Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLD71_RS09295 Genome accession   NZ_CP177241
Coordinates   1960068..1961774 (-) Length   568 a.a.
NCBI ID   WP_411994347.1    Uniprot ID   -
Organism   Agarivorans sp. DSG3-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1955068..1966774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLD71_RS09265 (ACLD71_09265) mutT 1955848..1956255 (+) 408 WP_411994335.1 8-oxo-dGTP diphosphatase MutT -
  ACLD71_RS09270 (ACLD71_09270) yacG 1956252..1956452 (-) 201 WP_411994337.1 DNA gyrase inhibitor YacG -
  ACLD71_RS09275 (ACLD71_09275) zapD 1956452..1957192 (-) 741 WP_411994339.1 cell division protein ZapD -
  ACLD71_RS09280 (ACLD71_09280) coaE 1957251..1957853 (-) 603 WP_411994341.1 dephospho-CoA kinase -
  ACLD71_RS09285 (ACLD71_09285) pilD 1957853..1958731 (-) 879 WP_411994343.1 prepilin peptidase Machinery gene
  ACLD71_RS09290 (ACLD71_09290) pilC 1958734..1959981 (-) 1248 WP_411994345.1 type II secretion system F family protein Machinery gene
  ACLD71_RS09295 (ACLD71_09295) pilB 1960068..1961774 (-) 1707 WP_411994347.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLD71_RS09300 (ACLD71_09300) - 1961781..1962239 (-) 459 WP_411994349.1 pilin -
  ACLD71_RS09305 (ACLD71_09305) nadC 1962494..1963333 (-) 840 WP_411994351.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACLD71_RS09310 (ACLD71_09310) ampD 1963450..1964031 (+) 582 WP_411994353.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACLD71_RS09315 (ACLD71_09315) ampE 1964106..1964960 (+) 855 WP_411994355.1 beta-lactamase regulator AmpE -
  ACLD71_RS09320 (ACLD71_09320) pdhR 1965218..1965982 (+) 765 WP_137673701.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62937.57 Da        Isoelectric Point: 4.9486

>NTDB_id=1086371 ACLD71_RS09295 WP_411994347.1 1960068..1961774(-) (pilB) [Agarivorans sp. DSG3-1]
MSVEIKLNGLASSLAVSFPEKETQLVELFKRCIQEQRAFTTVLVENELISSKDLADFCEAQFGIPLLDLDAIELTEIPQD
YLNEKLIFKHHVLPVYSQNQTLYVAMADPSNVVALEDFGFSFGLHVEALLVEETKLLKAIQSLVDDTSNLDLGDISDEDI
SGLEVSDENSRLDEKQDNKEDDAPIVVYINKILMDSIRRGASDLHFEPYEHKYRIRFRIDGILHEIASPPINLANRFSAR
LKVMARMDIAERRLPQDGRIKLKTGRNKAIDMRVNSLPTMWGEKIVIRILDSSAANLNIDILGYDDKQKKLYLDALARPQ
GMILVTGPTGSGKTVSLYTGLNILNTTETNISTAEDPVEINLPGVNQVLINNKAGMTFASALRAFLRQDPDIVMVGEIRD
LETAEISIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPAFNIASSVSLIIAQRLARKLCEHCKKPKMVEAILLPKMGFT
QTQIDSGIKLFEPVGCDKCTGGYKGRVGIYEVMPMSTEISKLIMLEGNSIQIAEQAAIEGMYSLRMSALEKARLGVTSLS
EVERVTNV

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1086371 ACLD71_RS09295 WP_411994347.1 1960068..1961774(-) (pilB) [Agarivorans sp. DSG3-1]
ATGTCAGTCGAAATTAAACTTAACGGCCTAGCTTCTAGCTTGGCCGTTTCTTTTCCAGAAAAAGAAACTCAACTTGTGGA
GTTGTTCAAACGCTGCATTCAAGAACAGCGCGCCTTTACAACAGTCCTTGTAGAAAACGAACTAATTTCGAGTAAAGATC
TTGCCGATTTCTGTGAAGCGCAGTTTGGCATTCCCTTACTCGATCTCGATGCCATTGAACTAACCGAAATACCGCAAGAC
TACCTTAACGAAAAGCTCATTTTTAAACACCACGTACTGCCTGTTTATAGCCAAAACCAAACTCTTTATGTAGCAATGGC
GGATCCAAGTAACGTTGTTGCTTTGGAAGATTTTGGTTTTAGCTTTGGACTACATGTCGAAGCACTTTTGGTTGAAGAAA
CTAAGCTCCTCAAAGCCATTCAGTCTTTAGTTGACGATACATCTAATCTTGACCTCGGCGATATCTCAGATGAAGATATT
AGTGGCCTAGAGGTGAGCGATGAAAACTCTCGGCTTGATGAAAAGCAAGATAATAAAGAAGATGATGCACCGATTGTTGT
CTACATCAACAAGATATTGATGGATTCAATTCGCCGTGGTGCTTCAGATTTACACTTTGAACCTTACGAGCATAAATACC
GGATCCGTTTTCGCATTGACGGTATCTTGCATGAAATAGCCAGCCCGCCGATTAATTTAGCCAACCGCTTCTCAGCTCGC
TTGAAAGTAATGGCCAGAATGGATATCGCCGAGCGCCGATTGCCGCAAGATGGACGAATTAAGCTGAAAACTGGGCGAAA
CAAAGCCATTGATATGCGGGTTAACTCGCTGCCCACCATGTGGGGAGAAAAAATTGTAATTCGTATATTAGATTCCTCAG
CAGCCAATCTAAATATCGATATCCTCGGTTATGATGACAAGCAAAAGAAACTATACCTAGACGCTTTAGCTCGGCCACAA
GGTATGATCTTGGTAACCGGCCCTACCGGTTCTGGTAAAACAGTATCTTTGTACACTGGCTTGAACATTCTAAACACTAC
CGAAACAAATATTTCTACAGCGGAAGACCCTGTGGAAATCAACTTGCCAGGTGTAAATCAAGTATTGATTAACAATAAAG
CGGGAATGACCTTTGCCAGCGCTTTACGTGCATTTTTACGTCAAGATCCCGATATTGTCATGGTGGGGGAGATTCGAGAT
TTAGAAACTGCTGAAATTTCGATTAAGGCAGCACAAACTGGTCACTTAGTTTTATCTACTTTACATACCAATTCAGCGGC
AGAGACTTTAACTCGGCTGTTAAACATGGGTGTCCCCGCCTTTAACATTGCTTCTTCCGTTAGTTTAATAATTGCCCAGC
GACTAGCCCGTAAATTATGTGAACATTGTAAAAAACCGAAAATGGTTGAAGCCATATTGCTACCCAAAATGGGCTTCACT
CAAACACAAATCGACTCTGGTATTAAGCTATTTGAGCCCGTTGGCTGTGACAAATGCACGGGCGGATACAAGGGACGAGT
AGGTATTTATGAGGTCATGCCTATGAGTACTGAAATTTCCAAGTTGATTATGTTAGAAGGTAATTCAATTCAAATCGCAG
AGCAAGCGGCCATTGAGGGCATGTATTCACTACGTATGTCTGCCTTAGAAAAAGCCCGCTTAGGCGTAACTAGTCTTTCT
GAGGTAGAGCGGGTCACAAATGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.957

94.894

0.54
  pilB Acinetobacter baylyi ADP1

57.386

92.958

0.533
  pilB Legionella pneumophila strain ERS1305867

52.797

100

0.532
  pilB Vibrio cholerae strain A1552

54.34

93.31

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

53.977

92.958

0.502
  pilB Vibrio campbellii strain DS40M4

53.308

93.134

0.496
  pilF Neisseria gonorrhoeae MS11

48.96

93.134

0.456
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.252

94.19

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

39.961

91.197

0.364

Multiple sequence alignment