Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACLH0I_RS25090 Genome accession   NZ_CP176770
Coordinates   5769870..5771087 (+) Length   405 a.a.
NCBI ID   WP_411385656.1    Uniprot ID   -
Organism   Pseudomonas sp. MPB03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5764870..5776087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLH0I_RS25070 (ACLH0I_25070) - 5766294..5766590 (+) 297 WP_250964595.1 DUF2845 domain-containing protein -
  ACLH0I_RS25075 (ACLH0I_25075) - 5766902..5767255 (-) 354 WP_411385653.1 BON domain-containing protein -
  ACLH0I_RS25080 (ACLH0I_25080) - 5767526..5767942 (-) 417 WP_411385654.1 pilin -
  ACLH0I_RS25085 (ACLH0I_25085) pilB 5768167..5769867 (+) 1701 WP_411385655.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLH0I_RS25090 (ACLH0I_25090) pilC 5769870..5771087 (+) 1218 WP_411385656.1 type II secretion system F family protein Machinery gene
  ACLH0I_RS25095 (ACLH0I_25095) pilD 5771089..5771958 (+) 870 WP_411385657.1 prepilin peptidase Machinery gene
  ACLH0I_RS25100 (ACLH0I_25100) coaE 5771971..5772594 (+) 624 WP_411385658.1 dephospho-CoA kinase -
  ACLH0I_RS25105 (ACLH0I_25105) yacG 5772591..5772797 (+) 207 WP_186709040.1 DNA gyrase inhibitor YacG -
  ACLH0I_RS25110 (ACLH0I_25110) - 5772878..5773093 (-) 216 WP_053125334.1 hypothetical protein -
  ACLH0I_RS25115 (ACLH0I_25115) - 5773157..5773843 (-) 687 WP_411385659.1 energy-coupling factor ABC transporter permease -
  ACLH0I_RS25120 (ACLH0I_25120) - 5773863..5774276 (-) 414 WP_250964603.1 tautomerase family protein -
  ACLH0I_RS25125 (ACLH0I_25125) - 5774428..5775321 (+) 894 WP_411385660.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44300.06 Da        Isoelectric Point: 9.9843

>NTDB_id=1082839 ACLH0I_RS25090 WP_411385656.1 5769870..5771087(+) (pilC) [Pseudomonas sp. MPB03]
MAVKAVKTDVYTWEGKDRKGTKMTGELTGQSPALVKAQLRKQGINPEKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNPNMRKLVDTVKQEVAAGNSFAASLRKCPQYFDELYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPTAVILVAAVVTGILLVKVVPQFESVFSGFGAQLPAFTVMVIGLSEFMQEWWWMLLGGLAGAFFGVK
YALKRSQAFRDWRDKWLLKLPLIGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIRQDVSTGM
QLNFSMRASGIFPNLAIQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVILGIVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1082839 ACLH0I_RS25090 WP_411385656.1 5769870..5771087(+) (pilC) [Pseudomonas sp. MPB03]
ATGGCGGTCAAGGCAGTCAAAACCGATGTGTACACGTGGGAAGGCAAGGACCGCAAAGGCACGAAAATGACCGGTGAGCT
GACGGGCCAGAGCCCGGCACTGGTCAAGGCACAACTGCGCAAGCAAGGCATCAACCCGGAGAAGGTCCGCAAGAAATCCA
CCTCGATATTCAGCAAGGGCAAGCGCATCAAGCCGCTGGACATCGCCCTTTTCACCCGCCAGATGGCCACGATGCTCAAG
GCTGGCGTACCGCTGTTGCAAGCGTTCGACATCATCGGCGAAGGCTTCGACAACCCCAACATGCGTAAACTGGTGGATAC
GGTGAAGCAGGAAGTTGCCGCCGGCAACAGCTTCGCCGCCTCGCTGCGCAAATGCCCGCAGTATTTCGACGAGCTGTACT
GCAACCTGGTGGACGCCGGCGAACAGGCCGGTGCCCTGGATACGCTGCTGGACCGGGTGGCGACCTACAAGGAAAAGAGC
GAAGCCCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCGACGGCGGTGATACTCGTCGCAGCGGTGGTCACGGGCAT
CCTGCTGGTCAAGGTGGTGCCGCAGTTCGAATCGGTCTTCTCCGGGTTCGGGGCGCAGTTGCCGGCCTTCACGGTGATGG
TCATCGGCCTGTCGGAGTTCATGCAGGAGTGGTGGTGGATGCTGCTGGGCGGTTTGGCCGGCGCGTTTTTCGGGGTGAAA
TATGCCCTCAAGCGGTCCCAGGCGTTTCGTGACTGGCGGGACAAATGGCTACTCAAGCTCCCCTTGATCGGCACCTTGAT
GTACAAGTCCGCGGTGGCTCGCTTTGCCCGCACGCTCTCGACCACCTTCGCCGCCGGCGTGCCGCTGGTCGAAGCCCTCG
ATTCGGTGTCGGGCGCCACCGGCAACGTGGTATTCAAGCGCGCCGTGCAGCGCATCCGCCAGGACGTCTCCACCGGCATG
CAGTTGAATTTTTCCATGCGCGCGTCGGGGATCTTTCCGAACCTGGCCATCCAGATGACCGCCATCGGCGAGGAGTCCGG
CGCGCTGGACGACATGCTCGACAAGGTCGCGAGCTTTTATGAGGCCGAAGTGGACAATCTGGTGGACAACCTCACCAGCC
TGATGGAACCCTTCATCATGGTGATCCTGGGGATCGTCGTCGGTGGCCTGGTGGTTGCCATGTACTTGCCCATATTTCAA
CTCGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.827

100

0.738
  pilC Acinetobacter baylyi ADP1

60.302

98.272

0.593
  pilC Acinetobacter baumannii D1279779

58.088

100

0.585
  pilC Legionella pneumophila strain ERS1305867

56.061

97.778

0.548
  pilG Neisseria gonorrhoeae MS11

43.532

99.259

0.432
  pilG Neisseria meningitidis 44/76-A

43.035

99.259

0.427
  pilC Vibrio cholerae strain A1552

43.434

97.778

0.425
  pilC Vibrio campbellii strain DS40M4

42.929

97.778

0.42
  pilC Thermus thermophilus HB27

36.908

99.012

0.365

Multiple sequence alignment