Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACKWD1_RS15435 Genome accession   NZ_CP177201
Coordinates   3303302..3304543 (+) Length   413 a.a.
NCBI ID   WP_143238137.1    Uniprot ID   -
Organism   Aeromonas caviae strain QZ63     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3298302..3309543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKWD1_RS15410 (ACKWD1_15410) ampD 3298570..3299142 (-) 573 WP_053288099.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACKWD1_RS15415 (ACKWD1_15415) - 3299271..3299741 (+) 471 WP_201985193.1 TIGR02281 family clan AA aspartic protease -
  ACKWD1_RS15420 (ACKWD1_15420) nadC 3299745..3300608 (+) 864 WP_042864860.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACKWD1_RS15425 (ACKWD1_15425) - 3300919..3301464 (+) 546 WP_113069552.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACKWD1_RS15430 (ACKWD1_15430) pilB 3301468..3303174 (+) 1707 WP_201985192.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ACKWD1_RS15435 (ACKWD1_15435) pilC 3303302..3304543 (+) 1242 WP_143238137.1 type II secretion system F family protein Machinery gene
  ACKWD1_RS15440 (ACKWD1_15440) pilD 3304621..3305496 (+) 876 WP_201985191.1 prepilin peptidase Machinery gene
  ACKWD1_RS15445 (ACKWD1_15445) coaE 3305515..3306129 (+) 615 WP_201985190.1 dephospho-CoA kinase -
  ACKWD1_RS15450 (ACKWD1_15450) zapD 3306167..3306889 (+) 723 WP_010675825.1 cell division protein ZapD -
  ACKWD1_RS15455 (ACKWD1_15455) yacG 3306900..3307094 (+) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  ACKWD1_RS15460 (ACKWD1_15460) mutT 3307153..3307557 (-) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -
  ACKWD1_RS15465 (ACKWD1_15465) - 3307566..3308750 (-) 1185 WP_201985189.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45447.42 Da        Isoelectric Point: 9.9380

>NTDB_id=1086140 ACKWD1_RS15435 WP_143238137.1 3303302..3304543(+) (pilC) [Aeromonas caviae strain QZ63]
MATLAQKRNAPKKVFSYRWHGVNRKGQKVSGELQADSIITVKAELRKQGVNVTRVSKQSQGLFSKGGAKIKPMDIAVISR
QITTMLSAGVPLVQSLQIIARGHEKAAVRELIGQIAADVETGTPLSEALRRHPRHFDDLYCDLVEAGEQSGALETIYDRI
AIYREKNEALKSKIKKAMFYPSMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPAFTRFVIAISRFMQEWWYAIFGGAA
LAVFLYVRAWRKSQKVRDNTDKFILTIPVVGNILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTATMAIR
NEVVAGMQINVAMRTVDLFPDMVTQMVMIGEESGAIDDMLSKVAAIFEQEVDDMVDGLTSLLEPIIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=1086140 ACKWD1_RS15435 WP_143238137.1 3303302..3304543(+) (pilC) [Aeromonas caviae strain QZ63]
ATGGCCACATTAGCTCAAAAACGCAACGCACCGAAAAAAGTCTTCTCCTACCGCTGGCACGGCGTCAACCGCAAGGGACA
GAAGGTCTCCGGCGAGCTGCAAGCCGACAGCATCATCACCGTCAAGGCCGAGCTGCGCAAGCAGGGCGTCAACGTCACCA
GGGTCAGCAAGCAGAGCCAGGGGCTCTTCTCCAAGGGCGGCGCCAAGATCAAGCCGATGGACATCGCCGTCATCTCCCGC
CAGATCACCACCATGCTCTCCGCCGGCGTGCCTCTGGTGCAGAGTCTTCAGATCATTGCTCGCGGCCACGAGAAAGCGGC
GGTGCGCGAACTCATCGGCCAGATCGCCGCCGATGTGGAAACGGGCACCCCGCTCTCGGAGGCGTTGCGCCGCCATCCCC
GCCACTTCGACGACCTCTATTGCGACCTGGTGGAGGCCGGGGAGCAATCCGGTGCCCTGGAGACCATCTACGACCGTATC
GCCATCTACCGCGAGAAGAACGAAGCCCTCAAATCCAAGATCAAGAAGGCCATGTTCTATCCGTCCATGGTCATTCTGGT
GGCCATCGTCGTCACCTCCATCCTGCTGCTCTTCGTCATTCCCCAGTTCGAGGATATCTTCAAGAGCTTCGGTGCCGAGC
TGCCCGCCTTCACCCGGTTCGTCATCGCCATCTCCCGCTTTATGCAGGAGTGGTGGTACGCCATCTTTGGCGGCGCTGCT
CTCGCCGTCTTCCTCTATGTGCGGGCCTGGCGAAAATCCCAGAAGGTCAGGGACAACACGGACAAGTTCATCCTCACCAT
TCCCGTGGTGGGCAACATACTGCACAAGGCTGCCATGGCCCGTTTTGCCCGCACCCTCTCCACCACCTTCTCCGCCGGTA
TCCCACTGGTGGATGCCCTGGTCTCAGCGGCCGGAGCATCGGGCAACTATGTCTATCGCACTGCGACCATGGCCATTCGC
AACGAGGTGGTGGCCGGCATGCAGATCAACGTCGCCATGCGCACCGTGGATCTTTTCCCCGACATGGTGACCCAGATGGT
GATGATCGGTGAGGAATCCGGCGCCATCGACGACATGCTCTCCAAGGTCGCCGCCATTTTCGAACAGGAAGTGGACGACA
TGGTCGACGGCCTCACCAGCCTGCTCGAACCCATCATCATGGTGGTGCTCGGGGTGCTGGTCGGCGGCATGGTCGTGGCC
ATGTACCTCCCCATTTTCAAGCTGGGCTCTGTGATACACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.566

95.884

0.542
  pilC Legionella pneumophila strain ERS1305867

52.099

98.063

0.511
  pilC Acinetobacter baylyi ADP1

50.971

99.758

0.508
  pilC Acinetobacter baumannii D1279779

52.369

97.094

0.508
  pilC Vibrio cholerae strain A1552

47.619

96.61

0.46
  pilC Vibrio campbellii strain DS40M4

45.363

96.61

0.438
  pilG Neisseria meningitidis 44/76-A

41.602

93.705

0.39
  pilG Neisseria gonorrhoeae MS11

41.602

93.705

0.39
  pilC Thermus thermophilus HB27

38.75

96.852

0.375

Multiple sequence alignment