Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLIN3_RS05465 Genome accession   NZ_CP176868
Coordinates   1234466..1235674 (-) Length   402 a.a.
NCBI ID   WP_411566255.1    Uniprot ID   -
Organism   Pseudomonas orientalis strain MPB20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1229466..1240674
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLIN3_RS05425 (ACLIN3_05425) - 1229476..1230774 (-) 1299 WP_181282728.1 NAD(P)/FAD-dependent oxidoreductase -
  ACLIN3_RS05430 (ACLIN3_05430) - 1230835..1231008 (-) 174 WP_065887994.1 DUF3094 family protein -
  ACLIN3_RS05435 (ACLIN3_05435) - 1231062..1231691 (-) 630 WP_181282727.1 DUF1780 domain-containing protein -
  ACLIN3_RS05440 (ACLIN3_05440) - 1231821..1232510 (+) 690 WP_191956596.1 energy-coupling factor ABC transporter permease -
  ACLIN3_RS05445 (ACLIN3_05445) - 1232574..1232789 (+) 216 WP_181282725.1 hypothetical protein -
  ACLIN3_RS05450 (ACLIN3_05450) yacG 1232786..1232986 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  ACLIN3_RS05455 (ACLIN3_05455) coaE 1232983..1233606 (-) 624 WP_181282724.1 dephospho-CoA kinase -
  ACLIN3_RS05460 (ACLIN3_05460) pilD 1233603..1234469 (-) 867 WP_411566254.1 prepilin peptidase Machinery gene
  ACLIN3_RS05465 (ACLIN3_05465) pilC 1234466..1235674 (-) 1209 WP_411566255.1 type II secretion system F family protein Machinery gene
  ACLIN3_RS05470 (ACLIN3_05470) - 1235873..1236286 (+) 414 WP_411566718.1 pilin -
  ACLIN3_RS05480 (ACLIN3_05480) - 1236600..1236905 (+) 306 WP_181282722.1 DUF6388 family protein -
  ACLIN3_RS05485 (ACLIN3_05485) nadC 1237025..1237873 (-) 849 WP_243353320.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACLIN3_RS05490 (ACLIN3_05490) - 1238117..1240273 (+) 2157 WP_181282720.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43289.70 Da        Isoelectric Point: 9.7268

>NTDB_id=1083436 ACLIN3_RS05465 WP_411566255.1 1234466..1235674(-) (pilC) [Pseudomonas orientalis strain MPB20]
MKDSSTIYAWQGINRKGHWVCGKIAARDLAQVNAQLREQGICPERVRRQSPGLPSLAAPILPADIALFTRQLATLLKAGI
PLLQAFDIISAGVENRTLRELVKGLKQHVAAGNSLTSTLRQYPRYFDDLYCHLIAAGEQAGALETLLERVAIHLEKSQRL
KTRIKKAMTYPLAVLVVAGLVSAVLLIQVVPQFQNLFAGVDSQLPAFTLAVIALSDFLQQAWWMLALGLGTAFGGLRHAY
RGSAGFRYRLEAGLLKVPLAGKLLTKAAVARYARTLATTFAAGVPLVQALGSVAGAVGNGPFKQAIEHMRHDVSTGMQLN
QSMAANGLFPGMAIQMTAIGEESGTLDHMLEKVANHYEADVETLVDNLTSLMEPLIMVVLGSIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=1083436 ACLIN3_RS05465 WP_411566255.1 1234466..1235674(-) (pilC) [Pseudomonas orientalis strain MPB20]
ATGAAAGACAGCTCGACGATCTACGCCTGGCAGGGCATCAACCGCAAAGGCCACTGGGTGTGCGGAAAAATCGCGGCGCG
TGACCTTGCGCAAGTCAATGCACAACTGCGCGAACAAGGAATCTGCCCCGAGCGTGTGCGCAGGCAATCGCCAGGCCTGC
CAAGCCTGGCTGCGCCGATACTGCCGGCGGACATTGCCCTGTTCACCCGCCAGTTGGCGACGCTGTTGAAGGCGGGTATC
CCGCTGCTGCAAGCCTTCGACATCATCAGCGCGGGCGTTGAAAACCGCACACTGCGCGAGCTGGTCAAGGGTCTGAAGCA
ACACGTCGCCGCCGGTAACAGCCTGACCAGCACGCTGCGCCAATATCCCCGTTACTTCGATGACCTGTACTGCCACTTGA
TTGCCGCCGGTGAACAGGCCGGTGCCCTGGAAACGCTGCTGGAGCGCGTGGCCATTCATCTGGAAAAAAGCCAGCGGCTC
AAAACCAGAATCAAAAAAGCCATGACCTACCCTCTTGCAGTGCTGGTGGTGGCCGGACTGGTCAGCGCAGTGTTGTTGAT
ACAAGTGGTGCCGCAGTTCCAGAACCTGTTCGCCGGAGTCGACAGCCAATTGCCCGCTTTTACCCTCGCGGTCATCGCCC
TTTCCGATTTTCTGCAACAGGCCTGGTGGATGCTGGCGCTGGGCCTGGGCACGGCCTTCGGCGGGCTGCGTCACGCCTAC
CGTGGCTCTGCGGGGTTTCGCTATCGACTGGAGGCGGGTTTGTTGAAAGTCCCCCTGGCAGGCAAACTGTTGACCAAAGC
TGCCGTCGCACGCTACGCCCGCACGCTTGCAACCACCTTTGCGGCAGGTGTCCCGCTGGTGCAGGCGCTGGGCTCAGTGG
CCGGCGCTGTCGGCAATGGCCCTTTCAAACAGGCAATCGAACATATGCGCCACGATGTATCCACAGGCATGCAGTTGAAT
CAATCCATGGCCGCCAACGGCCTGTTTCCAGGCATGGCGATCCAGATGACGGCGATCGGCGAAGAGTCCGGCACCCTGGA
CCACATGCTGGAAAAAGTTGCGAACCATTACGAGGCCGACGTAGAGACCCTCGTCGATAACCTCACCAGCCTGATGGAAC
CGCTGATCATGGTGGTGCTGGGGAGCATCGTCGGCGCGCTGGTGATTGCAATGTACCTGCCGGTATTTCAACTGGGTTCG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.035

99.005

0.565
  pilC Acinetobacter baylyi ADP1

51.515

98.507

0.507
  pilC Acinetobacter baumannii D1279779

49.114

98.259

0.483
  pilC Legionella pneumophila strain ERS1305867

48.354

98.259

0.475
  pilG Neisseria gonorrhoeae MS11

41.207

94.776

0.391
  pilG Neisseria meningitidis 44/76-A

40.682

94.776

0.386
  pilC Vibrio cholerae strain A1552

37.838

100

0.383
  pilC Thermus thermophilus HB27

37.845

99.254

0.376
  pilC Vibrio campbellii strain DS40M4

37.817

98.01

0.371

Multiple sequence alignment