Detailed information NTG1083225

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: ACK8N4_RS25405
• Genome accession: NZ_CP176811
• Coordinates: 5416990..5418690 (+)
• Length: 566 a.a.
• NCBI ID: WP_114232520.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain DG-1
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	5414464..5416348	5416990..5418690	flank	642

Gene organization within MGE regions

Location: 5414464..5418690

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACK8N4_RS25390 (ACK8N4_25390)	tnpB	5414464..5414823 (+)	360	WP_077473005.1	IS66 family insertion sequence element accessory protein TnpB	-
ACK8N4_RS25395 (ACK8N4_25395)	tnpC	5414843..5416348 (+)	1506	WP_121324304.1	IS66 family transposase	-
ACK8N4_RS25400 (ACK8N4_25400)	-	5416391..5416759 (-)	369	Protein_5024	pilin	-
ACK8N4_RS25405 (ACK8N4_25405)	pilB	5416990..5418690 (+)	1701	WP_114232520.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62315.26 Da
• Isoelectric Point: 5.4470

>NTDB_id=1083225 ACK8N4_RS25405 WP_114232520.1 5416990..5418690(+) (pilB) [Pseudomonas aeruginosa strain DG-1]
MNDSTLLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLGVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
ELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=1083225 ACK8N4_RS25405 WP_114232520.1 5416990..5418690(+) (pilB) [Pseudomonas aeruginosa strain DG-1]
ATGAACGATTCCACGCTTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATAGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAGCGCGGCAACAAACTGTTCATCGGCCTCTC
CGACCCGACCAACCACCAGGCGGTCACCGATATCCAGTTCAGCACCGGCCTCACCACCGAGGCCCTGCTGGTGGAGGACG
ACAAGCTCGGCGTAGCCATCGAGAAGCTCTACGAGGGTGCCATGGATGCCCTCGGCGATCTCGGCGACATCGATCTCGAC
GGGGTCGATGTGGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGACGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAGGTAGCCAAGCCGCCGATCCAGCTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGACATTTCCGAACGGCGTAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGACCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGAATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTGTACACCGGCCTGAACATCCTGAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTCACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAAGGTTTCCCCGAA
GAGCTGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

• Type II/IV secretion system protein (T2SSE) (190-457)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	57.544	100	0.58
pilB	Acinetobacter baylyi ADP1	56.94	99.293	0.565
pilB	Legionella pneumophila strain ERS1305867	54.497	100	0.546
pilB	Vibrio cholerae strain A1552	51.521	98.763	0.509
pilF	Neisseria gonorrhoeae MS11	50.177	99.647	0.5
pilB	Vibrio parahaemolyticus RIMD 2210633	52.158	94.17	0.491
pilB	Vibrio campbellii strain DS40M4	51.22	94.17	0.482
pilB/pilB1	Synechocystis sp. PCC 6803	37.48	100	0.41
pilF	Thermus thermophilus HB27	38.664	100	0.389
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.727	97.173	0.376