Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   AACH55_RS01635 Genome accession   NZ_AP029028
Coordinates   366746..367882 (-) Length   378 a.a.
NCBI ID   WP_338717665.1    Uniprot ID   -
Organism   Herbaspirillum sp. DW155     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 361746..372882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH55_RS01605 (HBDW_03160) - 362293..362718 (+) 426 WP_338717658.1 NUDIX domain-containing protein -
  AACH55_RS01610 (HBDW_03170) yacG 362761..362946 (-) 186 WP_034336666.1 DNA gyrase inhibitor YacG -
  AACH55_RS01615 (HBDW_03180) zapD 362983..363738 (-) 756 WP_338717661.1 cell division protein ZapD -
  AACH55_RS01620 (HBDW_03190) coaE 363954..364574 (-) 621 WP_338717663.1 dephospho-CoA kinase -
  AACH55_RS01625 (HBDW_03200) pilD 364578..365441 (-) 864 WP_338720135.1 A24 family peptidase Machinery gene
  AACH55_RS01630 (HBDW_03210) - 365503..366759 (-) 1257 WP_338717664.1 type II secretion system F family protein -
  AACH55_RS01635 (HBDW_03220) pilF 366746..367882 (-) 1137 WP_338717665.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  AACH55_RS01640 (HBDW_03230) - 368035..369327 (-) 1293 WP_338717666.1 HlyC/CorC family transporter -
  AACH55_RS01645 (HBDW_03240) - 369487..370455 (-) 969 WP_338717667.1 glyoxylate/hydroxypyruvate reductase A -
  AACH55_RS01650 (HBDW_03250) - 370452..371309 (-) 858 WP_338717668.1 IclR family transcriptional regulator -
  AACH55_RS01655 (HBDW_03260) - 371575..372660 (+) 1086 WP_338717670.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 40702.24 Da        Isoelectric Point: 8.2661

>NTDB_id=108185 AACH55_RS01635 WP_338717665.1 366746..367882(-) (pilF) [Herbaspirillum sp. DW155]
MQAPDTAVADDDVPVVRLLHKLLADAVGMGASDLHFEPFEHLFRVRLRVDGVLHELAQPPLSLRDKLATRLKILARLDIA
EKRLPQDGKMRLALGARTVDFRVSTLPTQFGEKIVLRILDTTQAALSIAELGYEPAQREALLQAISKPHGLVLMTGPTGS
GKTVSLYACLQRLNQPGVNIATAEDPVEINLPGINQVSINERAGLDFALALRAFLRQDPDVLMVGEIRDQETADIAVKAS
QTGHLVLSTLHTNDAPATLTRLLNMGVPAYNIAASVSLIVAQRLVRRLCHCRRPAGGTSASFVPHGCPACNQTGFRGRTG
IYQVMPVSAAMQSLILAQAGTLELGRQAQAEGIIDLRRAGLLKVLRGETSLGEILACT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=108185 AACH55_RS01635 WP_338717665.1 366746..367882(-) (pilF) [Herbaspirillum sp. DW155]
ATGCAAGCTCCCGACACGGCCGTGGCCGACGACGATGTTCCGGTGGTGCGCCTGCTGCACAAGCTGCTGGCCGATGCGGT
CGGCATGGGCGCCTCCGACCTGCATTTCGAACCCTTTGAACATCTCTTCCGTGTGCGCCTGCGTGTGGATGGCGTTCTGC
ACGAGCTGGCGCAGCCGCCGCTGAGCCTGCGCGACAAGCTGGCCACGCGCCTGAAGATCCTGGCGCGGCTGGATATCGCC
GAAAAGCGCCTGCCCCAGGACGGCAAGATGCGCCTGGCGCTGGGCGCACGCACGGTGGACTTCCGCGTCTCGACGCTGCC
CACGCAGTTCGGCGAAAAGATCGTGCTGCGCATCCTCGATACCACCCAGGCCGCCTTGTCGATTGCCGAGCTGGGTTATG
AGCCGGCGCAGCGGGAAGCGCTGCTGCAGGCCATCAGCAAGCCGCACGGCCTGGTGCTGATGACCGGGCCGACCGGCTCG
GGCAAGACGGTCTCGCTGTATGCCTGCCTGCAGCGCCTGAACCAGCCGGGCGTAAACATCGCCACCGCCGAGGATCCGGT
GGAGATCAATCTGCCCGGCATCAACCAGGTCAGCATCAACGAACGCGCGGGACTGGATTTCGCGCTGGCGCTGCGCGCCT
TCCTGCGCCAGGACCCGGACGTGCTGATGGTGGGCGAGATCCGCGACCAGGAAACCGCCGACATCGCCGTCAAGGCCTCG
CAGACCGGGCATCTGGTGCTCTCCACCCTGCACACCAACGACGCTCCGGCCACGCTCACGCGGCTGCTCAACATGGGCGT
GCCGGCCTACAACATCGCTGCCTCGGTCAGCCTGATCGTGGCCCAGCGGCTGGTGCGCAGGCTGTGCCACTGCCGTCGTC
CGGCCGGTGGCACGTCGGCCAGCTTCGTGCCGCATGGCTGCCCGGCCTGCAACCAGACCGGCTTTCGCGGACGCACCGGG
ATCTACCAGGTCATGCCGGTCAGCGCCGCCATGCAATCGCTCATCCTGGCCCAGGCCGGCACGCTGGAGCTGGGCCGGCA
GGCACAGGCTGAAGGCATCATCGACTTGCGTCGTGCGGGACTGCTGAAGGTCTTGCGCGGCGAAACCAGCCTGGGAGAGA
TCCTTGCCTGCACCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(22-287)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

57.069

100

0.587
  pilB Acinetobacter baylyi ADP1

55.065

100

0.561
  pilB Acinetobacter baumannii D1279779

54.639

100

0.561
  pilB Vibrio cholerae strain A1552

54.308

100

0.55
  pilB Vibrio campbellii strain DS40M4

54.45

100

0.55
  pilB Vibrio parahaemolyticus RIMD 2210633

53.665

100

0.542
  pilB Legionella pneumophila strain ERS1305867

50.891

100

0.529
  pilB Haemophilus influenzae 86-028NP

47.554

97.354

0.463
  pilB Haemophilus influenzae Rd KW20

47.283

97.354

0.46
  pilF Thermus thermophilus HB27

44.156

100

0.45
  pilB/pilB1 Synechocystis sp. PCC 6803

41.872

100

0.45
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.785

100

0.447
  pilB Glaesserella parasuis strain SC1401

48.742

84.127

0.41
  ctsE Campylobacter jejuni subsp. jejuni 81-176

39.37

100

0.397

Multiple sequence alignment