Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACI3L6_RS12360 Genome accession   NZ_CP173383
Coordinates   2777397..2777972 (-) Length   191 a.a.
NCBI ID   WP_005432710.1    Uniprot ID   -
Organism   Vibrio campbellii strain LB314     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2772397..2782972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3L6_RS12335 (ACI3L6_12335) aroB 2772690..2773790 (-) 1101 WP_045380637.1 3-dehydroquinate synthase -
  ACI3L6_RS12340 (ACI3L6_12340) aroK 2773822..2774340 (-) 519 WP_010443904.1 shikimate kinase AroK -
  ACI3L6_RS12345 (ACI3L6_12345) pilQ 2774529..2776271 (-) 1743 WP_050906101.1 type IV pilus secretin PilQ Machinery gene
  ACI3L6_RS12350 (ACI3L6_12350) pilP 2776306..2776821 (-) 516 WP_005432702.1 pilus assembly protein PilP Machinery gene
  ACI3L6_RS12355 (ACI3L6_12355) pilO 2776811..2777404 (-) 594 WP_005432718.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACI3L6_RS12360 (ACI3L6_12360) pilN 2777397..2777972 (-) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  ACI3L6_RS12365 (ACI3L6_12365) pilM 2777956..2778972 (-) 1017 WP_005432712.1 type IV pilus biogenesis protein PilM Machinery gene
  ACI3L6_RS12370 (ACI3L6_12370) - 2779151..2781718 (+) 2568 WP_047482703.1 penicillin-binding protein 1A -
  ACI3L6_RS12375 (ACI3L6_12375) oxyR 2781796..2782704 (-) 909 WP_005432705.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22194.53 Da        Isoelectric Point: 9.4267

>NTDB_id=1071829 ACI3L6_RS12360 WP_005432710.1 2777397..2777972(-) (pilN) [Vibrio campbellii strain LB314]
MLYHINLLAWRENQREEHRRRFIGLILLGAIAALGVQWGIGKYYQYQQDQQQQRLDYLAHYISQLDKRIEAMKIAEQEHS
KILERLKTVEGLQNGRNKTTEFMNLMPSVIPEGVYVDKIKMNDYEIEISGISDSTSRLATMLDNMERSAKLLDVDMHSIV
HGKARFGKEFQTFKVSFMFKDASIKGGKQHG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=1071829 ACI3L6_RS12360 WP_005432710.1 2777397..2777972(-) (pilN) [Vibrio campbellii strain LB314]
ATGCTGTATCACATTAACCTTCTTGCCTGGCGTGAAAACCAACGTGAAGAGCATCGTCGTCGTTTTATTGGCTTGATTTT
ACTCGGGGCGATCGCTGCTCTGGGCGTGCAATGGGGGATCGGTAAGTACTACCAATACCAACAAGATCAGCAGCAACAGC
GCTTGGATTATCTCGCTCACTATATCTCTCAACTCGATAAACGTATTGAAGCCATGAAGATAGCAGAGCAAGAGCACAGC
AAAATTCTAGAGCGCTTAAAGACCGTTGAGGGGTTGCAAAATGGGCGCAATAAAACCACTGAGTTTATGAACCTTATGCC
CTCAGTGATCCCTGAAGGCGTCTATGTCGATAAGATCAAAATGAACGACTACGAGATTGAGATCTCGGGTATTTCTGATA
GCACCTCTCGTCTTGCCACCATGCTCGATAATATGGAGCGTTCTGCCAAGCTTCTGGATGTTGATATGCACTCCATTGTC
CATGGTAAGGCGCGCTTTGGTAAAGAGTTCCAGACGTTCAAAGTCTCCTTTATGTTTAAAGATGCCTCTATAAAAGGAGG
TAAGCAGCATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

99.476

100

0.995
  pilN Vibrio cholerae strain A1552

60.452

92.67

0.56

Multiple sequence alignment