Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACJA3S_RS03285 Genome accession   NZ_CP174199
Coordinates   711875..712285 (+) Length   136 a.a.
NCBI ID   WP_406820819.1    Uniprot ID   -
Organism   Pseudomonas sp. KnCO4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 706875..717285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJA3S_RS03255 (ACJA3S_03255) - 707065..707694 (-) 630 WP_013970766.1 DUF1780 domain-containing protein -
  ACJA3S_RS03260 (ACJA3S_03260) - 707886..708569 (+) 684 WP_406820815.1 energy-coupling factor ABC transporter permease -
  ACJA3S_RS03265 (ACJA3S_03265) yacG 708761..708961 (-) 201 WP_170028160.1 DNA gyrase inhibitor YacG -
  ACJA3S_RS03270 (ACJA3S_03270) coaE 708958..709581 (-) 624 WP_406820816.1 dephospho-CoA kinase -
  ACJA3S_RS03275 (ACJA3S_03275) pilD 709581..710447 (-) 867 WP_406820817.1 prepilin peptidase Machinery gene
  ACJA3S_RS03280 (ACJA3S_03280) - 710450..711658 (-) 1209 WP_406820818.1 type II secretion system F family protein -
  ACJA3S_RS03285 (ACJA3S_03285) pilA 711875..712285 (+) 411 WP_406820819.1 pilin Machinery gene
  ACJA3S_RS03295 (ACJA3S_03295) - 712592..712708 (+) 117 Protein_630 Arm DNA-binding domain-containing protein -
  ACJA3S_RS03300 (ACJA3S_03300) - 713317..714171 (-) 855 WP_406820820.1 hypothetical protein -
  ACJA3S_RS03305 (ACJA3S_03305) - 714431..715138 (-) 708 WP_406820821.1 hypothetical protein -
  ACJA3S_RS03310 (ACJA3S_03310) - 715279..715992 (+) 714 WP_406820822.1 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13798.14 Da        Isoelectric Point: 7.0814

>NTDB_id=1074957 ACJA3S_RS03285 WP_406820819.1 711875..712285(+) (pilA) [Pseudomonas sp. KnCO4]
MKGQRGITLIELMIVVAIIGILATIALPMYTNHQSRSKAAAGLLEISALKTAMDLRLNDGKDVATVAELGGQPATAHCAI
TATGKAADGTGSIVCTLVDAPATVLGKAITLTRAAAGWACTTDIEEDLAPKGCKAP

Nucleotide


Download         Length: 411 bp        

>NTDB_id=1074957 ACJA3S_RS03285 WP_406820819.1 711875..712285(+) (pilA) [Pseudomonas sp. KnCO4]
ATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTTGTCGCGATCATCGGCATTCTGGCGACCATCGCCTT
ACCGATGTACACCAACCACCAGTCCCGCTCCAAGGCTGCGGCCGGGTTGCTGGAAATCAGCGCGCTGAAGACGGCGATGG
ACCTGCGCCTGAATGATGGCAAGGACGTGGCGACGGTGGCCGAGCTGGGTGGGCAGCCGGCCACGGCGCATTGTGCGATC
ACGGCCACCGGCAAGGCGGCGGACGGCACCGGGAGCATCGTTTGTACCCTGGTGGACGCGCCGGCTACCGTGCTCGGCAA
GGCCATTACCCTGACGCGCGCGGCGGCTGGCTGGGCGTGTACGACCGACATTGAAGAAGACTTGGCACCGAAGGGGTGCA
AGGCGCCTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

45.27

100

0.493
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.704

99.265

0.434
  pilA Acinetobacter baumannii strain A118

42.446

100

0.434
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.647

100

0.426
  comP Acinetobacter baylyi ADP1

38

100

0.419
  pilA Vibrio cholerae C6706

39.86

100

0.419
  pilA Vibrio cholerae strain A1552

39.86

100

0.419
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.86

100

0.419
  pilA Vibrio parahaemolyticus RIMD 2210633

40.984

89.706

0.368

Multiple sequence alignment