Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACLH0I_RS25085 Genome accession   NZ_CP176770
Coordinates   5768167..5769867 (+) Length   566 a.a.
NCBI ID   WP_411385655.1    Uniprot ID   -
Organism   Pseudomonas sp. MPB03     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5763167..5774867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLH0I_RS25065 (ACLH0I_25065) - 5763359..5766292 (+) 2934 WP_411385652.1 DUF748 domain-containing protein -
  ACLH0I_RS25070 (ACLH0I_25070) - 5766294..5766590 (+) 297 WP_250964595.1 DUF2845 domain-containing protein -
  ACLH0I_RS25075 (ACLH0I_25075) - 5766902..5767255 (-) 354 WP_411385653.1 BON domain-containing protein -
  ACLH0I_RS25080 (ACLH0I_25080) - 5767526..5767942 (-) 417 WP_411385654.1 pilin -
  ACLH0I_RS25085 (ACLH0I_25085) pilB 5768167..5769867 (+) 1701 WP_411385655.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACLH0I_RS25090 (ACLH0I_25090) pilC 5769870..5771087 (+) 1218 WP_411385656.1 type II secretion system F family protein Machinery gene
  ACLH0I_RS25095 (ACLH0I_25095) pilD 5771089..5771958 (+) 870 WP_411385657.1 prepilin peptidase Machinery gene
  ACLH0I_RS25100 (ACLH0I_25100) coaE 5771971..5772594 (+) 624 WP_411385658.1 dephospho-CoA kinase -
  ACLH0I_RS25105 (ACLH0I_25105) yacG 5772591..5772797 (+) 207 WP_186709040.1 DNA gyrase inhibitor YacG -
  ACLH0I_RS25110 (ACLH0I_25110) - 5772878..5773093 (-) 216 WP_053125334.1 hypothetical protein -
  ACLH0I_RS25115 (ACLH0I_25115) - 5773157..5773843 (-) 687 WP_411385659.1 energy-coupling factor ABC transporter permease -
  ACLH0I_RS25120 (ACLH0I_25120) - 5773863..5774276 (-) 414 WP_250964603.1 tautomerase family protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62640.61 Da        Isoelectric Point: 5.3135

>NTDB_id=1082838 ACLH0I_RS25085 WP_411385655.1 5768167..5769867(+) (pilB) [Pseudomonas sp. MPB03]
MNDIALSGLTKQLVLAELLTEQSAQQAYQQAQRSRIPLVSYLVQNKLVQSRQIAEIASEHFGVALLDLNSLDKEAQPTGL
VSEKLVRQHHALPLWRRGNKLYVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDDTKQDSIGTQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKTYRVRVRTDGMLREVAKPPIQLATRIAARL
KVMASLDISERRKPQDGRLKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCNHCKKAIEVPEEALLKEGFPR
ERIGTFTIYEPVGCEQCNHGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRNDGFNDLRASGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1082838 ACLH0I_RS25085 WP_411385655.1 5768167..5769867(+) (pilB) [Pseudomonas sp. MPB03]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGCTGGCCGAACTGCTCACCGAGCAAAGTGCGCAACAGGC
GTATCAACAGGCCCAACGCAGCCGCATCCCCCTGGTCAGCTACCTGGTGCAGAACAAACTGGTCCAGAGCCGCCAGATCG
CGGAAATCGCCTCGGAGCATTTCGGCGTGGCCCTGCTGGACCTCAACAGCCTGGACAAGGAAGCCCAGCCCACCGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCACCACGCCCTGCCGCTGTGGCGGCGTGGCAACAAGCTGTACGTGGGCATCTCCGA
CCCTACCAATCACCAGGCCATCAACGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAATTCTTCGAGTCCAGTAGCACCGGCCTGGAAGGCATGGGCGATGTTGACCTCGATGGC
CTGGACATCGAATCGATCGACGACACCAAGCAGGACTCCATCGGCACCCAAGACACCGATGACGCGCCCGTGGTGCGCTT
CGTCAACAAGATGTTGCTGGACGCGATCAAGGGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAAAAAACCTACCGGG
TGCGGGTGCGCACCGACGGCATGCTGCGGGAAGTGGCCAAGCCGCCGATCCAATTGGCGACCCGCATCGCCGCCCGCCTG
AAGGTCATGGCCAGCCTCGATATTTCCGAGCGGCGCAAACCCCAGGACGGCCGGCTGAAAATGCGCCTGTCGAAAACCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAAAAAGTAGTGATCCGGATCCTCGACCCGTCCAGCG
CGCAAATGGGCATCGATGCCCTGGGCTACGAGCCAGACCAGAAAGACCTGTACATGGCCGCCCTCAAGCAGCCGCAAGGG
CTGATCCTGGTCACCGGCCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGGCTGAACATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTGAACCCGCGCCAGG
GGCTGGACTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAAATCGCCATCAAGGCCGCCCAGACGGGCCACCTGGTGCTGTCCACCCTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGCTTGCAGAACATGGGCATTCCGGGGTTCAACATCGCCACAGCCGTGCACCTGATCATCGCCCAGCGGC
TGGCGCGCAAGCTGTGCAACCACTGCAAGAAGGCCATCGAGGTTCCCGAGGAAGCCTTGCTCAAGGAAGGTTTCCCTCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACCACGGTTACAAAGGGCGCGTGGGGGT
TTACGAAGTGGTCAAGAACACGCCCGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATCTGCAGA
TGCGCAACGACGGCTTCAATGACCTGCGCGCATCGGGGCTGCTCAAAGTGATGCAGGGCGTCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.657

100

0.587
  pilB Acinetobacter baumannii D1279779

57.143

100

0.572
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.557
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio campbellii strain DS40M4

48.772

100

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

49.198

99.117

0.488
  pilF Neisseria gonorrhoeae MS11

47.957

99.47

0.477

Multiple sequence alignment