Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   ACK2JI_RS08545 Genome accession   NZ_CP176643
Coordinates   1736960..1737421 (+) Length   153 a.a.
NCBI ID   WP_002605736.1    Uniprot ID   -
Organism   [Clostridium] innocuum strain SFAD8     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1731491..1769970 1736960..1737421 within 0


Gene organization within MGE regions


Location: 1731491..1769970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK2JI_RS08500 - 1731491..1732180 (+) 690 WP_002605746.1 N-acetylmuramoyl-L-alanine amidase -
  ACK2JI_RS08505 - 1732379..1732774 (-) 396 WP_002605745.1 hypothetical protein -
  ACK2JI_RS08510 - 1733051..1733899 (-) 849 WP_002605744.1 tyrosine-type recombinase/integrase -
  ACK2JI_RS08525 - 1734423..1734791 (+) 369 WP_233105656.1 hypothetical protein -
  ACK2JI_RS08530 - 1735013..1735867 (+) 855 WP_002605740.1 hypothetical protein -
  ACK2JI_RS08535 - 1735873..1736145 (+) 273 WP_002611900.1 hypothetical protein -
  ACK2JI_RS08540 - 1736120..1736605 (+) 486 WP_002605738.1 class I SAM-dependent methyltransferase -
  ACK2JI_RS08545 ssb 1736960..1737421 (+) 462 WP_002605736.1 single-stranded DNA-binding protein Machinery gene
  ACK2JI_RS08550 - 1737439..1738224 (+) 786 WP_233105687.1 hypothetical protein -
  ACK2JI_RS08555 - 1738230..1738700 (+) 471 WP_002605734.1 helix-turn-helix transcriptional regulator -
  ACK2JI_RS08560 - 1738858..1739106 (+) 249 WP_002605732.1 hypothetical protein -
  ACK2JI_RS08565 - 1739093..1739341 (+) 249 WP_233105686.1 hypothetical protein -
  ACK2JI_RS08570 - 1739375..1739752 (+) 378 WP_002605730.1 hypothetical protein -
  ACK2JI_RS08575 - 1739754..1739948 (+) 195 WP_002605729.1 hypothetical protein -
  ACK2JI_RS08580 - 1739960..1741012 (+) 1053 WP_002605728.1 hypothetical protein -
  ACK2JI_RS08585 - 1741077..1741460 (+) 384 WP_002605727.1 hypothetical protein -
  ACK2JI_RS08590 - 1741530..1742138 (+) 609 WP_002605725.1 hypothetical protein -
  ACK2JI_RS08595 - 1742196..1742669 (+) 474 WP_008728434.1 hypothetical protein -
  ACK2JI_RS08600 - 1742660..1743421 (+) 762 WP_002605723.1 ParA family protein -
  ACK2JI_RS08605 - 1743414..1744286 (+) 873 WP_002605722.1 ParB/RepB/Spo0J family partition protein -
  ACK2JI_RS08610 - 1744283..1744576 (+) 294 WP_002605721.1 hypothetical protein -
  ACK2JI_RS08615 - 1744651..1745040 (+) 390 WP_002605720.1 hypothetical protein -
  ACK2JI_RS08620 - 1745777..1745965 (+) 189 WP_002605718.1 hypothetical protein -
  ACK2JI_RS08625 - 1745962..1746732 (+) 771 WP_233108867.1 hypothetical protein -
  ACK2JI_RS08630 - 1746835..1747461 (+) 627 WP_002605716.1 hypothetical protein -
  ACK2JI_RS08635 - 1747570..1747737 (+) 168 WP_156346883.1 hypothetical protein -
  ACK2JI_RS08640 - 1747939..1748526 (+) 588 WP_002605714.1 hypothetical protein -
  ACK2JI_RS08645 - 1748635..1749057 (+) 423 WP_002605713.1 hypothetical protein -
  ACK2JI_RS08650 - 1749233..1749613 (+) 381 WP_233105688.1 hypothetical protein -
  ACK2JI_RS08655 - 1749654..1750556 (+) 903 WP_002605711.1 Bro-N domain-containing protein -
  ACK2JI_RS08660 - 1750693..1751205 (+) 513 WP_002605710.1 ORF6N domain-containing protein -
  ACK2JI_RS08665 - 1751277..1751657 (+) 381 WP_038273999.1 hypothetical protein -
  ACK2JI_RS08670 - 1751693..1752640 (+) 948 WP_008728436.1 bacterial Ig-like domain-containing protein -
  ACK2JI_RS08675 - 1752854..1757263 (+) 4410 WP_142692098.1 SpaA isopeptide-forming pilin-related protein -
  ACK2JI_RS08680 - 1757272..1757919 (+) 648 WP_002611205.1 hypothetical protein -
  ACK2JI_RS08685 - 1758043..1758240 (+) 198 WP_002611201.1 hypothetical protein -
  ACK2JI_RS08690 dnaG 1758237..1760060 (+) 1824 WP_002611199.1 DNA primase -
  ACK2JI_RS08695 - 1760117..1760416 (+) 300 WP_002611197.1 hypothetical protein -
  ACK2JI_RS08700 - 1760429..1761256 (+) 828 WP_002611195.1 conjugal transfer protein TrbL family protein -
  ACK2JI_RS08705 - 1761261..1764086 (+) 2826 WP_002611193.1 VirB4 family type IV secretion system protein -
  ACK2JI_RS08710 - 1764086..1765465 (+) 1380 WP_002611190.1 peptidoglycan DD-metalloendopeptidase family protein -
  ACK2JI_RS08715 - 1765515..1768163 (+) 2649 WP_002611189.1 hypothetical protein -
  ACK2JI_RS08720 - 1768351..1769058 (+) 708 WP_002611187.1 HNH endonuclease -
  ACK2JI_RS08725 - 1769171..1769641 (+) 471 WP_002611186.1 hypothetical protein -
  ACK2JI_RS08730 - 1769638..1769970 (+) 333 WP_002611184.1 hypothetical protein -

Sequence


Protein


Download         Length: 153 a.a.        Molecular weight: 16780.62 Da        Isoelectric Point: 5.2136

>NTDB_id=1081859 ACK2JI_RS08545 WP_002605736.1 1736960..1737421(+) (ssb) [[Clostridium] innocuum strain SFAD8]
MINRVVLVGRLTKDPVLRKTTNGASVVSFTVACNRLFKQEGQPEADFINTVIWNKTADSVAKYTHKGSLVGVEGRIQTRS
YDDKDGKRVYVTEVVADNVRFLDSRNAGGTNSAYVPGQESMQSSTDTSNSYPSFSNEFTSIAALDISDDDLPF

Nucleotide


Download         Length: 462 bp        

>NTDB_id=1081859 ACK2JI_RS08545 WP_002605736.1 1736960..1737421(+) (ssb) [[Clostridium] innocuum strain SFAD8]
ATGATCAATAGAGTTGTATTAGTGGGAAGATTGACGAAGGACCCTGTATTACGGAAGACGACGAATGGCGCATCGGTCGT
GTCTTTTACGGTCGCATGTAACCGGCTTTTCAAACAGGAGGGACAGCCAGAAGCTGACTTCATCAACACTGTCATCTGGA
ACAAGACCGCAGACAGTGTAGCAAAGTATACGCACAAGGGCTCTCTGGTAGGTGTAGAGGGCCGTATCCAAACACGCAGT
TATGATGACAAAGACGGCAAACGTGTATATGTAACAGAGGTGGTCGCAGATAATGTGCGCTTTTTGGATAGTAGGAATGC
GGGAGGTACGAACAGTGCCTACGTACCAGGACAGGAAAGCATGCAGAGCAGCACTGATACGAGCAACAGCTATCCATCCT
TCTCCAATGAGTTCACGAGTATTGCAGCGCTGGATATCTCCGATGACGATCTGCCATTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

47.647

100

0.529

  ssbA Bacillus subtilis subsp. subtilis str. 168

45.143

100

0.516

  ssbB Bacillus subtilis subsp. subtilis str. 168

59.434

69.281

0.412

  ssbB/cilA Streptococcus mitis NCTC 12261

38.312

100

0.386

  ssbB/cilA Streptococcus pneumoniae TIGR4

37.662

100

0.379

  ssbB/cilA Streptococcus pneumoniae Rx1

37.013

100

0.373

  ssbB/cilA Streptococcus pneumoniae D39

37.013

100

0.373

  ssbB/cilA Streptococcus pneumoniae R6

37.013

100

0.373

  ssbB/cilA Streptococcus mitis SK321

37.013

100

0.373

  ssb Neisseria meningitidis MC58

32.37

100

0.366

  ssb Neisseria gonorrhoeae MS11

32.37

100

0.366


Multiple sequence alignment