 domain-containing protein -
  ACLH2T_RS03755 (ACLH2T_03755) - 758284..758430 (+) 147 WP_176582254.1 XkdX family protein -
  ACLH2T_RS03760 (ACLH2T_03760) - 758537..758764 (+) 228 WP_412052710.1 hypothetical protein -
  ACLH2T_RS03765 (ACLH2T_03765) - 758764..759006 (+) 243 WP_347921083.1 phage holin -
  ACLH2T_RS03770 (ACLH2T_03770) - 758990..760111 (+) 1122 WP_347921081.1 peptidoglycan recognition family protein -
  ACLH2T_RS03775 (ACLH2T_03775) - 761236..762213 (+) 978 WP_159208851.1 GMP reductase -
  ACLH2T_RS03780 (ACLH2T_03780) - 762542..764749 (-) 2208 WP_002829782.1 ATP-dependent Clp protease ATP-binding subunit -
  ACLH2T_RS03785 (ACLH2T_03785) - 764965..765153 (+) 189 WP_008842200.1 hypothetical protein -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 15582.30 Da        Isoelectric Point: 8.0068

>NTDB_id=1086726 ACLH2T_RS03560 WP_347921137.1 731518..731943(+) (ssb) [Pediococcus acidilactici strain GR-5]
MINRTVLIGRLTKDVELRHTAKGDAVASFTVAVNRQFTNSQGEREADFINCVMWRKAAENFANFTRKGSLVGIEGRIQTR
SYENQQGQRVYVTEVVADNFSLLDSKPKGNQQNNARQASTPGDPFANGGQSIDIGDDSLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=1086726 ACLH2T_RS03560 WP_347921137.1 731518..731943(+) (ssb) [Pediococcus acidilactici strain GR-5]
ATGATTAATCGAACAGTACTAATAGGACGTTTAACTAAAGATGTTGAGCTTCGCCACACAGCTAAAGGTGATGCGGTAGC
TAGTTTTACCGTGGCAGTTAACCGACAGTTTACCAACTCACAGGGTGAACGTGAAGCGGATTTTATCAACTGCGTAATGT
GGCGGAAAGCGGCGGAAAACTTTGCCAACTTCACGCGTAAAGGTTCTCTAGTAGGCATTGAAGGGCGGATTCAAACCCGT
TCGTACGAAAACCAACAAGGACAACGAGTTTATGTAACTGAGGTTGTAGCTGATAACTTCTCGTTGCTAGATTCGAAACC
AAAAGGCAACCAGCAAAATAACGCACGGCAAGCATCAACGCCGGGAGATCCGTTCGCTAATGGCGGACAATCAATTGATA
TTGGTGACGATAGTTTGCCTTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

59.412

100

0.716

  ssbA Bacillus subtilis subsp. subtilis str. 168

54.651

100

0.667

  ssbB Bacillus subtilis subsp. subtilis str. 168

60.185

76.596

0.461

  ssbB/cilA Streptococcus mitis NCTC 12261

40.845

100

0.411

  ssbB/cilA Streptococcus pneumoniae TIGR4

40.845

100

0.411

  ssbB/cilA Streptococcus pneumoniae Rx1

40.141

100

0.404

  ssbB/cilA Streptococcus pneumoniae D39

40.141

100

0.404

  ssbB/cilA Streptococcus pneumoniae R6

40.141

100

0.404

  ssbB/cilA Streptococcus mitis SK321

40.141

100

0.404

  ssbB Streptococcus sobrinus strain NIDR 6715-7

40.141

100

0.404

  ssbA Streptococcus mutans UA159

36.62

100

0.369


Multiple sequence alignment