Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACKWD1_RS15430 Genome accession   NZ_CP177201
Coordinates   3301468..3303174 (+) Length   568 a.a.
NCBI ID   WP_201985192.1    Uniprot ID   -
Organism   Aeromonas caviae strain QZ63     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3296468..3308174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKWD1_RS15405 (ACKWD1_15405) pdhR 3297529..3298293 (-) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ACKWD1_RS15410 (ACKWD1_15410) ampD 3298570..3299142 (-) 573 WP_053288099.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACKWD1_RS15415 (ACKWD1_15415) - 3299271..3299741 (+) 471 WP_201985193.1 TIGR02281 family clan AA aspartic protease -
  ACKWD1_RS15420 (ACKWD1_15420) nadC 3299745..3300608 (+) 864 WP_042864860.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACKWD1_RS15425 (ACKWD1_15425) - 3300919..3301464 (+) 546 WP_113069552.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACKWD1_RS15430 (ACKWD1_15430) pilB 3301468..3303174 (+) 1707 WP_201985192.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ACKWD1_RS15435 (ACKWD1_15435) pilC 3303302..3304543 (+) 1242 WP_143238137.1 type II secretion system F family protein Machinery gene
  ACKWD1_RS15440 (ACKWD1_15440) pilD 3304621..3305496 (+) 876 WP_201985191.1 prepilin peptidase Machinery gene
  ACKWD1_RS15445 (ACKWD1_15445) coaE 3305515..3306129 (+) 615 WP_201985190.1 dephospho-CoA kinase -
  ACKWD1_RS15450 (ACKWD1_15450) zapD 3306167..3306889 (+) 723 WP_010675825.1 cell division protein ZapD -
  ACKWD1_RS15455 (ACKWD1_15455) yacG 3306900..3307094 (+) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  ACKWD1_RS15460 (ACKWD1_15460) mutT 3307153..3307557 (-) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62580.97 Da        Isoelectric Point: 5.8214

>NTDB_id=1086139 ACKWD1_RS15430 WP_201985192.1 3301468..3303174(+) (pilB) [Aeromonas caviae strain QZ63]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVLRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=1086139 ACKWD1_RS15430 WP_201985192.1 3301468..3303174(+) (pilB) [Aeromonas caviae strain QZ63]
ATGACCTCTAGCCTCAATAGCGGCCTGGCCACCAGCCTGGCCGCTTCTTCCCTCCTCAGTGAGCCAGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAACGACATCCTCGACAGCAAAGCCCTCG
CCGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTTGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATCTACACCCAGGGGCATACCCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTTGGCTTCAGCTTCGGCCTGCACACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTCTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCCCATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGATAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCGGCTCGC
CTCAAGGTGATGGCGCGGCTCGACATTGCCGAACGGCGCCTGCCCCAGGATGGCCGCATCAAGCTCAAGATCTCGCGCAA
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGCTCCGGTTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCGATCAGCTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTCACCGGTCCCACGGGGTCTGGCAAGACGGTCTCACTCTATACCGGCCTCAACATTCTCAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTCGAGATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAAG
CAGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAAGATCCCGACATCGTCATGGTGGGGGAGATACGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTTACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAGCCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATCTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAGAAGGAAGGGATGCGCACCCTGCGTACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.026

99.824

0.549
  pilB Acinetobacter baylyi ADP1

55.062

99.12

0.546
  pilB Legionella pneumophila strain ERS1305867

54.867

99.472

0.546
  pilB Vibrio cholerae strain A1552

53.59

100

0.539
  pilB Vibrio campbellii strain DS40M4

50.707

99.648

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.266

99.12

0.498
  pilF Neisseria gonorrhoeae MS11

46.384

99.824

0.463
  pilF Thermus thermophilus HB27

40.341

92.958

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

38.156

97.359

0.371

Multiple sequence alignment