Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACI3L6_RS12345 Genome accession   NZ_CP173383
Coordinates   2774529..2776271 (-) Length   580 a.a.
NCBI ID   WP_050906101.1    Uniprot ID   A0A812GW56
Organism   Vibrio campbellii strain LB314     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2769529..2781271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3L6_RS12325 (ACI3L6_12325) - 2770234..2771073 (-) 840 WP_038889578.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  ACI3L6_RS12330 (ACI3L6_12330) - 2771143..2772636 (-) 1494 WP_038889579.1 SPOR domain-containing protein -
  ACI3L6_RS12335 (ACI3L6_12335) aroB 2772690..2773790 (-) 1101 WP_045380637.1 3-dehydroquinate synthase -
  ACI3L6_RS12340 (ACI3L6_12340) aroK 2773822..2774340 (-) 519 WP_010443904.1 shikimate kinase AroK -
  ACI3L6_RS12345 (ACI3L6_12345) pilQ 2774529..2776271 (-) 1743 WP_050906101.1 type IV pilus secretin PilQ Machinery gene
  ACI3L6_RS12350 (ACI3L6_12350) pilP 2776306..2776821 (-) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  ACI3L6_RS12355 (ACI3L6_12355) pilO 2776811..2777404 (-) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACI3L6_RS12360 (ACI3L6_12360) pilN 2777397..2777972 (-) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  ACI3L6_RS12365 (ACI3L6_12365) pilM 2777956..2778972 (-) 1017 WP_005432712.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 63615.59 Da        Isoelectric Point: 4.5366

>NTDB_id=1071826 ACI3L6_RS12345 WP_050906101.1 2774529..2776271(-) (pilQ) [Vibrio campbellii strain LB314]
MKMRHGLTLTPRKLTGLFSLWFFLLLAPFVAAEEVQSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYSHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEKEIGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGEGDDGGSSVGIDDFLNVNLAATSPNATS
IAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQIT
PDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRRS
YENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=1071826 ACI3L6_RS12345 WP_050906101.1 2774529..2776271(-) (pilQ) [Vibrio campbellii strain LB314]
ATGAAAATGAGACACGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTCTTTCTACTTTTAGC
CCCTTTCGTCGCAGCAGAAGAAGTACAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACGAACAAAGACAAAGACGCCG
TGATTGTGGTTGAGCTCGCATCACCCGCTGCGATTGTTGATATTAAACGCGTACAAGAAGGCTTGAGTATCGACTTACTC
AACACCTCCGTTAAAGATGACCAACTTTACCTACTTGATGTGAAAGATTTCTCGACGGTTGTCGAGAGCGTTGAGGTATT
CCGCGATACTTCTACAACACGTCTGGTGGCTTATATCGATGAGGACTATTCTCACGACTACCGTCTTACTGGTCGTTATT
TAGAGGTAAAGGTTGGCAAGCTTAAAGCCAACGAAAAAGCGCCGGATAAAAGCATTTTGGAAAAAGAAGGCAAGCTAATT
TCGATTAACTTCCAAGACATTCCTGTGCGAAACGTGTTGCAACTTATCGCCGACTACAATCAATTTAACTTGGTCGTCTC
TGACTCGGTCGAGGGTAACTTGACCTTGCGTTTGGATGGCGTGCCTTGGCAGCAAGTACTCGACATTATCTTGCAAGTGA
AAGGGTTAGATAAGCGTGTTGATGGCAATGTCATTCTCGTTGCACCAAAAGATGAATTGGATCTGCGCGAGAAGCAGCAA
CTCGAAAAGCAAAAGCTAGAGAAAGAGATCGGTGAGCTTTCATCAGAGATCATCAAGGTGAACTTCGCTAAAGCGTCAGA
CATTGCTGAGATGATCAATGGGGAGGGCAACATCAGTATGCTTTCTGATCGAGGCAGCATGACCATTGATGAGCGAACAA
ACTCGTTATTGATTCGTGAGTTACCTGAGAATATTGAAGTGATTCGCGAGATCATAGAATCATTGGATATTCCTGTAAAA
CAGGTTCAGATTGAAGCGCGTATTGTGACTATTAATGAAGGTAACATGGACGAACTTGGCGTTCGTTGGGGCTTCACTTC
CATCAATGGCAGTCATACTGTAGGTGGTTCAATTGAGAATAACCTTGCGACAATCGGCCTGTACGATGGTGGCGGTGAAG
GTGATGACGGTGGCAGCAGTGTGGGTATTGATGACTTTCTCAACGTTAACCTTGCCGCGACCAGTCCAAATGCGACCAGT
ATTGCCTTCCAAGTTGCGAAGTTAGGTTCGGACACCTTACTAGATTTGGAACTTTCGGCGCTGCAACAAGAATCGAAAGC
AGAAATTATTTCTAGTCCACGTTTGATTACCACGAATAAGAAGCCAGCTTACATTGAGCAGGGTACCGAGATCCCTTACC
TTGAATCTTCTTCCAGTGGTGCGACTTCTGTGACGTTCAAAAAAGCGGTATTGAGCTTGAAAGTGACGCCACAGATTACG
CCTGACAATCGTTTGGTGTTGGATTTGAGCGTTACGCAAGACAGACCGGGCCAAGTGGTAAAAACAGGCACTGGTGAGGC
GGTGGCGATCGATACGCAACGTATTGGTACACAAGTTTTAGTCAATAATGGCGAGACAGTCGTGTTGGGCGGTATTTTTC
AACACAGCATTACAAACTCCGTCGATAAGGTGCCTTTATTAGGTGATTTGCCGCTGTTAGGTGCGCTTTTCCGTCGAAGT
TACGAAAATGTCGGTAAAAGTGAACTACTGATTTTTGTTACACCTAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(420-578)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A812GW56

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

99.483

100

0.995
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.739

97.586

0.759
  pilQ Vibrio cholerae strain A1552

77.739

97.586

0.759
  pilQ Legionella pneumophila strain Lp02

41.894

94.655

0.397
  pilQ Legionella pneumophila strain ERS1305867

41.894

94.655

0.397
  pilQ Pseudomonas aeruginosa PAK

40.354

97.414

0.393
  pilQ Acinetobacter baumannii D1279779

37.521

100

0.386
  pilQ Acinetobacter baumannii strain A118

37.521

100

0.386
  comQ Acinetobacter baylyi ADP1

36.788

99.828

0.367

Multiple sequence alignment