Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACI514_RS29900 Genome accession   NZ_CP173611
Coordinates   6690381..6692081 (-) Length   566 a.a.
NCBI ID   WP_405122854.1    Uniprot ID   -
Organism   Pseudomonas sp. M20     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6685381..6697081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI514_RS29865 (ACI514_29855) - 6685695..6686321 (-) 627 WP_007909887.1 DUF1780 domain-containing protein -
  ACI514_RS29870 (ACI514_29860) - 6686494..6687183 (+) 690 WP_405122853.1 energy-coupling factor ABC transporter permease -
  ACI514_RS29875 (ACI514_29865) - 6687249..6687464 (+) 216 WP_038369048.1 hypothetical protein -
  ACI514_RS29880 (ACI514_29870) yacG 6687470..6687670 (-) 201 WP_160059462.1 DNA gyrase inhibitor YacG -
  ACI514_RS29885 (ACI514_29875) coaE 6687667..6688290 (-) 624 WP_160059461.1 dephospho-CoA kinase -
  ACI514_RS29890 (ACI514_29880) pilD 6688287..6689159 (-) 873 WP_160059460.1 A24 family peptidase Machinery gene
  ACI514_RS29895 (ACI514_29885) pilC 6689161..6690378 (-) 1218 WP_160059459.1 type II secretion system F family protein Machinery gene
  ACI514_RS29900 (ACI514_29890) pilB 6690381..6692081 (-) 1701 WP_405122854.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACI514_RS29905 (ACI514_29895) - 6692307..6692729 (+) 423 WP_160059457.1 pilin -
  ACI514_RS29910 (ACI514_29900) - 6693027..6693383 (+) 357 WP_405122855.1 BON domain-containing protein -
  ACI514_RS29915 (ACI514_29905) - 6693655..6693951 (-) 297 WP_405122856.1 DUF2845 domain-containing protein -
  ACI514_RS29920 (ACI514_29910) - 6693951..6696902 (-) 2952 WP_405122857.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62710.69 Da        Isoelectric Point: 5.9192

>NTDB_id=1073174 ACI514_RS29900 WP_405122854.1 6690381..6692081(-) (pilB) [Pseudomonas sp. M20]
MNDIALSGLTKQLVQAELLTEKGAQQAWQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHTSGLEDMADVDLDG
LDVESIDDSKQEAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPVQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDIQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1073174 ACI514_RS29900 WP_405122854.1 6690381..6692081(-) (pilB) [Pseudomonas sp. M20]
ATGAATGACATCGCTCTGAGCGGTCTGACCAAACAATTGGTGCAGGCCGAACTGCTCACGGAAAAAGGCGCCCAGCAAGC
CTGGCAACAGGCCCAGCGCAATCGCCTGTCGCTGGTCAGCTATCTGGTGCAGAACAAACTGGTGAAAAGCTCACAGGTCG
CCGAGATCGCTTCCGAGCACTTCGGCATGGCGTTCCTCGATCTCAACTGTCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGCGAAAAACTCGTACGCCAGCATCACGCCCTGCCTTTGTGGCGACGCGGCAACAAGCTGTTCGTGGGGATTTCCGA
CCCGAGCAATCATCAAGCGATCAACGACATTCAGTTCAGTACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AGCTCACCGATGCCATCGAGAAGTTCTTCGACACCCACACCAGTGGCCTGGAAGACATGGCCGATGTCGATCTCGATGGC
CTCGATGTCGAATCAATCGACGACAGCAAACAGGAGGCCATCGGTGGCCTCGATGCCGACGATGCGCCGGTGGTGCGTTT
CGTTCACAAGATGCTGCTAGACGCGATCAAGAGCGGCTCATCCGACTTGCACTTCGAACCTTACGAGAAGAACTACCGGG
TGCGGGTGCGCACCGACGGCATCCTGCGCGAGGTGGCCAAGCCACCGGTTCAACTGGCCGGGCGCATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTCGATATCTCGGAACGGCGCAAACCGCAGGACGGGCGGATCAAGATGCGTCTGTCGAAGAGCAA
GTCGATCGATTTCCGCGTCAACACCTTGCCGACCCTGTGGGGCGAGAAAGTGGTGATCCGGATCCTCGACCCGTCCAGTG
CGCAGATCGGCATCGACGCCCTTGGCTATGAACCAGAGCAGAAAGACCTGTACATGGCCGCGCTCAAGCAGCCACAGGGC
ATGATTCTGGTCACCGGGCCCACCGGATCGGGTAAAACCGTGTCGCTGTACACCGGCCTGAATATCCTCAACACCGTCGA
CATCAACATCTCCACCGCTGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAACAAG
GACTCGACTTTGCTCAGGCGCTGCGCTCGTTTCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGATCTG
GAAACCGCCGAGATCGCGATCAAGGCTGCGCAGACCGGTCACCTGGTGCTCTCGACCCTGCATACCAACAGCGCCGCGGA
AACTCTGACGCGCCTGCATAACATGGGCATTCCCGGTTTCAACATCGCCACGTCGGTCAGTCTGATCATCGCCCAGCGCC
TGGCGCGCAAACTGTGCAGCCACTGCAAGAAGCCCATCGAGATCCCACGCGAAACTTTGATCAAGGAAGGCTTCCCCGAG
GAACGCATCGGTCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGCGGTTACAAGGGACGCGTGGGGAT
TTATGAAGTGGTGAAAAACACACCGGAGCTGCAACGCTTGATCATGGCCGAAGGCAACTCGCTGGAAATCGACATTCAAA
TGCGCCGCGACGGCTTCGACGACCTGCGCACCTCCGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.688

99.647

0.585
  pilB Acinetobacter baylyi ADP1

58.437

99.47

0.581
  pilB Legionella pneumophila strain ERS1305867

55.752

99.823

0.557
  pilB Vibrio cholerae strain A1552

51.15

99.823

0.511
  pilB Vibrio parahaemolyticus RIMD 2210633

49.735

100

0.498
  pilB Vibrio campbellii strain DS40M4

49.123

100

0.495
  pilF Neisseria gonorrhoeae MS11

49.556

99.47

0.493
  pilF Thermus thermophilus HB27

36.991

99.823

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.826

93.286

0.362

Multiple sequence alignment