Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLD71_RS09290 Genome accession   NZ_CP177241
Coordinates   1958734..1959981 (-) Length   415 a.a.
NCBI ID   WP_411994345.1    Uniprot ID   -
Organism   Agarivorans sp. DSG3-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1953734..1964981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLD71_RS09265 (ACLD71_09265) mutT 1955848..1956255 (+) 408 WP_411994335.1 8-oxo-dGTP diphosphatase MutT -
  ACLD71_RS09270 (ACLD71_09270) yacG 1956252..1956452 (-) 201 WP_411994337.1 DNA gyrase inhibitor YacG -
  ACLD71_RS09275 (ACLD71_09275) zapD 1956452..1957192 (-) 741 WP_411994339.1 cell division protein ZapD -
  ACLD71_RS09280 (ACLD71_09280) coaE 1957251..1957853 (-) 603 WP_411994341.1 dephospho-CoA kinase -
  ACLD71_RS09285 (ACLD71_09285) pilD 1957853..1958731 (-) 879 WP_411994343.1 prepilin peptidase Machinery gene
  ACLD71_RS09290 (ACLD71_09290) pilC 1958734..1959981 (-) 1248 WP_411994345.1 type II secretion system F family protein Machinery gene
  ACLD71_RS09295 (ACLD71_09295) pilB 1960068..1961774 (-) 1707 WP_411994347.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLD71_RS09300 (ACLD71_09300) - 1961781..1962239 (-) 459 WP_411994349.1 pilin -
  ACLD71_RS09305 (ACLD71_09305) nadC 1962494..1963333 (-) 840 WP_411994351.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACLD71_RS09310 (ACLD71_09310) ampD 1963450..1964031 (+) 582 WP_411994353.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACLD71_RS09315 (ACLD71_09315) ampE 1964106..1964960 (+) 855 WP_411994355.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 45467.36 Da        Isoelectric Point: 9.5232

>NTDB_id=1086370 ACLD71_RS09290 WP_411994345.1 1958734..1959981(-) (pilC) [Agarivorans sp. DSG3-1]
MATATSTKRKVAVKKINAYNWSGVNRKGDKVSGEYQAETISQVKAELRRQGITVTKIKKKTESFLSKLGNKINAMDIAVM
SRQIATMLQAGVPLVQSFNIISKSVEKPAMRELISEIAAEVESGTALSETLRKHPLYFDALYCDLIEAGEQSGALETIYD
RVAIYKEKAEALKSKIKKAMFYPVSVIIVAIIVTTILLLFVIPQFEEIFAGFGAELPAFTQFVIAISRFVQDYWPVIFGS
LFALAYGYVQSFRRSQAVKDATDRFVLKIPVINPILHKAAMARFARTLSTTFAAGIPLIDALRSASGASGNVVYRNAIDA
IRIEVTGGMQMNIAMRTVNIFPDMVTQMVMIGEESGSLDSMLEKVANIYEQQVDDAVDGLTSLIEPLIMAVLGVLVGGMV
IAMYLPIFKLGSVVS

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=1086370 ACLD71_RS09290 WP_411994345.1 1958734..1959981(-) (pilC) [Agarivorans sp. DSG3-1]
ATGGCTACCGCAACCAGCACAAAACGAAAAGTCGCCGTCAAAAAAATCAACGCTTATAATTGGAGTGGGGTAAACCGTAA
AGGCGATAAGGTTAGCGGAGAATACCAAGCTGAAACCATTAGTCAGGTTAAGGCAGAACTACGTAGACAAGGCATTACCG
TAACAAAAATTAAAAAGAAAACAGAAAGTTTTCTTTCTAAGCTCGGTAATAAAATTAATGCCATGGACATTGCCGTTATG
TCGCGGCAAATAGCAACCATGCTGCAAGCAGGTGTACCTTTAGTACAAAGCTTTAATATTATTTCAAAAAGTGTCGAAAA
GCCGGCGATGAGGGAGCTTATTTCCGAAATAGCGGCGGAGGTTGAGTCAGGTACCGCGCTATCAGAAACCTTGCGTAAAC
ACCCGCTCTACTTCGATGCCTTATATTGTGACTTAATTGAAGCTGGCGAACAAAGTGGTGCTTTAGAGACGATTTATGAC
CGCGTAGCAATTTATAAAGAAAAAGCAGAAGCGCTTAAATCAAAAATTAAAAAAGCCATGTTTTACCCCGTCTCAGTTAT
TATCGTTGCAATTATCGTTACTACTATCCTTTTACTGTTTGTAATTCCACAATTCGAAGAAATTTTTGCAGGTTTTGGAG
CAGAGCTTCCAGCATTTACCCAGTTTGTTATTGCTATATCTCGTTTTGTACAGGACTATTGGCCAGTCATTTTTGGTTCC
TTGTTTGCTTTAGCTTATGGCTATGTACAGTCCTTTAGGCGATCCCAAGCAGTTAAAGACGCCACTGACCGGTTCGTTCT
TAAAATTCCGGTCATTAACCCTATTTTACATAAAGCAGCTATGGCCCGTTTTGCTCGTACCTTATCAACGACCTTTGCTG
CAGGTATCCCATTAATTGATGCGCTTCGCTCGGCTTCAGGGGCTTCAGGCAACGTTGTATACCGCAATGCCATTGACGCA
ATTAGAATTGAGGTTACAGGTGGTATGCAAATGAATATCGCCATGCGAACCGTTAATATATTTCCAGACATGGTGACGCA
AATGGTGATGATTGGCGAAGAGTCTGGCTCCTTAGATAGCATGCTAGAAAAAGTAGCCAATATTTACGAGCAACAAGTAG
ATGACGCTGTTGATGGCCTCACAAGCCTGATCGAACCACTTATTATGGCTGTGCTTGGCGTATTGGTTGGTGGTATGGTA
ATCGCCATGTATCTACCAATCTTTAAATTAGGCTCAGTTGTCAGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(284-406)

Type II secretion system (T2SS), protein F (T2SSF)

(81-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.75

96.386

0.537
  pilC Acinetobacter baumannii D1279779

53.676

98.313

0.528
  pilC Acinetobacter baylyi ADP1

54.156

95.663

0.518
  pilC Legionella pneumophila strain ERS1305867

52.525

95.422

0.501
  pilC Vibrio campbellii strain DS40M4

45.32

97.831

0.443
  pilC Vibrio cholerae strain A1552

45.363

96.145

0.436
  pilG Neisseria gonorrhoeae MS11

43.21

97.59

0.422
  pilG Neisseria meningitidis 44/76-A

43.21

97.59

0.422
  pilC Thermus thermophilus HB27

37.469

97.108

0.364

Multiple sequence alignment