Detailed information NTG1086333

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACK3BE_RS17235
• Genome accession: NZ_CP177237
• Coordinates: 3818277..3819386 (-)
• Length: 369 a.a.
• NCBI ID: WP_411959738.1
• Uniprot ID: -
• Organism: Pseudomonas mandelii strain VR4' HT30-3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3813277..3824386

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK3BE_RS17230 (ACK3BE_17230)	-	3814291..3818151 (-)	3861	WP_411959737.1	hypothetical protein	-
ACK3BE_RS17235 (ACK3BE_17235)	pilU	3818277..3819386 (-)	1110	WP_411959738.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACK3BE_RS17240 (ACK3BE_17240)	-	3819508..3822045 (+)	2538	WP_077747701.1	PAS domain-containing protein	-
ACK3BE_RS17245 (ACK3BE_17245)	-	3822112..3822387 (+)	276	WP_007898282.1	peptidylprolyl isomerase	-
ACK3BE_RS17250 (ACK3BE_17250)	-	3822509..3823444 (-)	936	WP_083376588.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41083.98 Da
• Isoelectric Point: 6.2376

>NTDB_id=1086333 ACK3BE_RS17235 WP_411959738.1 3818277..3819386(-) (pilU) [Pseudomonas mandelii strain VR4' HT30-3]
MEIDALLQILSDRNGSDLYLSTGAPPSARFDGVLKPLTDQPFKPGAVAAIAESIMDAEQRLEFDRELEMNLAISLAGIGR
FRVNIFKQRNDVSIVARNIKLDIPRFEDLKLPAVLLETVMLKQGLILFVGATDCGKSTSLAALIDYRNRHSTGHIVTIED
PIEYIHRHKQSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNAHQALDRI
INFFPEERRTQLLHDLSNNLKAFVSQRLVRTRDGQRRAAVEVMLGTPTIGDLIRRNQLDELKDIMEKSAEFGMQTFDGAL
YALVMEGAISEEEALKHADSLSNLKLRLKLYGGGPSTVNAPNGDWGLVD

Nucleotide

• Length: 1110 bp

>NTDB_id=1086333 ACK3BE_RS17235 WP_411959738.1 3818277..3819386(-) (pilU) [Pseudomonas mandelii strain VR4' HT30-3]
ATGGAAATCGATGCACTGTTGCAGATACTGTCTGACCGGAATGGATCCGACCTCTATCTTTCCACCGGCGCACCACCCAG
TGCGCGGTTCGACGGAGTGCTCAAGCCGCTGACCGACCAGCCATTCAAGCCGGGAGCAGTCGCGGCCATTGCCGAGTCCA
TCATGGACGCCGAACAGCGCCTGGAGTTCGATCGGGAACTGGAGATGAACCTGGCAATCTCCCTGGCGGGTATCGGGCGC
TTTCGGGTCAACATCTTCAAGCAACGCAACGATGTGTCAATCGTGGCACGCAACATCAAGCTCGACATCCCGCGTTTCGA
AGACCTCAAACTACCGGCGGTGCTGCTCGAAACCGTGATGCTCAAACAAGGATTGATACTGTTCGTCGGCGCCACCGATT
GCGGCAAGTCAACCTCCCTCGCGGCGCTGATCGATTACCGCAACCGCCACAGCACTGGCCATATCGTCACCATCGAGGAC
CCGATTGAGTACATCCATCGGCACAAGCAGTCGATCATCAACCAGCGCGAGGTCGGCGTCGATACCCGCAGTTTTCATGC
TGCCCTGAAAAACACCCTGCGCCAGGCCCCGGACGTGGTACTGATCGGCGAAATCCGCGACCGCGAAACCATGGAGCACG
CGCTGGCGTTTGCCGATACGGGCCATCTGGTGATCTCGACGTTGCACGCGCACAACGCCCATCAGGCGCTGGACCGCATC
ATCAATTTCTTTCCCGAAGAGCGGCGGACGCAGTTGCTGCATGATTTGAGCAACAATCTGAAAGCGTTTGTTTCCCAACG
ATTGGTGCGGACCCGTGACGGTCAGCGCAGGGCGGCAGTGGAAGTGATGTTGGGAACACCCACGATCGGTGACTTGATAA
GGCGCAATCAACTGGACGAACTTAAAGACATCATGGAAAAGTCCGCAGAGTTCGGTATGCAGACGTTCGATGGCGCACTG
TATGCACTGGTGATGGAAGGCGCAATCAGTGAGGAAGAGGCGCTAAAACATGCGGACTCGCTGAGCAATCTGAAGTTGCG
CCTCAAGCTGTATGGCGGTGGGCCTTCGACCGTCAATGCGCCCAATGGGGACTGGGGGTTAGTGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.84	95.122	0.531
pilU	Acinetobacter baylyi ADP1	51.989	95.393	0.496
pilU	Vibrio cholerae strain A1552	50.833	97.561	0.496
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.456	95.122	0.385
pilT	Vibrio cholerae strain A1552	40.456	95.122	0.385
pilT	Pseudomonas aeruginosa PAK	39.826	93.225	0.371
pilT	Legionella pneumophila strain Lp02	41.018	90.515	0.371
pilT	Legionella pneumophila strain ERS1305867	41.018	90.515	0.371
pilT	Pseudomonas stutzeri DSM 10701	39.535	93.225	0.369
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.233	91.87	0.36