Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACI514_RS29895 Genome accession   NZ_CP173611
Coordinates   6689161..6690378 (-) Length   405 a.a.
NCBI ID   WP_160059459.1    Uniprot ID   A0A7Z2KXJ7
Organism   Pseudomonas sp. M20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6684161..6695378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI514_RS29855 (ACI514_29845) - 6684946..6685119 (-) 174 WP_007909875.1 DUF3094 domain-containing protein -
  ACI514_RS29860 (ACI514_29850) - 6685171..6685698 (-) 528 WP_160059464.1 MOSC domain-containing protein -
  ACI514_RS29865 (ACI514_29855) - 6685695..6686321 (-) 627 WP_007909887.1 DUF1780 domain-containing protein -
  ACI514_RS29870 (ACI514_29860) - 6686494..6687183 (+) 690 WP_405122853.1 energy-coupling factor ABC transporter permease -
  ACI514_RS29875 (ACI514_29865) - 6687249..6687464 (+) 216 WP_038369048.1 hypothetical protein -
  ACI514_RS29880 (ACI514_29870) yacG 6687470..6687670 (-) 201 WP_160059462.1 DNA gyrase inhibitor YacG -
  ACI514_RS29885 (ACI514_29875) coaE 6687667..6688290 (-) 624 WP_160059461.1 dephospho-CoA kinase -
  ACI514_RS29890 (ACI514_29880) pilD 6688287..6689159 (-) 873 WP_160059460.1 A24 family peptidase Machinery gene
  ACI514_RS29895 (ACI514_29885) pilC 6689161..6690378 (-) 1218 WP_160059459.1 type II secretion system F family protein Machinery gene
  ACI514_RS29900 (ACI514_29890) pilB 6690381..6692081 (-) 1701 WP_405122854.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACI514_RS29905 (ACI514_29895) - 6692307..6692729 (+) 423 WP_160059457.1 pilin -
  ACI514_RS29910 (ACI514_29900) - 6693027..6693383 (+) 357 WP_405122855.1 BON domain-containing protein -
  ACI514_RS29915 (ACI514_29905) - 6693655..6693951 (-) 297 WP_405122856.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44166.91 Da        Isoelectric Point: 10.1093

>NTDB_id=1073173 ACI514_RS29895 WP_160059459.1 6689161..6690378(-) (pilC) [Pseudomonas sp. M20]
MAVKAAKISIYAWEGTDRKGSKVTGELSGQNPALIKAQLRKQGINPGKVRKKTASLLSFGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
ERLKAKIKKAMTYPTAVVLVAAVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVISLSEFMQQWWWAILGALVVTIFGTR
HALKTSQALRDRKDTWLLKLPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDEMLDKVAGFYEEEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1073173 ACI514_RS29895 WP_160059459.1 6689161..6690378(-) (pilC) [Pseudomonas sp. M20]
ATGGCGGTCAAGGCAGCGAAAATCAGCATTTATGCCTGGGAAGGCACGGACAGGAAAGGCAGCAAAGTCACTGGCGAATT
AAGTGGACAGAACCCTGCACTGATCAAGGCACAACTGCGTAAACAGGGGATCAATCCCGGCAAAGTACGCAAGAAGACCG
CGTCGTTGTTGAGCTTTGGCAAACGCATCAAAGCCCAGGACATTGCTCTGTTCACCCGGCAGATGGCGACCATGATGAAG
GCTGGTGTGCCGCTGCTGCAGTCGTTCGACATCATTGGCGAAGGCTTTGAAAACCCGGCCATGCGCAAACTGGTGGACGA
GGTGAAGCAGGAGGTCGCGGCCGGTAACAGCTTCGCCACAGCCCTGCGCAAGAAGCCGCAGTATTTCGACGAGTTGTATT
GCAATCTGGTCGACGCCGGCGAGCAGTCCGGCGCACTCGACACGTTGCTGGAACGGGTCGCGACCTATAAGGAAAAAAGC
GAACGGCTCAAGGCCAAGATCAAAAAAGCCATGACCTACCCCACCGCCGTGGTGCTGGTGGCAGCAGTCGTGACCGGAAT
ACTGCTGGTTAAAGTGGTGCCGCAGTTTCAGTCGGTGTTTTCCGGTTTCGGCGCAGAGCTGCCGGCCTTCACGCTGATGG
TGATCAGTCTGTCGGAATTCATGCAGCAATGGTGGTGGGCGATTCTCGGCGCGCTGGTCGTGACGATCTTTGGTACTCGC
CATGCACTGAAAACATCTCAGGCATTACGCGATCGCAAAGACACCTGGCTATTGAAACTGCCCTTGGTGGGCACGCTGAT
GTACAAATCCGCCGTGGCGCGATTCGCTCGAACCCTGTCGACCACATTCGCTGCCGGTGTGCCGCTGGTGGAAGCGCTCG
ACTCGGTGGCCGGCGCCACCGGCAACGTGGTGTTCAAGCGTGCTGTGCTGCGGATTCGTCAGGACGTCTCGACCGGCATG
CAGCTGAATTTTTCGATGCGCAGCACCGGGGTCTTTCCCAACATGGCCGTGCAAATGACCGCGATCGGCGAAGAGTCCGG
CGCGCTGGATGAGATGCTCGACAAGGTCGCGGGGTTTTACGAAGAGGAAGTGGATAACATGGTCGACAACCTCACCAGCC
TTATGGAGCCATTTATCATGGTGGTGCTGGGGGTAATCGTCGGTGGGCTGGTGGTGGCCATGTATTTGCCGATCTTCCAA
CTTGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2KXJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.074

100

0.741
  pilC Acinetobacter baumannii D1279779

59.314

100

0.598
  pilC Acinetobacter baylyi ADP1

58.824

100

0.593
  pilC Legionella pneumophila strain ERS1305867

54.04

97.778

0.528
  pilG Neisseria gonorrhoeae MS11

43.424

99.506

0.432
  pilC Vibrio cholerae strain A1552

43.687

97.778

0.427
  pilG Neisseria meningitidis 44/76-A

42.928

99.506

0.427
  pilC Vibrio campbellii strain DS40M4

41.133

100

0.412
  pilC Thermus thermophilus HB27

38.653

99.012

0.383

Multiple sequence alignment