Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   ACINK7_RS18125 Genome accession   NZ_CP173170
Coordinates   4197344..4197793 (-) Length   149 a.a.
NCBI ID   WP_404357457.1    Uniprot ID   -
Organism   Methylotuvimicrobium sp. KM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4192344..4202793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACINK7_RS18105 (ACINK7_18105) - 4193197..4193682 (-) 486 WP_404357449.1 hypothetical protein -
  ACINK7_RS18110 (ACINK7_18110) - 4193679..4193981 (-) 303 WP_404357451.1 nucleotidyltransferase domain-containing protein -
  ACINK7_RS18115 (ACINK7_18115) - 4194355..4194909 (-) 555 WP_404357453.1 Uma2 family endonuclease -
  ACINK7_RS18120 (ACINK7_18120) - 4195187..4197118 (-) 1932 WP_404357455.1 hypothetical protein -
  ACINK7_RS18125 (ACINK7_18125) pilA2 4197344..4197793 (-) 450 WP_404357457.1 pilin Machinery gene
  ACINK7_RS18130 (ACINK7_18130) rpmE 4198885..4199085 (-) 201 WP_084685514.1 50S ribosomal protein L31 -
  ACINK7_RS18135 (ACINK7_18135) - 4199699..4201033 (-) 1335 WP_404357459.1 AmpG family muropeptide MFS transporter -
  ACINK7_RS18140 (ACINK7_18140) nhaD 4201089..4202426 (-) 1338 WP_404361474.1 sodium:proton antiporter NhaD -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15520.77 Da        Isoelectric Point: 7.7766

>NTDB_id=1070397 ACINK7_RS18125 WP_404357457.1 4197344..4197793(-) (pilA2) [Methylotuvimicrobium sp. KM1]
MQKFKTQQGFTLIELMIVVAIIGILAAVALPAYQDYTIRAQVSEGPVLAGGVKTTVSEFWSDRGTLTGADSGAFGIPAAA
DITGNYVSQVAVANGLVTITYSSTDPRKASSQIDGSTLLLSPTTTVGSLIWTCKTGSNLPAKYLPSSCR

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1070397 ACINK7_RS18125 WP_404357457.1 4197344..4197793(-) (pilA2) [Methylotuvimicrobium sp. KM1]
ATGCAAAAATTTAAAACACAACAAGGTTTTACCTTGATCGAACTGATGATCGTGGTCGCGATCATCGGTATTTTGGCGGC
GGTCGCGCTGCCGGCTTATCAGGATTACACAATTCGAGCCCAAGTTTCTGAAGGTCCTGTTTTAGCAGGGGGGGTGAAAA
CTACAGTTTCTGAGTTTTGGAGCGATCGTGGTACTTTAACTGGTGCTGATAGTGGTGCGTTTGGTATCCCTGCAGCCGCA
GACATTACGGGTAATTATGTCAGTCAAGTAGCGGTTGCGAACGGACTAGTGACTATCACGTATAGTAGTACTGATCCAAG
AAAAGCTAGCTCACAAATTGATGGTTCTACATTATTGCTATCGCCTACAACTACTGTAGGAAGTCTAATCTGGACATGTA
AGACTGGTTCTAATTTACCGGCAAAATATCTGCCTTCAAGTTGTCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(39-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(5-30)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

51.37

97.987

0.503
  pilE Neisseria gonorrhoeae strain FA1090

46.203

100

0.49
  pilA2 Legionella pneumophila strain ERS1305867

49.306

96.644

0.477
  pilE Neisseria gonorrhoeae MS11

44.937

100

0.477
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.043

100

0.47
  pilA/pilA1 Eikenella corrodens VA1

40.252

100

0.43
  comP Acinetobacter baylyi ADP1

40.26

100

0.416
  pilA Ralstonia pseudosolanacearum GMI1000

36.095

100

0.409
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.216

100

0.403
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.931

97.315

0.369

Multiple sequence alignment