Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACK8N4_RS25410 Genome accession   NZ_CP176811
Coordinates   5418694..5419911 (+) Length   405 a.a.
NCBI ID   WP_114232521.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain DG-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5413694..5424911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK8N4_RS25385 (ACK8N4_25385) - 5414274..5414387 (-) 114 Protein_5021 pilin -
  ACK8N4_RS25390 (ACK8N4_25390) tnpB 5414464..5414823 (+) 360 WP_077473005.1 IS66 family insertion sequence element accessory protein TnpB -
  ACK8N4_RS25395 (ACK8N4_25395) tnpC 5414843..5416348 (+) 1506 WP_121324304.1 IS66 family transposase -
  ACK8N4_RS25400 (ACK8N4_25400) - 5416391..5416759 (-) 369 Protein_5024 pilin -
  ACK8N4_RS25405 (ACK8N4_25405) pilB 5416990..5418690 (+) 1701 WP_114232520.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACK8N4_RS25410 (ACK8N4_25410) pilC 5418694..5419911 (+) 1218 WP_114232521.1 type II secretion system F family protein Machinery gene
  ACK8N4_RS25415 (ACK8N4_25415) pilD 5419912..5420784 (+) 873 WP_114232522.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACK8N4_RS25420 (ACK8N4_25420) coaE 5420781..5421392 (+) 612 WP_033970460.1 dephospho-CoA kinase -
  ACK8N4_RS25425 (ACK8N4_25425) yacG 5421389..5421589 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  ACK8N4_RS25430 (ACK8N4_25430) - 5421626..5421835 (-) 210 WP_003094660.1 hypothetical protein -
  ACK8N4_RS25435 (ACK8N4_25435) - 5421941..5422630 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  ACK8N4_RS25440 (ACK8N4_25440) - 5422627..5423097 (-) 471 WP_003103866.1 hypothetical protein -
  ACK8N4_RS25445 (ACK8N4_25445) - 5423094..5423519 (-) 426 WP_033976385.1 GNAT family N-acetyltransferase -
  ACK8N4_RS25450 (ACK8N4_25450) - 5423652..5424281 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  ACK8N4_RS25455 (ACK8N4_25455) - 5424278..5424727 (+) 450 WP_024914662.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43983.83 Da        Isoelectric Point: 9.8103

>NTDB_id=1083226 ACK8N4_RS25410 WP_114232521.1 5418694..5419911(+) (pilC) [Pseudomonas aeruginosa strain DG-1]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPIDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTMMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1083226 ACK8N4_RS25410 WP_114232521.1 5418694..5419911(+) (pilC) [Pseudomonas aeruginosa strain DG-1]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAGAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TTTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCCATCGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCGCTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTTGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCGCAGTACTTCGACTCGCTCTACT
GCAACCTGGTAGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCGATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCAGCCTTCACCATGATGG
TCATCAACATTTCCAACGTACTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCTATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTGTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAATCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.519

100

0.785
  pilC Acinetobacter baylyi ADP1

61.029

100

0.615
  pilC Acinetobacter baumannii D1279779

60.396

99.753

0.602
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria gonorrhoeae MS11

46.04

99.753

0.459
  pilG Neisseria meningitidis 44/76-A

45.522

99.259

0.452
  pilC Vibrio cholerae strain A1552

41.481

100

0.415
  pilC Vibrio campbellii strain DS40M4

40.648

99.012

0.402
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment